Neurotransmitter Receptors and Voltage-Operated Channels Expressed by Rat Spinal Cord mRNA in Xenopus Oocytes

  • H. Akagi
  • R. Miledi
Conference paper

Abstract

Spinal cord function involves complex interactions between spinal neurons, segmental input from primary afferent fibers, and suprasegmental input from descending pathways. Various neurotransmitters are thought to be involved in spinal cord function, and many studies have shown that the distribution of neurotransmitters in the spinal cord differs from that in other parts of the central nervous system. Perhaps there is also a difference in the types and densities of the neurotransmitter receptors; but in this respect, only limited information is available, and it derives mainly from receptor binding studies with radiolabeled ligands. Here we describe an alternative approach, using oocytes of the South African clawed frog, Xenopus laevis [9, 10].

Keywords

Sucrose Glycine Sedimentation Serotonin Fractionation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • H. Akagi
    • 1
    • 2
  • R. Miledi
    • 1
  1. 1.Laboratory of Cellular and Molecular Neurobiology, Department of PsychobiologyUniversity of CaliforniaIrvineUSA
  2. 2.Department of NeuroscienceMitsubishi Kasei Institute of Life SciencesMachida-shi, Tokyo 194Japan

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