Macrophages/Reticulum Cells in Early and Late Phases of Lymphogenous Metastasis

  • E. Vollmer
  • F. Shimamoto
  • V. Krieg
  • E. Grundmann
Part of the Current Topics in Pathology book series (CT PATHOLOGY, volume 84/2)


In recent years, increasing interest has focused on the role of macrophages/reticulum cells in the local immune defense against malignant tumors. Within the peritumoral field, these cells are supposed to bear the main functional burden, together with lymphocytes (for reviews see Macher and Sorg 1986; Roitt et al. 1985). At present information is still scarce about their functional role in the lymph nodes during lymphogenous metastasis (Fossum and Ford 1985; Meyer 1982; Syrjänen 1982). Besides their well-known phagocytic properties, cytotoxic, cytostatic, and cytolytic capacities may be involved. In addition, there are interactions between macrophages/reticulum cells and both T and B lymphocytes during immune defense, and so macrophages/reticulum cells may be regarded as accessory cells in the induction of the tumor-associated immune response.


Gastric Carcinoma Mononuclear Phagocyte System Subcapsular Sinus Neck Dissection Specimen Interdigitating Reticulum Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Araki K (1982) The ultrastructural evaluation of experimentally induced cancer cell invasion into the lymphatic vessel in rats. Bull Osaka Med Sch 28: 7–13PubMedGoogle Scholar
  2. Ausprunk DH, Folkman J (1977) Migration and proliferation of endothelial cells in preformed and newly formed blood vessels during tumor angiogenesis. Microvasc Res 14: 53–65PubMedCrossRefGoogle Scholar
  3. Barclay AN (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology 42: 593–600PubMedGoogle Scholar
  4. Baroni CD, Vitolo D, Remotti D, Biondi A, Pezzella F, Ruco LP, Uccini S (1987) Immunohistochemical heterogeneity of macrophage subpopulations in human lymphoid tissues. Histopathology 11: 1029–1042PubMedCrossRefGoogle Scholar
  5. Carter RL (1982) Some aspects of the metastatic process. J Clin Pathol 35: 1041–1049PubMedCrossRefGoogle Scholar
  6. Cordell JL, Falini B, Erber WN et al. (1984) Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase (APAAP complexes). J Histochem Cytochem 32: 219–229PubMedCrossRefGoogle Scholar
  7. Druckrey H, Preussmann R, Matzkies F, Invankovic S (1967) Selektive Erzeugung von Darmkrebs bei Ratten durch 1,2-Dimethylhydrazin. Naturwissenschaften 54: 285–286PubMedCrossRefGoogle Scholar
  8. Falk S, Müller H, Hashimoto H, Stutte HJ (1986) Immunhistochemische Untersuchungen zur Verteilung and Proliferationsaktivität von Zellen des lymphoretikulären Systems der Milz. Verh Dtsch Ges Pathol 70: 436Google Scholar
  9. Fisher B, Fisher ER (1966) Transmigration of lymph nodes by tumor cells. Science 152:1397–1398PubMedCrossRefGoogle Scholar
  10. Fisher B, Fisher ER (1967) Barrier function of lymph node to tumor cells and erythrocytes. Cancer 20: 1907–1913PubMedCrossRefGoogle Scholar
  11. Fossum S, Ford WL (1985) The organization of cell populations within lymph nodes: their origin, life history and functional relationships. Histopathology 9: 469–499PubMedCrossRefGoogle Scholar
  12. Gratzner HG (1982) Monoclonal antibody to 5-bromo-and 5-iododeoxyuridine. A new reagent for detection of DNA replication. Science 218: 474–475PubMedCrossRefGoogle Scholar
  13. Grundmann E (1984) Die Imyphogene Metastasierung. Verh Dtsch Ges Pathol 68: 33–46Google Scholar
  14. Grundmann E, Vollmer E (1985) Early local reaction and lymph node permeation of rat carcinoma HH9-cl 14 cells. An immunohistological approach. Pathol Res Pract 179: 304–309PubMedCrossRefGoogle Scholar
  15. Grundmann E, Vollmer E (1988) Die ersten Schritte der ymphknotenmetastasierung maligner Tumoren. In: Hilgarth M, Mönig-Schuth M (eds) Festschrift für Prof. Dr. H.-G. Hillemanns zum 65. Geburtstag. Druckerei am Fischmarkt, Konstanz, pp 163–174Google Scholar
