The Properties and Biological Action of Phytochrome: Prologue

  • B. Thomas
Conference paper
Part of the NATO ASI Series book series (volume 50)

Abstract

Plants possess sophisticated mechanisms for detecting light quality and quantity and adapting their patterns of growth and development depending upon their environment. This process of photomorphogenesis requires photoreceptor pigments which absorb light and provide the initial biochemical signal to trigger the plant’s photoresponse. The major photoreceptor in plants is phytochrome. This chromoprotein is found in all green plants including algae, mosses and ferns. It exists, characteristically, as two photoisomers called Pr and Pfr, each with a distinct absorbance spectrum. Typically, Pr has a major absorbance maximum (λmax) at about 660 nm and a secondary maximum at about 380 nm. Pfr on the other hand has a λmax at about 730 nm and a secondary peak at about 400 nm. When either form absorbs light a series of photochemical and protein conformational changes is initiated leading within milliseconds to the formation of the other photoisomer. For many responses red light at about 660 nm is the most effective part of the spectrum and the effect of red can be prevented by a subsequent far-red irradiation at about 730 nm. In such cases sequentially antagonistic actions of red and far-red can be seen over several cycles of irradiation. The effectiveness of Pfr-forming irradiation and reversal by Pr-forming irradiation leads inevitably to the conclusion that Pfr is the biologically active isomer and Pr an inactive form of phytochrome. It is worth bearing in mind that short alternating treatments with narrow waveband light, as used in these experiments is not necessarily comparable with natural irradiation. Nevertheless, the distinction between the biological activity of Pr and Pfr has provided the rationale underlying four decades of phytochrome research.

