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Cell Culture Techniques in Heart and Vessel Research pp 36–60Cite as

Adult Ventricular Rat Heart Muscle Cells

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Abstract

Isolated and cultured cardiomyocytes have facilitated a large variety of experimental approaches which either require cardiomyocyte material in great purity or direct experimental access to single cardiomyocytes. Techniques for the isolation of cardiomyocytes as well as those for primary cultures of such cells have been difficult to establish, due to a number of reasons including (a) In heart tissue the muscle cells are firmly connected to each other by intercalated discs [20] and the extracellular matrix network [57]; these connections are difficult to cleave without injuring the cells. (b) The cardiomyocyte is a large, polygonal, rigid cell that is more easily damaged by mechanical impact than are many other cell types. (c) The adult cardiomyocyte does not divide [40], thus the number of cells initially isolated cannot be increased in cultures [47].

This work was supported by the Deutsche Forschungsgemeinschaft (Pi 162/2-2).

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Authors
  1. H. M. Piper
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  2. A. Volz
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  3. P. Schwartz
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Editors and Affiliations

  1. Physiologisches Institut, Abteilung für Herz- und Kreislaufphysiologie der Universität Düsseldorf, Moorenstr. 5, D-4000, Düsseldorf 1, Germany

    H. M. Piper

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© 1990 Springer-Verlag Berlin Heidelberg

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Piper, H.M., Volz, A., Schwartz, P. (1990). Adult Ventricular Rat Heart Muscle Cells. In: Piper, H.M. (eds) Cell Culture Techniques in Heart and Vessel Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75262-9_3

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  • DOI: https://doi.org/10.1007/978-3-642-75262-9_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-75264-3

  • Online ISBN: 978-3-642-75262-9

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