Skip to main content
SpringerLink
Log in

Techniques and Applications of In Situ Hybridisation

  • Chapter
Pathology of the Nucleus

Part of the book series: Current Topics in Pathology ((CT PATHOLOGY,volume 82))

Abstract

During the last two decades, morphological investigations have changed considerably with the development of immunocytochemistry. However, the information gained by the demonstration of cellular antigens is limited as this technique does not give any insight into other intracellular metabolic processes. The introduction of in situ hybridisation (ISH) using nucleic acid probes provides further morphological understanding of gene expression in different physiological and pathological conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Allen JM, Sasek CA, Martin JB, Heinrich G (1987) Use of complementary 125I-labelled RNA for single cell resolution by in situ hybridisation. Biotechniques 5: 774–777

    CAS  Google Scholar 

  • Andrew SM, Jasani B (1987) An improved method for the inhibition of endogenous peroxidase nondeleterious to lymphocyte surface markers. Application to immunoperoxidase studies on eosinophil rich tissue perparations. Histochem J 19: 426–430

    PubMed  CAS  Google Scholar 

  • Angerer LM, Angerer RC (1981) Detection of poly A+ RNA in sea urchin eggs and embryos by quantitative in situ hybridisation. Nucleic Acids Res 9: 2819–2840

    PubMed  CAS  Google Scholar 

  • Angerer RC, Cox KH, Angerer LM (1985) In situ hybridisation to cellular RNAs. Genet Eng 7: 43–65

    CAS  Google Scholar 

  • Angerer LM, Stoler MH, Angerer RC (1987) In situ hybridisation with RNA probes: an annotated recipe. In: Valentino K, Erberwine JH, Barchas JD (eds) In situ hybridisation. Applications to neurobiology. Oxford University Press, Oxford pp 42–47

    Google Scholar 

  • Banttlow CE, Heumann R, Schwab ME, Thoenen H (1987) Cellular localisation of nerve growth factor synthesis by in situ hybridisation. EMBO J 6: 891–899

    Google Scholar 

  • Belasco JG, Nilsson G, von Gabain A, Cohen SN (1986) The stability of E. coli gene transcripts is dependent on determinants localised to specific mRNA segments. Cell 46: 245–251

    PubMed  CAS  Google Scholar 

  • Bhatt B, Burns J, Flamery D, McGee J (1988) Direct visualisation of single copy genes on banded metaphase chromosome by non-isotopic in situ hybridisation. Nucleic Acids Res 16: 3951–3961

    PubMed  CAS  Google Scholar 

  • Bloom FE, Naus CCG, Miller FD, Morrison JH (1988) Immunocytochemical and in situ hybridisation analysis of the development of the rat somatostatin-containing neocortical neuronal system. J Comp Neurol 269: 448–464

    PubMed  Google Scholar 

  • Bodkin DK, Knudson DL (1985) Assessment of sequence relatedness of double stranded RNA genes by RNA-RNA blot hybridisation. J Virol Methods 10: 45–52

    PubMed  CAS  Google Scholar 

  • Bollum FJ (1974) Terminal deoxynucleotidyl transferase. In: Boyer PD (ed) The enzymes. Academic Press, New York

    Google Scholar 

  • Brahic M, Haase AT (1978) Detection of viral sequences of low reiteration frequency by in situ hybridisation. Proc Natl Acad Sci USA 75: 6125–6129

    PubMed  CAS  Google Scholar 

  • Brahic M, Haase AT, Cash E (1984) Simultaneous in situ detection of viral RNA and antigens. Proc Natl Acad Sci USA 81: 5445–5448

    PubMed  CAS  Google Scholar 

  • Brawerman G (1987) Determinant of messenger RNA stability. Cell 48: 5–6

    PubMed  CAS  Google Scholar 

  • Brechot C, Degas F, Lugassy C (1985) Hepatitis B virus DNA in patients with chronic liver disease negative tests for hepatitis B surface antigen. N Engl J Med 312: 270–276

    PubMed  CAS  Google Scholar 

  • Bresser J, Evihger-Hodges MJ (1987) Comparison and optimisation of in situ hybridization procedures yielding rapid, sensitive mRNA detections. Gene AnalTechn 4: 89–104

    CAS  Google Scholar 

  • Brigati DJ, Myerson D, Leary J J et al. Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labelled hybridisation probes. Virology 126: 32–50

    Google Scholar 

  • Brahic M, Haase AT (1981) Lentivirinae: Maedi/uisna virus group infections. Comparative aspects and diagnosis. In: Kurstak E, Kurstak C (eds) Comparative diagnosis of viral diseases, vol IV, part B. Academic Press, New York, pp 619–643

    Google Scholar 

  • Britten RJ, Davidson EH (1985) Hybridisation strategy. In: Hames BD, Higgings SJ (eds) Nucleic acid hybridisation — a practical approach, IRL Press, pp 3–15

    Google Scholar 

  • Brysch W, Hagendorff G, Schlingensiepen (1988) RNA probes transcribed from synthetic DNA for in situ hybridisation. Nucleic Acids Res 16: 2333

    PubMed  CAS  Google Scholar 

  • Burns J, Graham AK, McGee J O’D (1988) Non isotopic detection of in situ nucleic acid in cervix: an updated protocol. J Clin Pathol 41: 897–899

    PubMed  CAS  Google Scholar 

  • Butler ET, Chamberlain MJ (1982) Bacteriophage SP6-specific RNA polymerase. J Biol Chem 257: 5772–5778

    PubMed  CAS  Google Scholar 

  • Caruthers MH, Beaucage SL, Efcavitch JW et al. (1982) Chemical Synthesis and biological studies on mutated gene control regions. Cold Spring Harbor Symp Quant Biol 47: 411–418

    CAS  Google Scholar 

  • Casey J, Davidson N (1977) Rates of formation and thermal stabilities of RNA: RNA and DNA: DNA duplexes at high concentrations of formamide. Nucleic Acids Res 4: 1539–1552

    PubMed  CAS  Google Scholar 

  • Chan VTW, McGee J (1987) Cellular oncogenes in neoplasia. J Clin Pathol 40: 1055–1063

    PubMed  CAS  Google Scholar 

  • Childs GV, Lloyd JM, Unabia G, Ghanb SD, Wierman ME, Chin WW (1987) Detection of luteinising hormone βmessenger ribonucleic acid (RNA) in individual gonadotropes after castration: use of a new in situ hybridisation method with a photobiotinylated complemen¬tary RNA probe. Molec Endocrinol 1: 926–932

    CAS  Google Scholar 

  • Chin-Yang L, Zeismer SC, Lazcano-Villareal O (1987) Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in immunoperoxidase method. J Histochem Cytochem 35: 1457–1460

    Google Scholar 

  • Cleveland DW, Lopata MA, MacDonald RJ, Cowan NJ, Rutter WJ, Kirschner MW (1980) Number and evolutionary conservation of a- and (3-tubulin and cytoplasmic P and y-actin genes using specific cloned cDNA probes. Cell 20: 95–105

    PubMed  CAS  Google Scholar 

  • Coggi G, Dell’Orto P, Viale G (1986) Avidin-biotin methods. In: Polak JM, van Noorden S (eds) Immunocytochemistry — modern methods and applications. Wright, Bristol, pp 54–70

    Google Scholar 

  • Coghlan JP, Aldred P, Haralambidis J, Niall HD, Penschow JD,Tregear GW(1985) Hybridisation histochemistry. Anal Biochem 149: 1–28

    Google Scholar 

  • Cox KH, De Leon DV, Angerer LM, Angerer RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridisation using asymmetric RNA probes. Dev Biol 101: 485–502

    PubMed  CAS  Google Scholar 

  • Cubie HA, Norval M (1988) Synthetic oligonucleotide probes for the detection of human papilloma viruses by in situ hybridisation. J Virol Methods 20: 239–249

    PubMed  CAS  Google Scholar 

  • Cuello AC (1983) Immunocytochemistry. J.Wiley, Chichester

    Google Scholar 

  • Cumming RDF, Fallon RA (1989) Subcellular localisation of biological molecules. In: Slater R (ed) Radioisotopes in Biology. IRL Press

    Google Scholar 

  • Davanloo P, Rosenberg AH, Dunn JJ, Studier FW (1984) Cloning and expression of the gene for bacteriophage 77 RNA polymerase. Proc Natl Acad Sci USA 81: 2035–2039

    PubMed  CAS  Google Scholar 

  • Edwards MK, Wood WB (1983) Location of specific messenger RNAs in Caenorhabditis elegans by cytological hybridisation. Dev Biol 97: 375–390

    PubMed  CAS  Google Scholar 

  • Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13

    PubMed  CAS  Google Scholar 

  • Feinberg AP, Vogelstein B (1984) A technique for radiolabelling DNA restriction endonuclease fragments to high specific acitivity. Addendum, Anal Biochem 137: 266–267

    PubMed  CAS  Google Scholar 

  • Fleming K (1987) In situ hybridisation - a role in clinical pathology. J Pathol 153: 201–202

