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Post-Transcriptional Control of Gene Expression pp 487–495Cite as

Initiation Factors Involved in mRNA Binding to Ribosomes in Saccharomyces Cerevisiae

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Part of the book series: NATO ASI Series ((ASIH,volume 49))

Summary

The yeast Saccharomyces cerevisiae has become a model system for studies of eukaryotic translation. Amino acid similarity between yeast and mammalian translation factors and in vitro or in vivo functional assays suggest that the overall translation mechanism as well as individual factor polypeptides are evolutionary conserved and that findings in the yeast system are likely to be significant for our understanding of translation in mammalian cells. To investigate the function of translation initiation factors and their subunits we have prepared in vitro systems which are dependent on a single factor. We have obtained factor-dependent lysates by two approaches: (i) Controlled in vivo expression of a factor proposed to be involved in unwinding mRNA secondary structure, initiation factor 4A (eIF-4A). An eIF-4A-dependent in vitro translation system was then prepared from cells in which transcription of the eIF-4A gene was repressed, (ii) A yeast strain was constructed containing a mutant cap-binding factor (eIF-4E). In lysates prepared from such cells translation of capped mRNA was dependent on the addition of purified eIF-4E.

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Author information

Authors and Affiliations

  1. Institut für Biochemie und Molekularbiologie, Universität Bern, Bühlstrasse 28, CH-3012, Bern, Switzerland

    P. P. Müller, S. Blum, M. Altmann, S. Lanker & H. Trachsel

Authors
  1. P. P. Müller
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  2. S. Blum
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  3. M. Altmann
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  4. S. Lanker
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  5. H. Trachsel
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Editor information

Editors and Affiliations

  1. Gesellschaft für Biotechnologische Forschung mbH, W-3300, Braunschweig, Germany

    John E. G. McCarthy

  2. Biological Laboratory, University of Kent, CT2 7NJ, Canterbury, Kent, England

    Mick F. Tuite

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© 1990 Springer-Verlag Berlin Heidelberg

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Müller, P.P., Blum, S., Altmann, M., Lanker, S., Trachsel, H. (1990). Initiation Factors Involved in mRNA Binding to Ribosomes in Saccharomyces Cerevisiae . In: McCarthy, J.E.G., Tuite, M.F. (eds) Post-Transcriptional Control of Gene Expression. NATO ASI Series, vol 49. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75139-4_45

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  • DOI: https://doi.org/10.1007/978-3-642-75139-4_45

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-75141-7

  • Online ISBN: 978-3-642-75139-4

  • eBook Packages: Springer Book Archive

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