Abstract
Antisense RNAs or oligodeoxynucleotides (oligos) have been used to suppress expression from specific genes during the development of various eukaryotic organisms, from the slime mold up to the mouse. We have been using oligos to ablate selected mRNAs in Xenopus oocytes. Using the endogenous histone H4 mRNA as a target we find that the degree of ablation obtained depends on the choice of oligo, and that the relative effectiveness of the different oligos can be mimicked in an in vitro reaction. Substitution of phosphorothioate for phosphodiester linkages in one oligo greatly increases its efficacy at low concentrations. Finally we describe experiments where the injection into oocytes of an oligo complementary to a new cdc2-related kinase mRNA whose cDNA we have recently cloned, accelerates the rate of progesterone-induced maturation.
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Colman, A., Baker, C., Shuttleworth, J. (1990). The Antisense Approach and Early Xenopus Development. In: McCarthy, J.E.G., Tuite, M.F. (eds) Post-Transcriptional Control of Gene Expression. NATO ASI Series, vol 49. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75139-4_11
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DOI: https://doi.org/10.1007/978-3-642-75139-4_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-75141-7
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