Abstract
For defining subareas in the human substantia nigra (SN) and ventral tegmental area (VTA) five brain stems of patients with no history of neurological disorders, varying in age between 60 and 91 years (Table 1), were used. The brain material was obtained at routine autopsy and fixed by immersion in 4% buffered formaldehyde for several months. On gross examination the brains did not show major abnormalities. The brain stems (from the middle of the mamillary body through the pons) were embedded in paraffin and cut transversely, i.e., perpendicular to the longitudinal axis of the brain stem, into 20 μm sections with a Reichert-Jung 2050 microtome. The sections were stained according to Nissl (cresylecht violet) and Klüver and Barrera (1953). In addition one horizontally sectioned brain stem was available.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
van Domburg, P.H.M.F., ten Donkelaar, H.J. (1991). Materials and Techniques. In: The Human Substantia Nigra and Ventral Tegmental Area. Advances in Anatomy Embryology and Cell Biology, vol 121. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75846-1_2
Download citation
DOI: https://doi.org/10.1007/978-3-642-75846-1_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-52823-4
Online ISBN: 978-3-642-75846-1
eBook Packages: Springer Book Archive