Identification of CD34-Positive Cells by Multiparameter Flow Cytometry

  • H. Sovalat
  • S. Serke


In 1984, Civin et al. [1] generated a monoclonal antibody, MY-10, detecting an antibody expressed exclusively on the surface of hemopoietic cells. Subsequently, a number of further monoclonal antibodies with very similar specificities have been described by others: BI3C5 [2], ICH-3 [3], TUEK-3 [3], 12-8 [4], 8G12 [5], and QBEND-10 [3]. All these antibodies, partially detecting different epitopes, have been assigned to the cluster of differentiation 34 (CD34) [3]. Using FACS or immunomagnetic purging techniques, it has been shown that hemopoietic cells of all stages of differentiation express the CD34-antigen [6, 7, 8, 9]. Additionaly, Simmons & Torok-Storb [10] have shown that stroma cell percursors also express the CD34-antigen.


Light Scatter Normal Bone Marrow Correlation Plot Multiparameter Flow Cytometry Differential Coexpression 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • H. Sovalat
    • 1
  • S. Serke
    • 2
  1. 1.Institut de Recherche en Hematologie-Transfusion, Hôpital du HasenrainMulhouse CédexFrance
  2. 2.Hämatologische ZentrallaborUniversitätklinikum Rudolf-Virchow-CharlottenburgBerlin 19Germany

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