    PubMed  CAS  Google Scholar 

  • Forhani B, Dupuis KW, Schmidt NJ (1985) Rapid detection of herpes simplex virus DNA in human brain tissue by in situ hybridisation. J Clin Microbiol 22: 656–658

    Google Scholar 

  • Forster AC, Mclnnes JL, Skingle DC, Symous RH (1985) Non-radioactive hybridisation probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photo- biotin. Nucleic Acids Res 13: 745–761

    PubMed  CAS  Google Scholar 

  • Fremeau RT, Lundblad JR, Pritchett DB, Wilcox JN, Roberts JL (1986) Regulation of proopiomelanocortin gene transcription in individual cell nuclei. Science 234: 1265–1269

    PubMed  CAS  Google Scholar 

  • Gall G, Pardue HL (1969) Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci USA 63: 378–381

    PubMed  CAS  Google Scholar 

  • Gastl G, Ward JH, Rapp UR (1986) Immunocytochemistry of oncogenes. In: Polak JM, Van Noorden S (eds) Immunocytochemistry — modern methods and applications. Wright, Bristol, pp 273–283

    Google Scholar 

  • Gee CE, Roberts JL (1983) A technique for the study of gene expression in single cells. DNA 2: 157 - 163

    PubMed  CAS  Google Scholar 

  • Gillam IC (1987) Non-radioactive probes for specific DNA sequences. Tib Tech 5: 332–334

    CAS  Google Scholar 

  • Gissman L, Schwarz E (1985) Cloning of papillomavirus DNA In: Becker Y(ed) Recombinant DNA research and viruses, Martinus Nijhoff, Boston, p 173

    Google Scholar 

  • Godard CM (1983) Improved method for detection of cellular transcripts by in situ hybridisation: detection of virus specific RNA in Rous sarcoma virus-infected cells by in situ hybridisation to cDNA. Histochemistry 77: 123–131

    PubMed  CAS  Google Scholar 

  • Goedert M (1986) Single-stranded DNA probes using an M 13 template In: Uhl G (ed) In situ hybridisation in brain. Plenum, New York, pp 236–237

    Google Scholar 

  • Goedert M, Fine A, Hunt SP, Ullrich A (1986) NGF-mRNA in peripheral and central rat tissue and in the human central nervous system: lesions effects in the rat brain and levels in Alzheimer’s disease. Mol Brain Res 1: 85–92

    Google Scholar 

  • Gonzalez RG, Blackburn BJ, Schleich T (1979) Fractionation and structural elucidation of the active components of aurintricarboxylic acid, a potent inhibitor of protein nucleic acid interactions. Biochim Biophys Acta 562: 534–545

    PubMed  CAS  Google Scholar 

  • Gowans EJ, Burrell CJ, Jilbert AR, Marmion BP (1981) Detection of hepatitis B virus sequencing in infected hepatocytes by in situ cytohybridisation. J Med Virol 8: 67–78

    PubMed  CAS  Google Scholar 

  • Gowans EJ, Burrell CJ, Jilbert AR, Marmion BP (1983) Patterns of single- and double- stranded hepatitis B virus DNA and viral antigen accumulation in infected liver cells. J Gen Virol 64: 1229–1239

    PubMed  Google Scholar 

  • Gowans EJ, Jilbert AR, Burrell CJ (1989) Detection of specific DNA and RNA sequences in tissues and cells by in situ hybridisation. CRC series, in press

    Google Scholar 

  • Grady-Leopardi EF, Schwab M, Ablin AR, Rosenau W (1986) Detection of N-myc oncogene expression in human neuroblastoma by in situ hybridisation and blot analysis relationship to clinical outcome. Cancer Res 46: 3196–3199

    PubMed  CAS  Google Scholar 

  • Guitteny AF, Fouque B, Mongin C,Teoule R, Boch B (1988) Histological detection of mRNAs with biotinylated synthetic oligonucleotide probes. J Histochem Cytochem 36: 563–571

    PubMed  CAS  Google Scholar 

  • Gupta JW, Gupta PK, Rosenshein N, Shah K (1987) Detection of human papillomavirus in cervical smears. A comparison of in situ hybridisation, immunocytochemistry and cytopathology. Acta Cytol 31: 387–396

    PubMed  CAS  Google Scholar 

  • Haase AT (1986) Analysis of viral infections by in situ hybridisation. J Histochem Cytochem 34: 27–32

    PubMed  CAS  Google Scholar 

  • Haase AT, Stowring LS, Narayan O, Griffin D, Price D (1977) Slow persistent infection caused by visna virus: role of host restriction. Science 195: 175–177

    PubMed  CAS  Google Scholar 

  • Haase AT, Stowring L, Harris JD, Traynor B, Ventura P, Peluso R, Brahic M (1982) Visna DNA synthesis and the tempo of infection in vitro. Virology 119: 399–410

    PubMed  CAS  Google Scholar 

  • Haase AT, Brahic M, Stowring L (1984) Detection of viral nucleic acids by in situ hybridisation. In: Maramorosch K, Koprowski H (eds) Methods in virology, VII. Academic Press, New York, pp 189–226

    Google Scholar 

  • Haase AT, Walker D, Stowring L et al. (1985) Detection by hybridisation of viral infection of the human central nervous system. Ann NY Acad Sci 436: 103–108

    Google Scholar 

  • Hafen E, Levine M, Garber RL, Gehring WJ (1983) An improved in situ hybridisation method for the detection of cellular RNAs in Drosophila tissue sections and its application for localising transcripts of the homoeotic antennapedia gene complex. EMBO J 2: 617–623

    PubMed  CAS  Google Scholar 

  • Hamid Q, Wharton J,Terenghi G et al. (1987) Localisation of atrial natriuretic peptide mRNA and immunoreactivity in the rat heart and human atrial appendage. Proc Natl Acad Sci USA 84: 6760–6764

    PubMed  CAS  Google Scholar 

  • Hayashi S, Gillam IC, Delaney AB, Tener GM (1978) Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridisation with [125I]-labelled RNA. J Histochem Cytochem 26: 677–679

    PubMed  CAS  Google Scholar 

  • Höfler H (1987) What’s new in “in situ hybridization”. Pathol Res Pract 182: 421–430

    PubMed  Google Scholar 

  • Höfler H, Childers H, Montminy MR, Lechan RM, Goodman RH, Wolfe HJ (1986a) In situ hybridisation methods for the detection of somatostatin mRNA in tissue sections using antisense RNA probes. Histochem J 18: 597–604

    Google Scholar 

  • Höfler H, Childers H, Dayal Y et al. (1986b) Detection of neuroendocrine gene expression of tumour cells by combined in situ hybridisation and immunocytochemistry. Verh Dtsch Ges Pathol 70: 211–216

    PubMed  Google Scholar 

  • Höfler H, Childers H, Mobtaker H, Tischer AS, De Lellis RA, Wolfe HJ (1986c) Fos oncogene expression in a mouse medullary thyroid carcinoma cell line demonstrated by blot and in situ hybridisation. Lab Invest 54:15 A

    Google Scholar 

  • Höfler H, Putz B, Ruhri C, Wirnsberger G, Klimpfinger M, Smolle J (1987) Simultaneous localisation of calcitonin mRNA and peptide in a medullary thyroid carcinoma. Virchows Arch [Cell Pathol]54:144–151

    Google Scholar 

  • Hogan B, Costantini F, Lacy E (1986) Manipulating the mouse embryo: a laboratory manual. Cold Spring Harbor Laboratory

    Google Scholar 

  • Howley PM (1987) The role of papillomaviruses in human cancer 4. In: Devita VT Jr, Hellman S, Rosenberg SA (eds) Important advances in oncology. Lippincott, Philadelphia

    Google Scholar 

  • Hu N, Messing J (1982) The making of strand specific M 13 probes. Gene 17: 271–277

    PubMed  CAS  Google Scholar 

  • Huang WM, Gibson SJ, Facer P, Gu J, Polak JM (1983) Improved section adhesion for immunocytochemistry using high molecular weight polymers of L-lysine as a slide coating. Histochemistry 77: 275–279

    PubMed  CAS  Google Scholar 

  • Hutchison NJ, Langer-Safer PR, Ward DC, Manikalo BA (1982) In situ hybridisation at the electron microscopic level: hybrid detection by autoradiography and colloidal gold. J Cell Biol 95: 609–618

    PubMed  CAS  Google Scholar 

  • Isenberg I (1979) Histones. Ann Rev Biochem 48: 159–191

    PubMed  CAS  Google Scholar 

  • Janssen HP, van Loon AM, Meddens MJ, Herbrink P, Linderman J, Quint WGV (1987) Comparison of in situ DNA hybridisation and immunological staining with conventional virus isolation for the detection of human cytomegolavirus infection in cell cultures. J Virol Methods 17: 311–318

    PubMed  CAS  Google Scholar 

  • Jilbert AR, Burrel CJ, Gowans EJ, Rowland R (1986) Histological aspects of in situ hybridisation. Histochemistry 85: 505–514

    PubMed  CAS  Google Scholar 

  • John HA, Burnstiel ML, Jones KW(1969) RNA:DNA hybrids at the cytological level. Nature 223: 582–587