  16. Guettier C, Gatter KC, Heryet A, Mason DY (1986) Dendritic reticulum cells in reactive lymph nodes and tonsils: an immunohistological study. Histopathology 10: 15–24PubMedCrossRefGoogle Scholar
  17. Hagemeier HH, Vollmer E, Goerdt S, Schulze-Osthoff K, Sorg C (1986) A monoclonal antibody reacting with endothelial cells of budding vessels in tumors and inflammatory tissues, and non-reacting with normal tissues. Int J Cancer 38: 481–488PubMedCrossRefGoogle Scholar
  18. Hansmann ML, Radzun HJ, Kaiserling E, Parwaresch MR (1984) Immunoelectron microscopic demonstration of tissue antigens with monoclonal antibodies. Virchows Arch [B] 46: 1–12CrossRefGoogle Scholar
  19. Hewitt HB, Blake ER (1975) Quantitative studies of translymphnodal passage of tumor cells naturally disseminated from a non-immunogenic murine squamous carcinoma. Br J Cancer 31: 25–35PubMedCrossRefGoogle Scholar
  20. Kamperdijk EWA, Raaymakers EM, de Leeuw JHS, Hoefsmit ECM (1978) Lymph node macrophages and reticulum cells in the immune response. I. The primary response to paratyphoid vaccine. Cell Tissue Res 192: 1–23PubMedCrossRefGoogle Scholar
  21. Kreipe H, Radzun HJ, Parwaresch MR, Haislip A, Hansmann ML (1987) Ki-M7 monoclonal antibody specific for myelomonocytic cell lineage and macrophages in human. J Histochem Cytochem 35: 1117–1126PubMedCrossRefGoogle Scholar
  22. Laurén P (1965) The two histological main types of gastric carcinoma: diffuse and so-called intestinal type carcinoma. Acta Pathol Microbiol Scand 64: 31–49PubMedGoogle Scholar
  23. Macher E, Sorg C (eds) (1986) Local immunity in cancer. Regensberg and Biermann, MünsterGoogle Scholar
  24. Mason DW, Arthur RP, Dallmann MJ, Green JR, Spickett GP, Thomas ML (1983) Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies. Immunol Rev 74: 57–82PubMedCrossRefGoogle Scholar
  25. Meyer EM (1982) Zur funktionellen Bedeutung der histologischen Lymphknotenreaktionen. Klin Wochenschr 60: 265–273PubMedCrossRefGoogle Scholar
  26. Nieuwenhuis P, Opstelten D (1984) Functional anatomy of germinal centers. Am J Anat 170: 421–435PubMedCrossRefGoogle Scholar
  27. Parwaresch MR, Radzun HJ, Feller AC, Peters KP, Hansmann ML (1983) Peroxidase-positive mononuclear leukocytes as possible precursors of human dendritic reticulum cells. J Immunol 131: 2719–2725PubMedGoogle Scholar
  28. Parwaresch MR, Radzun HJ, Kreipe H, Hansmann ML, Barth J (1986) Monocyte/macrophage-reactive monoclonal antibody Ki-M 6 recognizes in intracytoplasmic antigen. Am J Pathol 125: 141–151PubMedGoogle Scholar
  29. Radzun HJ (1985) Immunhistochemie des menschlichen mononukleär-phagozytischen Systems. Veröff Pathologie 124, G. Fischer Verlag, StuttgartGoogle Scholar
  30. Radzun HJ (1988) Differenzierungslinien im Monozyten/Makrophagensystem. Verh Dtsch Ges Pathol 72: 50–56PubMedGoogle Scholar
  31. Radzun HJ, Parwaresch MR (1983) Differential immunohistochemical resolution of the human mononuclear phagocyte system. Cell Immunol 82: 174–183PubMedCrossRefGoogle Scholar
  32. Radzun HJ, Parwaresch MR, Feller AC, Hansmann ML (1984) Monocyte/macrophage-specific monoclonal antibody Ki-M 1 recognizes interdigitating reticulum cells. Am J Pathol 117: 441–450PubMedGoogle Scholar
  33. Radzun Hi, Parwaresch MR, Bödewadt S, Sundström C, Lennert K (1985) Bimodal differentiation prospectives for promyelocytes. J Natl Cancer Inst 75: 199–206PubMedGoogle Scholar
  34. Radzun HJ, Kreipe H, Bödewadt AM, Hansmann ML, Barth J, Parwaresch MR (1987) Ki-M 8 monoclonal antibody reactive with an intracytoplasmic antigen of monocyte/macrophage lineage. Blood 69: 1320–1327PubMedGoogle Scholar
  35. Radzun HJ, Kreipe H, Zavazava N, Hansmann ML, Parwaresch MR (1988) Diversity of the human monocyte/macrophage system as detected by monoclonal antibodies. J Leukocyte Biol 43: 41–50PubMedGoogle Scholar
  36. Roitt J, Brostoff J, Male D (1985) Immunology. Churchill Livingstone/Gower Medical LondonGoogle Scholar
