Acute Monocytic Leukemia with Translocation t(1;11) (p31;q23):Simultaneous Staining of Chromosomes and Cell Surface Antigens

  • I. Nölle
  • B. Schlegelberger
  • N. Schmitz
  • S. Bödewadt-Radzun
  • W. Grote
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 33)

Abstract

Leukemia is thought to be the clonal expansion of a single mutated cell. Depending on the differentiation stage of this cell, different cell lineages might be involved in tumorigenesis. Keinänen et al. [2] have shown that myeloid, erythrocytic, and megakaryocytic lineages were involved in 8 out of 12 cases of acute myeloid leukemia with monosomy 7 or trisomy 8. In order to evaluate the lineage involvement in a case of acute monocytic leukemia with trisomy 8 and a translocation t(1;11), we modified a method described by Teerenhovi et al. [1]. With this method it is possible to analyze cell surface antigens and chromosomes of the same cell, whereas by standard chromosome preparation the cell membrane and cytoplasmic structures are destroyed.

Keywords

Acetone Leukemia Chloroform Stein Lution 

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References

  1. 1.
    Teerenhovi L, Knuutila S, Ekblom M, Rossi L, Borgström GH, Tallman JK, Anderson L, de la Chapelle (1984) A method for simultaneous study of the karyotype, morphology, and immunologic phenotype of mitotic cells in hematologic malignancies. Blood 64:1116–1122PubMedGoogle Scholar
  2. 2.
    Keinänen MM, Griffin J, Bloomfield CD, Machnicki J, de la Chapelle A (1988) Clonal chromosomal abnormalities showing multiple-cell-lineage involvement in acute myeloid leukemia. N Engl J Med 318:1153–1158PubMedCrossRefGoogle Scholar
  3. 3.
    Moorhead PS, Nowell PC, Mellman WJ, Ballips DM, Hungerford DA (1960) Chromosome preparations of leukocytes cultures from human peripheral blood. Exp Cell Res 20:613–616PubMedCrossRefGoogle Scholar
  4. 4.
    Casperson T, Zech L, Johansson C, Modest EJ (1970) Identification of human chromosomes by DNA-binding fluorescent agents. Chromosoma 30:215–227CrossRefGoogle Scholar
  5. 5.
    Sahar E, Latt SA (1978) Enhancement of binding patterns in human metaphase chromosomes by energy transfer. Proc Natl Acad Sci 75:5650–5654PubMedCrossRefGoogle Scholar
  6. 6.
    Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, MacDonald S, Pulford K, Stein H, Mason DY (1984) Immunenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase ( APAAP complexes ). J Histochem Cytochem 32:219–229PubMedCrossRefGoogle Scholar
  7. 7.
    Radzun HJ, Parwaresch MR (1983) Differential immunohistochemical resolution of the human mononuclear phagocyte system. Cell Imunol 82:174–183CrossRefGoogle Scholar
  8. 8.
    Radzun HJ, Parwaresch MR, Bode wadt S, Sundstrom C, Lennert K (1985) Bimodal differentiation prospectives for promyelocytes. JNCI 75:199–206PubMedGoogle Scholar
  9. 9.
    Parwaresch MR, Radzun HJ, Kreipe H, Hansmann HL, Barth J (1986) Monocyte/makrophage-reactive monoclonal antibody Ki-M6 recognizes an intracytoplasmic antigen. Am J Pathol 125:141–151PubMedGoogle Scholar
  10. 10.
    Hagemeijer A, Hählen K, Sizoo W, Abels J (1982) Translocation (9;11) (p21;q23) in three cases of acute monoblastic leukemia. Cancer Genet Cytogenet 5:95–105PubMedCrossRefGoogle Scholar
  11. 11.
    Kaneko Y, Maseki N, Takasaki N, Sakurai M, Hayashi Y, Nakazawa S, Mori T, Sakurai M, Takeda T, Hiyoshi Y (1986) Clinical and hematologic characteristics in acute leukemia with 11q23 translocations. Blood 67:484–491PubMedGoogle Scholar
  12. 12.
    Abé R, Sandberg AA (1984) Significance of abnormalities involving segment 1 lq22-25 in acute leukemia. Cancer Genet Cytogenet 13:121–127PubMedCrossRefGoogle Scholar
  13. 13.
    Pikler GM, Gay B, Stamper S (1986) Cytoge-netic findings in acute monocytic leukemia in a renal allograft recipient. Cancer Genet Cytogent 12:175–178Google Scholar
  14. 14.
    Caubet JF, Gegone A, Stehelin D, Berger R (1986) Localisation anormale du’une leucemie aiguë avec translocation t(1;11) (q21;q23). EMBO J 4:2245–2248Google Scholar
  15. 15.
    Berger R, Bernheim A, Flandrin G, Dresch C, Najean Y (1984) Cytogenetic studies on acute nonlymphocytic leukemias following polycythemia vera. Cancer Genet Cytogenet 11:441–451PubMedCrossRefGoogle Scholar
  16. 16.
    Sait J, Raza A, Sandberg A A (1987) A t(1;11) in acute nonlymphocytic leukemia FAB type M4. Cancer Genet Cytogenet 24:181–183PubMedCrossRefGoogle Scholar
  17. 17.
    Cuneo A, Barbieri D, Ferraresi P, Castoldi GL (1985) A case of chronic myelogenous leukemia with 11q- in blast crisis with monoblastic differentiation. Nouv Rev Fr Hematol 27:389–391PubMedGoogle Scholar
  18. 18.
    Esseltine DW, Vekemans M, Seemayer T, Race E, Gordon J, Whitehead VW (1982) Significance of a t(4;11) translocation in acute lymphoblastic leukemia. Cancer 50:503506Google Scholar
  19. 19.
    Third international workshop on chromosomes in leukemia (1980) Chromosomal abnormalities in acute lymphoblastic leukemia:structural and numerical changes of 234 cases. Cancer Genet Cytogenet 4:101–110Google Scholar
  20. 20.
    Williams DL, Look AT, Melvin SL, Roberson PK, Dahl G, Flake T, Stass S (1984) New chromosomal translocations correlate with specific immunophenotypes of childhood lymphoblastic leukemia. Cell 36:101–106PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • I. Nölle
    • 1
  • B. Schlegelberger
    • 1
  • N. Schmitz
    • 2
  • S. Bödewadt-Radzun
    • 3
  • W. Grote
    • 1
  1. 1.Dept. of Human GeneticsUniversity, KielGermany
  2. 2.Dept. of Internal MedicineUniversity, KielGermany
  3. 3.Department of PathologyUniversity, KielGermany

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