    PubMed  CAS  Google Scholar 

  • Johnson SA, Morgan DG, Finch CE (1986) Extensive postmortem stability of RNA from rat and human brain. J Neurosci Res 16: 267–280

    PubMed  CAS  Google Scholar 

  • Jones TR, Hyman RW (1983) Specious hybridisation between herpes simplex virus DNA and human cellular DNA. Virology 131: 555–560

    PubMed  CAS  Google Scholar 

  • Kourilsky P, Leidner J, Tremblay GY (1971) DNA: DNA hybridisation, on filters at low temperature in the presence of formamide or urea. Biochimie 53: 1111–1114

    PubMed  CAS  Google Scholar 

  • Kwok S, Mack DH, Mullis KB et al. (1987) Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection. J Virol 61: 1690–1694

    PubMed  CAS  Google Scholar 

  • Langer PR, Waldrop A, Ward D (1981) Enzymatic synthesis of biotin labelled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78: 6633–6637

    PubMed  CAS  Google Scholar 

  • Larsson L-I, Christensen T, Dalboge H (1988) Detection of POMC mRNAby in situ hybridisation using a biotinylated oligodeoxynucleotide probe and avidin-alkaiine phosphatase histochemistry. Histochemistry 89: 109–116

    PubMed  CAS  Google Scholar 

  • Lawrence JB, Singer RH (1985) Quantitative analysis of in situ hybridisation methods for the detection of actin gene expression. Nucleic Acids Res 13: 1777–1799

    PubMed  CAS  Google Scholar 

  • Lawrence JB, Singer RH (1986) Intracellular localisation of mRNA for cytoskeletal proteins. Cell 45: 407–415

    PubMed  CAS  Google Scholar 

  • Lawrence JB, Villnave CA, Singer RH (1988) Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBVin a lymphoma line. Cell 52: 51–61

    PubMed  CAS  Google Scholar 

  • Leary JJ, Brigati DJ, Ward DC (1983) Rapid and sensitive colorimetric method for visualizing biotin-labelled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose. Proc Natl Acad Sci USA 80: 4045–4049

    PubMed  CAS  Google Scholar 

  • Levine BJ, Liv T, Marzluff WF, Skoultchi AI (1988) Differential expression of individual members of the histone multigene family due to sequences in the 5’ and 3’ regions of the genes. Mol Cell Biol 8: 1887–1895

    PubMed  CAS  Google Scholar 

  • Lewin BM (1980) Eukaryotic genomes. In: Gene expression 2:503–569, 861–930, Wiley, New York

    Google Scholar 

  • Lewis ME, Sherman TG, Watson SJ (1985) In situ hybridisation histochemistry with synthetic oligonucleotides; strategies and methods Peptides 6 [Suppl 2]: 75–87

    PubMed  CAS  Google Scholar 

  • Lewis ME, Arentzen R, Baldino F (1986) Rapid, high-resolution in situ hybridisation. Histochemistry with radioiodinated synthetic oligonucleotides. J Neurosci Res 16: 117–124

    PubMed  CAS  Google Scholar 

  • Lewis FA, Griffith S, Dunncliff R, Wells M, Dudding N, Bird CC (1987) Sensitive in situ hybridisation technique using biotin-streptavidin-polyalkaline phosphatase complex. J Clin Pathol 40: 163–166

    PubMed  CAS  Google Scholar 

  • Luehrsen KR, Baum MP (1987) In vitro synthesis of biotinylated RNA probes from A-Trich\ templates: problems and solutions. Biotechniques 5: 660–670

    CAS  Google Scholar 

  • Lynn DA, Angerer LM, Bruskin AM, Kleen WH, Angerer RC (1983): Localisation of a family of mRNAs in a single cell type and its precursors in sea urchin embryos. Proc Natl Acad Sci USA 80: 2656–2660

    PubMed  CAS  Google Scholar 

  • Maitland NJ, Cox MF, Lynas C, Prime S, Crane I, Scully C (1986) Nucleic acid probes in the study of latent viral disease. J Oral Pathol 16: 199–211

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory

    Google Scholar 

  • Manuelidis L (1985) Indications of centromere movement during interphase and differentiation. Ann NY Acad Sci 450: 205–221

    PubMed  CAS  Google Scholar 

  • McAllister LB, Scheller RH, Kaudel ER, Axel R (1983) In situ hybridisation to study the origin and fate of identified neurones. Science 222: 800–808

