Abstract
The epidermal growth factor receptor (EGF-R) is a transmembrane glycoprotein of 170 kDa with an extracellular ligand binding domain, a single hydrophobic transmembrane stretch, and an intracellular domain containing protein tyrosine kinase activity (Ullrich et al., 1984). Binding of EGF to its receptor enhances this tyrosine kinase activity, thus inducing tyrosine phosphorylation of several protein substrates including the EGF-R itself (Hunter and Cooper, 1985). Activation of the EGF-R tyrosine kinase results in a cascade of biochemical and physiological responses, finally leading to stimulation of DNA synthesis and cell division in most cells (Carpenter, 1987). Using EGF-R mutants, the protein tyrosine kinase activity was shown to be essential for mitogenic signaling (Schlessinger, 1988a).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Basu, A., Raghunath, M., Bishayee, S., and Das, M. (1989) Mol Cell. Biol. 9, 671–677.
Basu, M., Sen-Majumdar, A., Basu, A., Murthy, U., and Das, M. (1986) J. Biol. Chem. 261, 12879–12882.
Bertics, P.J., and Gill, G.N. (1985) J. Biol. Chem. 260, 14642–14647.
Bishayee, S., Majumdar, S., Khire, J., and Das, M. (1989) J. Biol. Chem. 264, 11699–11705.
Biswas, R., Basu, M., Sen-Majumdar, A., and Das, M. (1985) Biochemistry 24, 3795–3802.
Boni-Schnetzler, M., and Pilch, P.F. (1987) Proc. Natl. Acad. Sci. USA 84, 7832–7836.
Carpenter, G. (1987) Annu. Rev. Biochem. 56, 881–914.
Cochet, C., Kashles, O., Chambaz, E.M., Borello, I., King, C.R., and Schlessinger, J. (1989) J. Biol. Chem. 263, 3290–3295.
Defize, L.H.K., Moolenaar, W.H., van der Saag, P.T., and de Laat, S.W. (1986) EMBO J. 5, 1187–1192.
Defize, L.H.K., Mummery, C.L., Moolenaar, W.H., and de Laat, S.W. (1987) Cell Diff. 20, 87–102.
Defize, L.H.K., Boonstra, J., Meisenhelder, J., Kruijer, W., Tertoolen, L.G.J., Tilly, B.C., Hunter, T., van Bergen en Henegouwen, P.M.P., Moolenaar, W.H., and de Laat, S.W. (1989) J. Cell Biol. 109, 2495–2507.
Fanger, B.O., Austin, K.S., Earp, H.S., and Cidlowski, J.A. (1986) Biochemistry 25, 6414–6420.
Fanger, B.O., Stephens, J.E., and Staros, J.V. (1989) FASEB J. 3, 71–75.
Gill, G.N., Kawamoto, T., Cochet, C., Le, A., Sato, J.D., Masui, H., McLeod, C., and Mendelsohn, J. (1984) J. Biol. Chem. 259, 7755–7760.
Gill, G.N., Bertics, P.J., and Santon, J.B. (1987) Mol. Cell. Endocrinol. 51, 169–186.
Greenfield, C., Hiles, I., Waterfield, M.D., Federwisch, M., Wollmer, A., Blundell, T.L., and McDonald, N. (1989) EMBO J. 8, 4115–4123.
Hammacher, A., Mellstrom, K., Heldin, C-H., and Westermark, B. (1989) EMBO J. 8, 2489–2495.
Heldin, C-H., Ernlund, A., Rorsman, C., and Ronnstrand, L. (1989) J. Biol. Chem. 264, 8905–8912.
Honegger, A.M., Kris, R.M., Ullrich, A., and Schlessinger, J. (1989) Proc. Natl. Acad. Sci. USA 86, 925–929.
Honegger, A.M., Schmidt, A., Ullrich, A., and Schlessinger, J. (1990) Mol. Cell. Biol. 10, 4035–4044.
Hunter, T., and Cooper, J.A. (1985) Annu. Rev. Biochem. 54, 897–930.
Koland, J.G., and Cerione, R.A. (1988) J. Biol. Chem. 263, 2230–2237.
Lyall, M., Zilberstein, A., Gazit, A., Gilon, C., Levitzki, A., and Schlessinger, J. (1989) J. Biol. Chem. 264, 14503–14509.
Northwood, I.C., and Davis, R.J. (1988) J. Biol. Chem. 263, 7450–7453.
O’Brien, R.M., Soos, M.A., and Siddle, K. (1987) EMBO J. 6, 4003–4010.
Schlessinger, J. (1986) J. Cell Biol. 103, 2067–2072.
Schlessinger, J. (1988a) Biochemistry 27, 3119–3123.
Schlessinger, J. (1988b) Trends Biochem. Sci. 13, 443–447.
Schreiber, A.B., Lax, I., Yarden, Y., Eshhar, Z., and Schlessinger, J. (1981) Proc. Natl. Acad. Sci. USA 78, 7535–7539.
Schreiber, A.B., Libermann, T.A., Lax, I., Yarden, Y., and Schlessinger, J. (1983) J. Biol. Chem. 258, 846–853.
Spaargaren, M., Defize, L.H.K., de Laat, S.W., and Boonstra, J. (1990) Biochem. Biophys. Res. Commun. 171, 882–889.
Spaargaren, M., Defize, L.H.K., Boonstra, J., and de Laat, S.W. (1991) J. Biol. Chem. 266, in press.
Ullrich, A., Coussens, L., Hayflick, J.S., Dull, T.J., Gray, A., Tam, A.W., Lee, J., Yarden, Y., Libermann, T.A., Schlessinger, J., Downward, J., Mayes, E.L.V., Whittle, N, Waterfield, M.D., and Seeburg, P.H. (1984) Nature 309, 418–425.
Ullrich, A., and Schlessinger, J. (1990) Cell 61, 203–212.
Weber, W., Bertics, P.J., and Gill, G.N. (1984) J. Biol. Chem. 259, 14631–14636.
Weiner, D.B., Liu, J., Cohen, J.A., Williams, W.V., and Greene, M.I. (1989) Nature 339, 230–231.
Yaish, P., Gazit, A., Gilon, C., and Levitzki, A. (1988) Science 242, 933–935.
Yarden, Y., and Schlessinger, J. (1987a) Biochemistry 26, 1434–1442.
Yarden, Y., and Schlessinger, J. (1987b) Biochemistry 26, 1443–1451.
Yarden, Y. (1990) Proc. Natl. Acad. Sci. USA 87, 2569–2573.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Spaargaren, M., Defize, L.H.K., Boonstra, J., de Laat, S.W. (1991). Dimerization Activates the Epidermal Growth Factor Receptor Tyrosine Kinase. In: Ross, E.M., Wirtz, K.W.A. (eds) Biological Signal Transduction. NATO ASI Series, vol 52. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75136-3_4
Download citation
DOI: https://doi.org/10.1007/978-3-642-75136-3_4
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-75138-7
Online ISBN: 978-3-642-75136-3
eBook Packages: Springer Book Archive