Abstract
There is no doubt about the value of PGM 1 typing of bloodstains. The first investigations using starch gel electrophoresis were done by Culliford (1967), Heidel (1958) and Brinkmann (1969). The amount of stain needed was extremely large (between 10 and 50 mg of dried blood). With the introduction of agarose gel electrophoresis (Monn 1968; Culliford 1971; Grunbaum 1974) and cellulose acetate electrophoresis (Zajac and Sprague 1975) the quantity of stain could be reduced considerably. Isoelectric focusing allowed subtyping of PGM 1 of fresh blood and stains with a significantly higher value of information than before (Bark et al. 1976). Especially Berg et al. (1979, 1981), Schwerd and Hein (1983), Rittner et al. (1981), Oya et al. (1934) and Lawton and Kerr (1934) pointed out that additional or conversional bands occur with old blood samples respectively artificially prepared bloodstains as well as with cadaver tissue, thus leading to a false zymograms interpretation in some cases. Such conversional bands were not reported on in the few field studies published so far (blood- and seminal stains: Budowle et al. 1986; seminal stains: Oya et al. 1982). Therefore further examinations with practical cases seemed to be desirable.
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Bark JE, Harris MJ, Firth M (1976) Typing of the Common Phosphoglucomutase Variants Using Isoelectric Focusing – A New Interpretation of the Phosphoglucomutase System. J Forensic sci Soc 16: 115–120
Berg S, Ladiges ML, Ladiges O (1981) Der Einfluß von Blutproben- und Spurenalterung auf das PGM 1– und Gc-Subtypenmuster. Z Rechtsmed 87: 85–94
Berg S, Ladiges O, Ladiges ML (1979) Über die Nachweisbarkeit der Tf c– und PGM 1-Subtypen in gealterten Blutproben. Arch Kriminol 164: 101–106
Brinkmann B (1969) Bestimmung der Phosphoglucomutasetypen aus Blutproben. Dtsch Z Ges Gerichtl Med 66: 31–34
Budowle B, Murch RS, Davidson LC, Gambel AM, Kearny JJ (1986) Subtyping Phosphoglucomutase 1 in Semen Stains and Bloodstains: A Report on the Method. J Forensic sci Soc 31: 1341–1348
Culliford BJ (1971) The Examination and Typing of Bloodstains in the Crime Laboratory. Law Enforcement Assistance Administration, Washington, D.C.
Culliford BJ (1967) The Determination of Phosphoglucomutase (PGM) Types in Bloodstains. J Forensic sci Soc 7: 131–133
Grunbaum BW (1974) A Micro Procedure for Fast Typing of the Genetic Variants of Phosphoglucomutase. J Forensic sci Soc 14: 151–157
Heidel G (1968) Die spurenkundliche Bedeutung der Typen der sauren Erythrocyten- phosphatase. Dtsch Z Ges Gerichtl Med 63: 37–43
Lawton ME, Kerr LR (1984) Phosphoglucomutase Types in Blood and Hair Roots Taken from Post-Transfusion Subjects. J Forens sci 29: 445–448
Monn E (1968) A New Red Cell Phosphoglucomutase Phenotype in Man. Acta Genet 18: 123–127
Oya M, Kido A, Komatsu N, Shibata R (1984) Die Anwendung der Histoelektrofokussierung zur Bestimmung der PGM 1-Subtypen an menschlichen Körpergeweben. Z Rechtsmed 92: 225–230
Oya M, Tröger HD, Tutsch-Bauer E (1982) PGM 1-Fokussierung von Sperma aus Scheidenabstrichen. Beitr Gerichtl Med 40: 233–236
Rittner C, Fohlmeister M, Fittkau-Tombergs M (1981) Die PGM 1-Subtypen in der forensischen Spurenkunde. Krim Forens Wiss 44: 27–30
Schwerd W, Hein M (1983) Lagerungsbedingte Änderungen bei der Isofokussierung von Merkmalen der Phosphoglucomutase (PGM 1) in Blutproben und -spuren. Fortschritte der Rechtsmedizin, Springer, Berlin-Heidelberg-New York
Spielmann W, Kühnl P (1982) Blutgruppenkunde. Thieme, Stuttgart-New York
Zajac PL, Sprague AE (1975) Typing of Phosphoglucomutase (PGM) Variants in Dried Bloodstains by the Grunbaum Method of Cellulose Acetate Electrophoresis. J Forensic sci Soc 15: 69–74
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© 1990 Springer-Verlag Berlin Heidelberg
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Koch, KU., Hußlein, EM., Lötterle, J. (1990). Practical Value of PGM 1 Subtyping in Blood- and Semen Stains and Hair Roots. In: Polesky, H.F., Mayr, W.R. (eds) 13th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft für forensische Hämogenetik e.V.) New Orleans, October 19–21, 1989. Advances in Forensic Haemogenetics, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75496-8_66
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DOI: https://doi.org/10.1007/978-3-642-75496-8_66
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