    PubMed  CAS  Google Scholar 

  • McAllister HA, Rock DL (1985) Comparative usefulness of tissue fixatives for in situ viral nucleic acid hybridisation. J Histochem Cytochem 33: 1026–1032

    PubMed  CAS  Google Scholar 

  • McConaughy BL, Laird CD, McCarthy BJ (1969) Nucleic acid reassociation in formamide. Biochemistry 8: 3289–3295

    PubMed  CAS  Google Scholar 

  • McDougall JK, Myerson D, Beckman AM (1986) Detection of viral DNA and RNA by in situ hybridisation. J Histochem Cytochem 34: 33–38

    PubMed  CAS  Google Scholar 

  • McKeating JA, Al-Nakib W, Greenaway PJ, Griffiths PD (1985) Detection of cytomegalovirus by DNA-DNA hybridisation employing probes labelled with 32P or biotin. J Virol Methods 11: 207–216

    PubMed  CAS  Google Scholar 

  • Meinkoth J, Wahl G (1984) Hybridisation of nucleic acids immobilized on solid supports. Anal Biochem 138: 267–284

    PubMed  CAS  Google Scholar 

  • Melton D, Kneg P, Rebagliati M, Maniatis T, Zinn K, Green MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridisation probes from plasmids containing a bacteriophage SP 6 promoter. Nucleic Acids Res 12: 7035–7056

    PubMed  CAS  Google Scholar 

  • Mitchell AR, Ambros P, Gosden JR, Morten JEN, Porteous DJ (1986) Gene mapping and physical arrangements of human chromatin in transformed hybrid cells: fluorescent and autoradiographic in situ hybridisation compared. Somatic Cell Mol Genet 12: 313–324

    CAS  Google Scholar 

  • Moench TR, Gendelman HE, Clements JE, Narayan O, Griffin DE (1985) Efficiency of in situ hybridisation as a function of probe size and fixation technique. J Virol Methods 11: 119–130

    PubMed  CAS  Google Scholar 

  • Morley DJ, Hodes ME (1988) Amylase expression in human parotid neoplasms: evidence by in situ hybridisation for lack of transcription of the amylase gene. J Histochem Cytochem 36: 487–492

    PubMed  CAS  Google Scholar 

  • Nan MM,Brooks BJ, Carney DN, Gazdar AF (1986) Human small cell lung cancers shows amplification and expression of the N-myc gene. Proc Natl Acad Sci USA 83: 1092–1096

    Google Scholar 

  • Naoumov NV, Alexander GJM, Eddieston ALWF, Williams R (1988) In situ hybridisation in formalin fixed, paraffin wax embedded liver specimens: method for detecting human and viral DNA using biotinylated probes. J Clin Pathol 41: 793–798

    PubMed  CAS  Google Scholar 

  • Niedobitek G, Finn HH, Bornhoft G, Gerdes J, Stein H (1988) Detection of viral DNA by in situ hybridisation using bromodeoxyuridine labelled DNA pfobes. Am J Pathol 1313: 1–4

    Google Scholar 

  • Palmer L, Falkow S (1985) Selection of DNA probes for use in the diagnosis of infectious disease. In: Kingsbury DT, Falkow S (eds) Rapid detection and identification of infectious diseases. Harcourt Brace Jovanovich, New York, pp 211–233

    Google Scholar 

  • Patient R (1984) DNA hybridisation - beware. Nature 308: 15–16

    PubMed  CAS  Google Scholar 

  • Penschow JD, Haralambidis J, Aldred P, Tregear GW, Coghlan JP (1986) Location of gene expression in CNS using hybridisation histochemistry. Methods Enzymol 124: 534–548

    PubMed  CAS  Google Scholar 

  • Polak JM, van Noorden SV(1986) Immunocytochemistry — modern methods and applications. Wright, Bristol

    Google Scholar 

  • Pontecorvi A, Tata JR, Phyllaier M, Robbins J (1988) Selective degradation of mRNA: the role of short-lived proteins in differential destabilization of insulin-induced creative Phosphokinase and myosin heavy chain mRNAs during rat skeletal muscles L6 cell differentiation. EMBO J 7: 1489–1495

    PubMed  CAS  Google Scholar 

  • Przepiorka D, Myerson D (1986) A single step silver enhancement method permitting rapid diagnosis of cytomegalovirus infection in formalin-fixed paraffin embedded tissue sections by in situ hybridisation and immunoperoxidase detection. J Histochem Cytochem 34: 1731–1734

    PubMed  CAS  Google Scholar 

  • Ralston R, Bishop JM (1984) Evolutionary relationships among oncogenes of DNA and RNA tumour viruses: myc, myb and adenovirus EI A In: Vande Woude GF, Levine AJ, Topp WC, Watson JD (eds) Cancer and cells 2: oncogenes and viral genes. Cold Spring Harbor Laboratory, pp 165–172