  37. Shimamoto F, Vollmer E (1987) Changes in intestinal mucosa above lymph follicles during carcinogenesis in rats. J Cancer Res Clin Oncol 113: 41–50PubMedCrossRefGoogle Scholar
  38. Shimamoto F, Tähara E, Yanaihara N (1983) Gut endocrine cells in rat intestinal tract carcinoma induced by 1,2-dimethylhydrazine. J Cancer Res Clin Oncol 105: 221–230PubMedCrossRefGoogle Scholar
  39. Stein H, Gerdes J, Mason DY (1982) The normal and malignant germinal centre. Clin Haematol 11: 531–559PubMedGoogle Scholar
  40. Syrjänen KJ (1982) The lymph nodes. Reactions to experimental and human tumors. Exp Pathol Suppl 8Google Scholar
  41. Tokuyasu KT (1980) Immunohistochemistry on ultrathin frozen sections. Histochem J 12: 381–403PubMedCrossRefGoogle Scholar
  42. Tokuyasu KT (1986) Cryosections for immunohistochemistry: In: Imura T, Maruse S, Suzuki T (eds) Proceedings of the 11th International Congress on Electron Microscopy, Kyoto, vol 3:1977–1978Google Scholar
  43. van Furth R (1982) Current view on the mononuclear phagocyte system. Immunobiology 161: 178–185PubMedCrossRefGoogle Scholar
  44. van Furth R, Cohn ZA, Hirsch JG, Humphrey JH, Spector WG, Langevoort HL (1972) The mononuclear phagocyte system: a new classification of macrophages, monocytes, and their precursor cells. Bull WHO 46: 845–852PubMedGoogle Scholar
  45. Vollmer E, Meyer EM (1986) Funktion und Reaktionsmuster von Lymphknoten in der Tùmorabwehr. GBK-Mitteilungsdienst NF H. 49: 55–59Google Scholar
  46. Vollmer E, Hölzel F, Lingemann J, Happe J (1986a) Verteilungs-und Reaktionsmuster in den Lymphknoten bei der lymphogenen Metastasierung des Ratten-Ca. HH 9-cl 14. Verh Dtsch Ges Pathol 70: 599Google Scholar
  47. Vollmer E, Kallin B, Krieg V, Hölzel F, Grundmann E (1986b) Immunhistologische Untersuchungen zum T-Lymphozytengehalt im regressiven Mammakarzinom HH 9-cl 14 und den regionären Lymphknoten der Ratte. Verh Dtsch Ges Pathol 70: 422Google Scholar
  48. Vollmer E, Shimamoto F, Krieg V, Grundmann E (1986c) Macrophages and T lymphocytes infiltrating the rat mammary carcinoma HH9-cl 14 in progressive and regressive tumor growth. J Cancer Res Clin Oncol 111: 13–19PubMedCrossRefGoogle Scholar
  49. Vollmer E, Hagemeier HH, Sorg C (1987) Follikuläre dendritische Retikulumzellen ( DRZ) des Lymphknotens als präneoplastisches Signal bei der tumorinduzierten Angiogenese. Verh Dtsch Ges Pathol 71: 385Google Scholar
  50. Vollmer E, Roessner A, Wuismann P, Härle A, Grundmann E (1989) The proliferation behavior of bone tumors investigated with the monoclonal antibody Ki-67. Curr Top Pathol 80: 91–114PubMedGoogle Scholar
  51. Wacker HH (1984) Umsatzkinetik der Gewebsmakrophagen. Anwendung makrophagenspezifischer monoklonaler Antikörper und Autoradiographie. Verh Dtsch Ges Pathol 68: 467Google Scholar
  52. Wacker HH, Radzun HJ, Parwaresch MR (1986) Initialmechanismen der humoralen Immunantwort. Verh Dtsch Ges Pathol 70: 501Google Scholar
  53. Wacker HH, Radzun HJ, Mielke V, Parwaresch MR (1987) Selective recognition of rat follicular dendritic cells (dendritic reticulum cells) by a new monoclonal antibody Ki-M4R in vitro and in vivo. J Leukocyte Biol 41: 70–77PubMedGoogle Scholar
  54. Wallace AC, Josephson RL, Hollenberg NK (1980) Observations on lymph node metastases. In: Grundmann E (ed) Metastatic tumor growth. Cancer Campaign, vol 4. Gustav Fischer, Stuttgart, pp 83–99Google Scholar
  55. Weibel ER, Elias H (1967) Quantitative Methoden in der Morphologie. Springer, Berlin Heidelberg New YorkGoogle Scholar
  56. Zwadlo G, Bröcker EB, v. Bassewitz DB, Feige U, Sorg C (1985) A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes. J Immunol 134: 1487–1491PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • E. Vollmer
  • F. Shimamoto
  • V. Krieg
  • E. Grundmann

There are no affiliations available

Personalised recommendations