DNA Double-Strand Break Induction in Yeast by Heavy ION Irradiation
Part of the
NATO ASI Series
book series (volume 54)
DNA double-strand breaks are regarded as fundamental lesions in the genome of irradiated cells. In yeast lethal damage from sparsely ionizing radiation could conclusively be identified with this lesion type whereas for other systems the situation is less clear. Although there are data for some high LET radiations, mainly α-particles, it is still an open question whether DSB are also the determinants of cellular effects from heavy ion exposure. The first preliminary results on DSB induction in wild-type yeast (strain 211*B) exposed to accelerated charged particles are presented here. The irradiations were performed at the Gesellschaft für Schwerionenforschung (GSI, Darmstadt) using the UNILAC-accelerator. Exposure conditions and dosimetry were as described by Schöpfer et al. (1980). DSB were assessed by neutral sucrose sedimentation according to Frankenberg-Schwager et al. (1979). Measurements are so far available for neon, argon and lead ions of various energies up to about 20 MeV/u (see table 1).
KeywordsRadiation Quality Accelerate Charged Particle Irradiate Yeast Fundamental Lesion Irradiate Yeast Cell
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© Springer-Verlag Berlin Heidelberg 1991