    Google Scholar 

  • Ranki A, Krohn M, Allain J et al. (1987) Long latency precedes overt seroconversion in sexually transmitted human-immunodeficiency-virus infection. Lancet II: 589–593

    Google Scholar 

  • Renz M, Kurz C (1984) A colorimetric method for DNA hybridisation. Nucleic Acids Res 12: 3435–3444

    PubMed  CAS  Google Scholar 

  • Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxyribonuclei acid to high specific activity in vitro by nick translation with DNA polymerase 1. J Mol Biol 113: 237–251

    PubMed  CAS  Google Scholar 

  • Rogers AW (1979) Techniques of autoradiography. Elsevier, Amsterdam

    Google Scholar 

  • Rogers WT, Schwaber JS, Lewis ME (1987) Quantitation of cellular resolution in situ hybridisation histochemistry in brain by image analysis. Neurosci Lett 82: 315–320

    PubMed  CAS  Google Scholar 

  • Ruda MA, Iadarola MJ, Cohen LV, Young III WS (1988) In situ hybridisation histochemistry and immunocytochemistry reveal an increase in spinal dynorphyn biosynthesis in a rat model of peripheral inflammation and hyperalgesia. Proc Natl Acad Sci USA 85: 622–626

    PubMed  CAS  Google Scholar 

  • Sacki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of (3-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anaemia. Science 230: 1350–1354

    Google Scholar 

  • Schwab M, Ellison J, Busch M, Rosenau W, Varmus HE, Bishop JM (1984) Enhanced expression of the human gene N-myc consequent to amplification of DNA may contribute to malignant progression of neuroblastoma. Proc Natl Acad Sci USA 81: 4940–4944

    PubMed  CAS  Google Scholar 

  • Scott Young III W, Mezey E, Siegel RE (1986) Vasopressin and oxytocin mRNAs in adrenalectomized and Brattleboro rats: analysis by quantitative in situ hybridisation histochemistry. Mol Brain Res 1: 231–241

    Google Scholar 

  • Shaw G, Kamen R (1986) A conserved AU sequence from the 3’ untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell 46: 659–667

    PubMed  CAS  Google Scholar 

  • Shivers BD, Harlan RE, Pfaff DW, Schachler BS (1986a) Combination of immunocytochemistry and in situ hybridisation in the same tissue section of rat pituitary. J Histochem Cytochem 34: 39–43

    PubMed  CAS  Google Scholar 

  • Shivers BD, Schachter BS, Pfaff DW (1986b) In situ hybridisation for the study of gene expression in the brain. Methods Enzymol 124: 497–510

    PubMed  CAS  Google Scholar 

  • Singer RH, Ward DC (1982) Actin gene expression visualized in chicken muscle culture by using in situ hybridisation with a biotinated nucleotide analog. Proc Natl Acid Sci 79: 7331–7335

    CAS  Google Scholar 

  • Singer RH, Lawrence JB, Villnave C (1986) Optimization of in situ hybridisation using isotopic and non-isotopic detection methods. Biotechniques 4: 230–250

    CAS  Google Scholar 

  • Slamon DC, de Kernion JB, Verma IH, Cline H (1984) Expression of cellular oncogenes in human malignancies. Science 224: 256–262

    Google Scholar 

  • Soreq H, Zamur R, Zevin-Sonkin D, Zakut H (1987) Human cholinesterase genes localized by hybridisation to chromosomes 3 and 16. Hum Genes 738: 1–4

    Google Scholar 

  • Stanssens P, Remaut E, FiersW(1986) Inefficient translation initiation causes premature transcription termination in the lac Z gene. Cell 44: 711–718

    Google Scholar 

  • Steel JH, Hamid Q, Van Noorden S et al. (1988) Combined use of in situ hybridisation and immunocytochemistry for the investigation of prolactin gene expression in immature, pubertal, pregnant, lactating and ovariectomised rats. Histochemistry 89: 75–80

    PubMed  CAS  Google Scholar 

  • Stopa EG, Uhl GR, Mobtaker H et al. (1989) Somatostatin gene expression in human brain: in situ hybridisation studies in postmortem tissue. Neuroscience, in press

    Google Scholar 

  • Stowring L, Haase AT, Petursson G et al. (1985) Detection of visna virus antigens and RNA in glial cells in foci in demyelination. Virology 141:311–318

