Natural Killer Cells and Their Precursors in Mice With Severe Combined Immunodeficiency
Natural Killer (NK) cells are capable of lysing tumor cells and virally infected cells and of regulating immune responses in the absence of apparent sensitization (Trinchieri, 1989). The precise relationship of NK cells to other lympho-hemopoietic cells is uncertain. Previous studies have failed to provide definitive evidence of NK cell lineage, since NK activity has been ascribed to a variety of cell types, including pre-T cells, basophils, and promonocytes. Indeed it could be argued that NK cells do not represent a homogeneous population with a distinct lineage. However, several recent studies have documented that NK killing by freshly isolated murine spleen cells is mediated by a morphologically homogeneous population (Hackett, et al. 196a) that has a characteristic phenotypic profile. Murine NK cells are NK-1.1+ /NK2.1+, Qa-5+, Thy-1±, AsGM-1+, CD4−, CD8−, CD3−, Jlld−, and IL-2R alpha (p55)−.
KeywordsSorting Stimul Hines Ionomycin Progen
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