Infection by Magnaporthe: An In Vitro Analysis

  • Richard J. Howard
  • Timothy M. Bourett
  • Margaret A. Ferrari


Rice blast disease, described over 300 years ago, has been the subject of research in plant pathology labs throughout the world [17]. The fungus Magnaporthe grisea (anamorph, Pyricularia sp.), an ascomycete, has been of considerable interest because of its global agronomic impact in inciting blast disease and because it is recognized as one of the most widely disseminated foliar pathogens. In recent years M. grisea has become more valuable for investigation because it can be genetically manipulated and characterized [5, 7, 9, 15, 18, 23–25]. For these reasons we have undertaken the task of redefining the early events of host-pathogen interaction: inoculation, pre-penetration and penetration, all prerequisites of infection. This chapter briefly summarizes several new and significant findings of an in vitro analysis concerning conidial adhesion, appressorium development, and the mechanism of penetration. Many of these new findings can be ascribed to our use of freeze substitution [12] for sample preparation.


Germ Tube Rice Blast Appressorium Formation Rice Blast Fungus Host Surface 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • Richard J. Howard
    • 1
  • Timothy M. Bourett
    • 1
  • Margaret A. Ferrari
    • 1
  1. 1.DuPont Experimental StationWilmingtonUSA

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