Coupling Chromatographic Techniques with FABMS for the Structural Analysis of Biological Compounds

  • Richard M. Caprioli
Conference paper

Abstract

High performance liquid chromatography (HPLC) has become an indispensable tool to the biochemist and molecular biologist for the separation of polar and charged compounds derived from biological systems. It is obvious that coupling this technique to mass spectrometry provides a powerful mass specific detection and analysis system which is unmatched in terms of its combined specificity and sensitivity. The success of FAB for the analysis of polar compounds has led to a number of approaches for coupling HPLC and FABMS. This article will discuss interfacing the continuous-flow fast atom bombardment (CF-FAB) technique with HPLC (1, 2) and applications of this technique. It is noted that other methods of coupling these have been described, namely, the moving belt (3) and frit-FAB techniques (4). Other types of separation processes have been coupled with FAB as well, including thin layer chromatography (TLC) (5) and capillary zone electrophoresis (CZE) (6). All three separation techniques play significant roles in the biochemistry laboratory, and are significantly enhanced when coupled with mass spectrometry.

Keywords

High Performance Liquid Chromatography Adduct Polypeptide Trypsin Disulfide 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Caprioli RM, DaGue B, Fan T, Moore WT (1987) BBRC 146: 291PubMedGoogle Scholar
  2. 2.
    Caprioli RM, DaGue B, Wilson K (1988) J Chromatogr Sci (in press)Google Scholar
  3. 3.
    Stroh JG, Cook CC, Milberg RM, Brayton L, Kihara T, