Spinal cord function involves complex interactions between spinal neurons, segmental input from primary afferent fibers, and suprasegmental input from descending pathways. Various neurotransmitters are thought to be involved in spinal cord function, and many studies have shown that the distribution of neurotransmitters in the spinal cord differs from that in other parts of the central nervous system. Perhaps there is also a difference in the types and densities of the neurotransmitter receptors; but in this respect, only limited information is available, and it derives mainly from receptor binding studies with radiolabeled ligands. Here we describe an alternative approach, using oocytes of the South African clawed frog, Xenopus laevis [9, 10].
Spinal Cord Xenopus Oocyte Glycine Receptor Transient Outward Current Spinal Cord Function
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Akagi H, Miledi R (1988) Expression of glycine and other amino acid receptors by rat spinal cord mRNA in Xenopus oocytes. Neurosci Lett 95: 262–268PubMedCrossRefGoogle Scholar
Akagi H, Miledi R (1988) Heterogeneity of glycine receptors and their messenger RNAs in rat brain and spinal cord. Science 242: 270–273PubMedCrossRefGoogle Scholar
Gordon D, Merrick D, Auld V, Dunn R, Goldin AL, Davidson N, Catterall WA (1987) Tissue-specific expression of the RI and RI/ sodium channel subtypes. Proc Natl Acad Sci USA 84: 8682–8686PubMedCrossRefGoogle Scholar
Gundersen CB, Miledi R, Parker I (1983) Voltage-operated channels induced by foreign messenger RNA in Xenopus oocytes. Proc R Soc Lond B 220: 131–140PubMedCrossRefGoogle Scholar
Gundersen CB, Miledi R, Parker I (1984) Glutamate and kainate receptors induced by rat brain messenger RNA in Xenopus oocytes. Proc R Soc Lond B 221: 127–143PubMedCrossRefGoogle Scholar
Kusano K, Miledi R, Stinnakre J (1982) Cholinergic and cathecolaminergic receptors in the Xenopus oocyte membrane. J Physiol (Lond) 328: 143–170Google Scholar
Miledi R (1982) A calcium-dependent transient outward current in Xenopus laevis oocytes. Proc R Soc Lond B 215: 491–497PubMedCrossRefGoogle Scholar
Miledi R, Sumikawa K (1982) Synthesis of cat muscle acetylcholine receptors by Xenopus oocytes. Biomed Res 3: 390–399Google Scholar
Miledi R, Parker I, Sumikawa K (1989) Transplanting receptors from brains into oocytes. In: Smith J (ed) Fidia award lecture series. Raven, New York pp. 57–90Google Scholar
Sumikawa K, Parker I, Miledi R (1984) Partial purification and functional expression of brain mRNAs coding for neurotransmitter receptors and voltage-operated channels. Proc Natl Acad Sci USA 81: 7994–7998PubMedCrossRefGoogle Scholar
Akagi H, Miledi R (1989) Discrimination of heterogenous mRNAs encoding strychnine-sensitive glycine receptors in Xenopus oocytes by antisense oligonucleotides. Proc Natl Acad Sci USA 86: 8103–8107PubMedCrossRefGoogle Scholar