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The Early Effects of Radiation on DNA pp 317–332Cite as

The Development of Chemical Damage of DNA in Aqueous Solution

  • Conference paper

Part of the book series: NATO ASI Series ((ASIH,volume 54))

Abstract

A summary of results obtained in our laboratory is given concerning the formation of free radicals produced in DNA and components of DNA following ionization or reaction with OH radicals in aqueous solution and the rate of their conversion and decay. In the case of laser excitation biphotonic ionization of the nucleobases takes place and the radicalcations primarily formed are converted to neutral base radicals by deprotonation or by reaction with water. The neutral base radicals have their highest free spin densities at the nitrogens and oxygens of the nucleobases (N- and O-radicals). In contrast reaction with OH radicals leads primarily to carbon radicals by addition to the double bonds of the nucleobases and to sugar radicals by H abstraction. The reactions, the rate of the conversion and the decay of these radicals in DNA in aqueous solution is discussed with special regard to the radicals leading to strand break (sb) formation. In cells the decay is dominated by the reaction of the DNA radicals with endogenous thiols.

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Author information

Authors and Affiliations

  1. Max-Planck-Institut für Strahlenchemie, Stiftstrasse 34–36, 4330, Mülheim a.d. Ruhr, Germany

    D. Schulte-Frohlinde & E. Bothe

Authors
  1. D. Schulte-Frohlinde
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  2. E. Bothe
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Editors and Affiliations

  1. Medical Research Council, Radiobiology Unit, Chilton, Didcot, Oxon, OX11 ORD, UK

    E. M. Fielden  & P. O’Neill  & 

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© 1991 Springer-Verlag Berlin Heidelberg

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Schulte-Frohlinde, D., Bothe, E. (1991). The Development of Chemical Damage of DNA in Aqueous Solution. In: Fielden, E.M., O’Neill, P. (eds) The Early Effects of Radiation on DNA. NATO ASI Series, vol 54. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75148-6_34

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  • DOI: https://doi.org/10.1007/978-3-642-75148-6_34

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-75150-9

  • Online ISBN: 978-3-642-75148-6

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