Limited Proliferative Potential of Primitive Hematopoietic Stem Cells: Hematopoiesis by Clonal Succession

  • J. L. Chertkov
  • E. I. Deryugina
  • N. J. Drize
  • E. Yu. Sadovnikova
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 32)


The hierarchy of hematopoietic stem cells (HSC) is represented by several categories of maturating pluripotent pro-genitors. Most of them are the members of transitional cell populations and, obviously, have no capacity for self-maintenance, i.e., are not capable of giving rise to self-replicating offspring with the same proliferative potential as the parent had [1]. The foundator of this hierarchy has not yet been identified. The most probable candidate at present is the cells supporting long-term hematopoiesis in vivo after repopulation of lethally irradiated or genetically defective W-mutant mice or in vitro in long-term culture. However, the self-renewal is also not proven for these cells, and hematopoiesis, at least in culture, occurs by clonal succession [2]. The experimental data support the hypothesis that even primitive HSC (PHSC) exhibit high, though limited, proliferative potential. This cell category is usually identified by competitive repopulation assay using a mixture of tested and standard cells identifiable by biochemical, immunological, karyological, or other markers [3]. Limiting dilution analysis based on the ability of small numbers of +/+ hematopoietic cells to cure anemia of W-mutant mice has been also used for the determination of PHSC [4–6].


Bone Marrow Cell Murine Bone Marrow Stromal Precursor Limit Dilution Method Limit Dilution Analysis 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • J. L. Chertkov
    • 1
  • E. I. Deryugina
    • 1
  • N. J. Drize
    • 1
  • E. Yu. Sadovnikova
    • 1
  1. 1.Hematological Scientific CenterMoscowU.S.S.R.

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