Abstract
Spinal cord function involves complex interactions between spinal neurons, segmental input from primary afferent fibers, and suprasegmental input from descending pathways. Various neurotransmitters are thought to be involved in spinal cord function, and many studies have shown that the distribution of neurotransmitters in the spinal cord differs from that in other parts of the central nervous system. Perhaps there is also a difference in the types and densities of the neurotransmitter receptors; but in this respect, only limited information is available, and it derives mainly from receptor binding studies with radiolabeled ligands. Here we describe an alternative approach, using oocytes of the South African clawed frog, Xenopus laevis [9, 10].
This work was supported by grant R01-NS23284 from the U.S. public Health Service
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Akagi H, Miledi R (1988) Expression of glycine and other amino acid receptors by rat spinal cord mRNA in Xenopus oocytes. Neurosci Lett 95: 262–268
Akagi H, Miledi R (1988) Heterogeneity of glycine receptors and their messenger RNAs in rat brain and spinal cord. Science 242: 270–273
Gordon D, Merrick D, Auld V, Dunn R, Goldin AL, Davidson N, Catterall WA (1987) Tissue-specific expression of the RI and RI/ sodium channel subtypes. Proc Natl Acad Sci USA 84: 8682–8686
Gundersen CB, Miledi R, Parker I (1983) Voltage-operated channels induced by foreign messenger RNA in Xenopus oocytes. Proc R Soc Lond B 220: 131–140
Gundersen CB, Miledi R, Parker I (1984) Glutamate and kainate receptors induced by rat brain messenger RNA in Xenopus oocytes. Proc R Soc Lond B 221: 127–143
Kusano K, Miledi R, Stinnakre J (1982) Cholinergic and cathecolaminergic receptors in the Xenopus oocyte membrane. J Physiol (Lond) 328: 143–170
Miledi R (1982) A calcium-dependent transient outward current in Xenopus laevis oocytes. Proc R Soc Lond B 215: 491–497
Miledi R, Sumikawa K (1982) Synthesis of cat muscle acetylcholine receptors by Xenopus oocytes. Biomed Res 3: 390–399
Miledi R, Parker I, Sumikawa K (1989) Transplanting receptors from brains into oocytes. In: Smith J (ed) Fidia award lecture series. Raven, New York pp. 57–90
Sumikawa K, Parker I, Miledi R (1984) Partial purification and functional expression of brain mRNAs coding for neurotransmitter receptors and voltage-operated channels. Proc Natl Acad Sci USA 81: 7994–7998
Akagi H, Miledi R (1989) Discrimination of heterogenous mRNAs encoding strychnine-sensitive glycine receptors in Xenopus oocytes by antisense oligonucleotides. Proc Natl Acad Sci USA 86: 8103–8107
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Akagi, H., Miledi, R. (1991). Neurotransmitter Receptors and Voltage-Operated Channels Expressed by Rat Spinal Cord mRNA in Xenopus Oocytes. In: Shimoji, K., Kurokawa, T., Tamaki, T., Willis, W.D. (eds) Spinal Cord Monitoring and Electrodiagnosis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75744-0_8
Download citation
DOI: https://doi.org/10.1007/978-3-642-75744-0_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-75746-4
Online ISBN: 978-3-642-75744-0
eBook Packages: Springer Book Archive