Abstract
Normalization is a necessary step in order to compare two spectra- Mostly point normalization at the maximum of absorbance is used. Other methods are area normalization or the use of regression techniques. Reflectance spectra in TLC are not obeying Bouguer-Lambert-Beer’s law, therefore normalization has to be done by two-dimensional linear regression or nonlinear regression using a saturation function. Both methods are leading to good results. Nonlinear regression needs more computing time and more sophisticated computer programming.
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© 1990 Springer-Verlag Berlin Heidelberg
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Ebel, S., Kang, J.S., Windmann, W. (1990). Normalization of in Situ-Spectra in Thin Layer Chromatography. In: Gasteiger, J. (eds) Software Development in Chemistry 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75430-2_23
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DOI: https://doi.org/10.1007/978-3-642-75430-2_23
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-52173-0
Online ISBN: 978-3-642-75430-2
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