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Identification of CD34-Positive Cells by Multiparameter Flow Cytometry

  • H. Sovalat
  • S. Serke

Abstract

In 1984, Civin et al. [1] generated a monoclonal antibody, MY-10, detecting an antibody expressed exclusively on the surface of hemopoietic cells. Subsequently, a number of further monoclonal antibodies with very similar specificities have been described by others: BI3C5 [2], ICH-3 [3], TUEK-3 [3], 12-8 [4], 8G12 [5], and QBEND-10 [3]. All these antibodies, partially detecting different epitopes, have been assigned to the cluster of differentiation 34 (CD34) [3]. Using FACS or immunomagnetic purging techniques, it has been shown that hemopoietic cells of all stages of differentiation express the CD34-antigen [6, 7, 8, 9]. Additionaly, Simmons & Torok-Storb [10] have shown that stroma cell percursors also express the CD34-antigen.

Keywords

Light Scatter Normal Bone Marrow Correlation Plot Multiparameter Flow Cytometry Differential Coexpression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwartz JF, Shaper JH (1984) Antigenic analysis of hematopoiesis: III. A hematopoietic progenitor cell surface antigen defined by monoclonal antibody raised against KG-la cells. Jornal of Immunology 133: 157–165Google Scholar
  2. 2.
    Katz FE, Tindle RT, Sutherland R, Greaves MF (1985) Identification of a membrane glycoprotein associated with haemopoietic progenitor cells. Leukemia Research 9: 191–198PubMedCrossRefGoogle Scholar
  3. 3.
    Civin CI, Trischmann TM, Fackler MJ, Bernstein ID, Bühring H-J, Campos L, Greaves MF, Kamoun M, Katz DR, Lansdorp PM, Look AT, Seed B, Sutherland DR, Tindle RW, Uchanska-Ziegler B (1989) Report on the CD34 cluster workshop. In: Knapp W, Dörken B, Rieber EP, Stein H, Gilks WR, Schmidt RE, von dem Borne AEGK (eds) Leukozyte typing IV. Oxford University Press, Oxford, pp 818–825Google Scholar
  4. 4.
    Andrews RG, Singer JW, Bernstein ID (1986) Monoclonal antibody 12-8 recognizes a 115- kd molecule present on both unipotent and multipotent hematopoietic colony-forming cells and their precursors. Blood 67: 842–845PubMedGoogle Scholar
  5. 5.
    Lansdorp PM, Dougherty GJ, Humphries RK (1989) CD34 epitopes. In: Leukocyte Typing IV, see reference 3Google Scholar
  6. 6.
    Serke S, Abe Y, Kirsch A, Huhn D (1991) Phenotyping of peripheral blood hemopoietic progenitor cells - in vitro cultures using CD34-/CD33-immunomagnetic purging. European Journal of Haemotology 47: 361–366CrossRefGoogle Scholar
  7. 7.
    Bühring H-J, Asenbauer B, Katrilaka K, Hummel G, Busch FW (1989) Sequential expression of CD34 and CD33 antigens on myeloid colony-forming cells. European Jornal of Haematology 42: 143–149CrossRefGoogle Scholar
  8. 8.
    Terstappen LWMM, Huang S, Safford M, Lansdorp PM, Loken MR (1991) Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34 CD38 progenitor cells. Blood 77: 1218–1227PubMedGoogle Scholar
  9. 9.
    Bernstein ID, Leary AG, Andrews RG, Ogawa M (1991) Blast colony-forming cells and precursors of colony-forming cells detectable in long-term marrow culture express the same phenotype (CD33− CD34−). Experimental Hematology 19: 680–682PubMedGoogle Scholar
  10. 10.
    Simmons PJ, Torok-Storb B (1991) CD34 expression by stromal precursors in normal human adult bone marrow. Blood 78: 2848–2853PubMedGoogle Scholar
  11. 11.
    Serke S, Säuberlich S, Abe Y, Huhn D (1991) Analysis of CD34-positive hemopoietic progenitor cells from normal human adult peripheral blood: flow cytometrical studies and in-vitro colony ( CFU-GM, BFU-E) assays. Annals of Hematology 62: 45–53PubMedCrossRefGoogle Scholar
  12. 12.
    Takaue Y, Kawano Y, Reading CL, Watanabe T, Abe T, Ninomiya T, Shimizu E, Ogura T, Kuroda Y, Yokobayashi A, Nakahata T, Asano S, Ventura G (1990) Effect of recombinant human G-CSF, GM-CSF, 11-3, and II-1@ on the growth of purified human peripheral blood progenitors. Blood 76: 330–335PubMedGoogle Scholar
  13. 13.
    Bender JG, Unverzagt KL, Walker DE, Lee W, Van Epps DE, Smith DH, Stewart ICC, To LB (1991) Identification and comparison of CD34-positive cells and their subpopula- tions from normal peripheral blood and bone marrow using multicolor flow cytometry. Blood 77: 2591–2596PubMedGoogle Scholar
  14. 14.
    Serke S, Säuberlich S, Huhn D (1991) Multiparameter flow-cytometrical of circulating CD34+−cells: correlation to the quantitation of circulating haemopoietic progenitor cells by in vitro colony-assay. British Journal of Haematology 77: 453–459PubMedCrossRefGoogle Scholar
  15. 15.
    Ema H, Suda T, Miura Y, Nakauchi H (1990) Colony formation of clone-sorted human hematopoietic progenitors. Blood 75: 1941–1946PubMedGoogle Scholar
  16. 16.
    Fritsch G, Emminger W, Buchinger P, Printz D, Gadner H (1991) CD34-positive cell proportions in peripheral blood correlate with colony-forming capacity. Experimental Haematology 19: 1079–1085Google Scholar
  17. 17.
    Wunder E, Sovalat H, Fritsch G, Silvestri F, Henon P, Serke S (1992) Reported on the “European workshop on peripheral blood stem cell determination & standardization”. Hematotherapy 2 (in press)Google Scholar
  18. 18.
    Neubauer A, Van Lessen A, Huhn D (1989) Two methods for determining T cell subpopulations. Deutsche Medizinische Wochenschrift 114: 414–416PubMedCrossRefGoogle Scholar
  19. 19.
    De Paoli P, Reitano M, Battistin S, Castiglia C, Santini G (1984) Enumeration of human lymphocyte subsets by monoclonal antibodies and flow-cytometry: A comperative study using whole blood or mononuclear cells separated by density centrifugation. Journal of Immunological Methods 72: 349–358PubMedCrossRefGoogle Scholar
  20. 20.
    Siena S, Bregni M, Brando B, Belli N, Ravagnani F, Gandola L, Stern AC, Lansdorp PM, Bonadonna G, Gianni AM (1991) Flow cytometry for clinical estimation of circulating hematopoietic progenitors for autologous transplantation in cancer patients. Blood 77: 400–409PubMedGoogle Scholar
  21. 21.
    Brugger W, Bross K, Frisch J, Dem P, Weber B, Mertelsmann R, Kanz L (1992) Mobilization of peripheral blood progenitor cells by sequential administration of interleukin-3 and granulocyte-macrophage colony-stimulating factor following polyche-motherapy with etoposide, ifosfamide, and cisplatin. Blood 79: 1193–1200PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • H. Sovalat
    • 1
  • S. Serke
    • 2
  1. 1.Institut de Recherche en Hematologie-Transfusion, Hôpital du HasenrainMulhouse CédexFrance
  2. 2.Hämatologische ZentrallaborUniversitätklinikum Rudolf-Virchow-CharlottenburgBerlin 19Germany

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