    PubMed  CAS  Google Scholar 

  • Syrjänen S, Partanen P, Mantyjarvi R, Syrjanen K (1988) Sensitivity of in situ hybridisation techniques using biotin and 35S labelled human papilloma virus (HPV) DNA probes. Virol Methods 19: 225–238

    Google Scholar 

  • Szabo P, Elder R, Steffensen DM, Uhlenbeck OC (1977) Quantitative ISH of ribosomal RNA species to polytene chromosomes of Drosophila melanogaster. J Mol Biol 115: 539–563

    PubMed  CAS  Google Scholar 

  • Taylor GR, Carter GI, Crow TJ, Johnson JA, Fairbairn AF, Perry EK, Perry RH (1986) Recovery and measurement of specific RNA species from postmortem brain tissue: a general reduction in Alzheimer’s disease detected by molecular hybridisation. Exp Mol Pathol 44: 111–116

    PubMed  CAS  Google Scholar 

  • Terenghi G, Polak JM, Hamid Q et al. (1987) Localisation of neuropeptide Y mRN A in neurons of human cerebral cortex by means of in situ hybridisation with a complementary RNA probe. Proc Natl Acad Sci USA 84: 7315–7318

    PubMed  CAS  Google Scholar 

  • Thomas CA, Dancis BM (1973) Ring stability. J Mol Biol 77: 44–55

    Google Scholar 

  • Tournier I, Bernau D, Poliard A, Schoevaret D, Feldman G (1987) Detection of albumin mRNAs in rat liver by in situ hybridisation: usefulness of paraffin embedding, and comparison of various fixation procedures. J Histochem Cytochem 35: 453–459

    PubMed  CAS  Google Scholar 

  • Trask B, van den Engh G, Pinkel D, Mullikin J, Waldmann F, van Dekken H, Gray J (1988) Fluorescence in situ hybridisation to interphase cell nuclei in suspension allows flow cytometric analysis of chromosome content and microscopic analysis of nuclear organization. Hum Genet 78: 251–259

    PubMed  CAS  Google Scholar 

  • Unger ER, Budgeon LR, Myerson D, Brigati DJ (1986) Viral diagnosis by in situ hybridisation. Description of a rapid simplified colorimetric method. Am J Surg Pathol 10: 1–8

    PubMed  CAS  Google Scholar 

  • van der Ploeg, Landegent JE, Hopman HHN, Raap AK (1986) Non-autoradiographic hybridocytochemistry. J Histochem Cytochem 34: 126–133

    Google Scholar 

  • Wahl GM, Stern M, Stark GR (1979) Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridisation by using dextran sulphate. Proc Natl Acad Sci USA 76: 3683–3687

    PubMed  CAS  Google Scholar 

  • Wetmur JG, Davidson N (1968) Kinetics of renaturation of DNA. J Mol Biol 31: 349–370

    PubMed  CAS  Google Scholar 

  • Wetmur JG, Ruyechan WT, Douthart RJ (1981) Denaturation and renaturation of Penicillinin chrysogenum mycophage double-stranded ribonucleic acid in tetraalkylammonium salt solutions. Biochemistry 20: 2999–3002

    PubMed  CAS  Google Scholar 

  • Wilcox JN, Gee CE, Roberts JL (1986) In situ cDNA-mRNA hybridisation: development of a technique to measure mRNA levels in individual cells. Methods Enzymol 124: 510–533

    PubMed  CAS  Google Scholar 

  • Zabel M, Schafer H (1988) Localisation of calcitonin and calcitonin gene-related peptide mRNAs in rat parafollicular cells by hybridocytochemistry. J Histochem Cytochem 36: 543–546

    PubMed  CAS  Google Scholar 

  • Zajac JD, Penschow J, Mason T, Tregear G, Coghlan J, Martin TJ (1986) Identification of calcitonin and calcitonin gene-related peptide messenger ribonucleic acid in medullary thyroid carcinomas by hybridisation histochemistry. J Clin Endocrinol Metab 62: 1037–1043

    PubMed  CAS  Google Scholar 

Download references

Authors
  1. G. Terenghi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. A. Fallon
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Pathology, University of Sheffield Medical School, Beech Hill Road, S10 2RX, Sheffield, UK

    James C. E. Underwood Professor, M.D., F.R.C. Path.

Rights and permissions

Reprints and permissions

Copyright information

© 1990 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Terenghi, G., Fallon, R.A. (1990). Techniques and Applications of In Situ Hybridisation. In: Underwood, J.C.E. (eds) Pathology of the Nucleus. Current Topics in Pathology, vol 82. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74668-0_7

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-74668-0_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74670-3

  • Online ISBN: 978-3-642-74668-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation