DNA Double-Strand Break Induction in Yeast by Heavy ION Irradiation

  • T. C. Akpa
  • K. J. Weber
  • J. Kiefer
  • M. Frankenberg-Schwager
  • R. Harbich
  • D. Frankenberg
Part of the NATO ASI Series book series (volume 54)

Abstract

DNA double-strand breaks are regarded as fundamental lesions in the genome of irradiated cells. In yeast lethal damage from sparsely ionizing radiation could conclusively be identified with this lesion type whereas for other systems the situation is less clear. Although there are data for some high LET radiations, mainly α-particles, it is still an open question whether DSB are also the determinants of cellular effects from heavy ion exposure. The first preliminary results on DSB induction in wild-type yeast (strain 211*B) exposed to accelerated charged particles are presented here. The irradiations were performed at the Gesellschaft für Schwerionenforschung (GSI, Darmstadt) using the UNILAC-accelerator. Exposure conditions and dosimetry were as described by Schöpfer et al. (1980). DSB were assessed by neutral sucrose sedimentation according to Frankenberg-Schwager et al. (1979). Measurements are so far available for neon, argon and lead ions of various energies up to about 20 MeV/u (see table 1).

Keywords

Sucrose Argon Sedimentation Neon 

References

  1. Christensen RC, Tobias CA, Taylor WD (1972) Heavy-ion induced single- and double-strand breaks in X-174 replicative form of DNA. Int J Radiat Biol 22:457–477CrossRefGoogle Scholar
  2. Ritter MA, Cleaver JE, and Tobias CA (1977) High-LET radiation induce a large proportion on non-rejoining DNA breaks. Nature 266:653–655PubMedCrossRefGoogle Scholar
  3. Neary GJ, Horgan VJ, Bance DA, Stretch A (1972) Further data on DNA strand breakage by various radiation qualities. Int J Radiat Biol 22:525–537CrossRefGoogle Scholar
  4. Neary GJ, Simpson-Gildemeister VFW, Peacocke AR (1970) Theinfluence of radiation quality and oxygen on strand breakage in dry DNA. Int J Radiat Biol 18:25–40CrossRefGoogle Scholar
  5. Kampf G, Eichhorn K (1983) DNA strand breakage by different radiation qualities and relations to cell killing: Further results after the influence of alpha-particles and carbon ions. Stud Biophys 93:17–26Google Scholar
  6. Frankenberg-Schwager M, Frankenberg D, Blöcher D, Adamczyk C (1979) The influence of oxygen on the survival and yield of DNA double-strand breaks in irradiated yeast cells. Int J Radiat Biol 36:261–270CrossRefGoogle Scholar
  7. Frankenberg D, Frankenberg-Schwager M, Blocher D, Harbich R (1981) Evidence for double-strand breaks as the critical lesions in yeast cells irradiated with sparsely or densely ionizing radiation under oxic and anoxic conditions. Radiat Res 88:524–532PubMedCrossRefGoogle Scholar
  8. Schöpfer F, Kiefer J, Schneider E, Kraft G (1980) Inactivation of dried yeast cells by accelerated heavy ions of very high LET. Radiat Res 82:235–243PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • T. C. Akpa
    • 1
  • K. J. Weber
    • 1
  • J. Kiefer
    • 1
  • M. Frankenberg-Schwager
    • 2
  • R. Harbich
    • 2
  • D. Frankenberg
    • 2
  1. 1.Strahlenzentrum der Justus-Liebig-UniversitätGiessenGermany
  2. 2.Abteilung für Biophysikalische Strahlenforschung der GSFFrankfurtGermany

Personalised recommendations