Keywords

Corn Chlorophyll Cysteine Germinate Half Life 

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References

  1. Abe H, Yamamoto KT, Nagatani A, Furuya M (1985) Characterization of green tissue-specific phytochrome isolated immunochemically from pea seedlings. Plant Cell Physiol. 26:1387–1399.Google Scholar
  2. Adamse P, Kendrick RE, Koorneef M (1988) Photomorphogenetic mutants of higher plants. Photochem. Photobiol. 48:833–841.CrossRefGoogle Scholar
  3. Boylan MT, Quail PH (1989) Oat phytochrome is biologically active in transgenic tomatoes. The Plant Cell 1:765–773.PubMedCrossRefGoogle Scholar
  4. Carr-Smith H, Thomas B, Johnson CB (1989) An action spectrum for the effects of continuous light on flowering in wheat. Planta 179:428–432.CrossRefGoogle Scholar
  5. Colbert JT (1988) Molecular biology of phytochrome. Plant Cell Environ. 11:305–318.CrossRefGoogle Scholar
  6. Colbert JT, Hershey HP, Quail PH (1985) Phytochrome regulation of phytochrome mRNA abundance. Plant Mol. Biol. 5:91–101.CrossRefGoogle Scholar
  7. Duke SO, Naylor AW, Wickliff JL (1977) Phytochrome control of longitudinal growth and phytochrome synthesis in maize seedlings. Physiol Plant 40:59–68.CrossRefGoogle Scholar
  8. Hershey HP, Colbert JT, Lissemore JL, Barker RF, Quail PH (1984) Molecular cloning of cDNA for Avena pbytochrome. Proc. Nat. Acad. Sci. U.S.A. 81:2332–2336.CrossRefGoogle Scholar
  9. Hilton JR, Thomas B (1985) A comparison of seed and seedling phytochrome in Avena sativa using monoclonal antibodies. J. Exp. Bot. 36:1937–1946.CrossRefGoogle Scholar
  10. Hilton JR, Thomas B (1987) Photoregulation of phytochrome synthesis in germinating embryos of Avena sativa L. J. Expt Bot 38:1704–1712.CrossRefGoogle Scholar
  11. Jenkins GI (1988) Photoregulation of gene expression in plants. Photochem. Photobiol. 48:821–832.CrossRefGoogle Scholar
  12. Kaufman L, Briggs WR, Thompson W (1985) Phytochrome control of specific mRNA levels in pea buds: the presence of both very low and low fluence responses. Plant Physiol. 78:388–393.PubMedCrossRefGoogle Scholar
  13. Kaufman L, Roberts LL, Briggs WR, Thompson W (1986) Phytochrome control of specific mRNA levels in developing pea buds: kinetics of accumulation, reciprocity and and escape kinetics of the low fluence response. Plant Physiol. 81:1033–1038.PubMedCrossRefGoogle Scholar
  14. Kay SA, Nagatani A, Keith B, Deak M, Furuya M, Chua N-H (1989) Rice phytochrome is biologically active in transgenic tobacco. The Plant Cell 1:775–782.PubMedCrossRefGoogle Scholar
  15. Keller JM, Shanklin J, Vierstra RD, Hershey HP (1989) expression of a factional monocotyledonous phytochrome in transgenic tobacco. EMBO J. 8:1005–1012.PubMedGoogle Scholar
  16. Konomi K, Abe H, Furuya M (1987) Changes in the content of phytochrome I and II apoproteins in embryonic axes of pea seeds during imbibition. Plant Cell Physiol. 28:1443–1451.Google Scholar
  17. Lagarias JC, Lagarias DM (1989) Self-assembly of synthetic phytochrome holoprotein in vitro. Proc. Nat. Acad. Sci. USA 86:5778–5780.PubMedCrossRefGoogle Scholar
  18. Lissemore JL, Quail PH (1988) Rapid transcriptional regulation by phytochrome of the genes for phytochrome and chlorophyll a/b-binding protein in Avena sativa. Mol. Cell. Biol. 8:4840–4850.PubMedGoogle Scholar
  19. Nagy F, Kay SA, Chua N-H (1988) Gene regulation by phytochrome. Trends Genet. 4:37–42PubMedCrossRefGoogle Scholar
  20. Rüdiger W (1986) The Chromophore. In: Kendrick RE, Kronenberg GHM (eds) Photomorphogenesis in Plants. Martinus Nihoff Publ, Dordrecht, The Netherlands. pl7.Google Scholar
  21. Sharrock RA, Parks BM, Koornneef M, Quail PH (1988) Molecular analysis of the phytochrome deficiency in an aurea mutant of tomato. Molec. Gen. Genet. 213:9–14.CrossRefGoogle Scholar
  22. Sharrock RA, Quail PH (1989) Novel phytochrome sequences in Arubidopsis thaliana: structure, evolution and differential expression of a plant regulatory photoreceptor family Gene. Develop. 3:1745–1757.Google Scholar
  23. Shimazaki Y, Pratt LH (1985) Immunochemical detection with rabbit polyclonal and mouse monoclonal antibodies of different pools of phytochrome from etiolated and green Avena shoots. Planta 164:333–344.CrossRefGoogle Scholar
  24. Thomas B (1991) Phytochrome properties and biological action. In: V Neuhoff (ed) Recent advances in cell to cell signalling. Springer-Verlag in press.Google Scholar
  25. Thomas B, Penn SE, Jordan BR (1989) Factors affecting phytochrome transcripts and apoprotein synthesis in germinating embryos of Avena sativa L. J. Expt. Bot. 40:1299–1304.CrossRefGoogle Scholar
  26. Tobin EM, Silverthorne J (1985) Light regulation of gene expression in higher plants. Annu. Rev. Plant Phys. 36:569–593.CrossRefGoogle Scholar
  27. Tokuhisa JG, Daniels SM, Quail PH (1985) Phytochrome in green tissue: spectral and immunochemical evidence for two distinct molecular species of phytochrome in light-grown Avena sativa. Planta 64:321–332.CrossRefGoogle Scholar
  28. Tokuhisa JG, Quail PH (1987) The levels of two distinct species of phytochrome are regulated differently during germination in Avena sativa L. Planta 172:371–377.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • B. Thomas
    • 1
  1. 1.Horticulture Research InternationalLittlehampton, West SussexUK

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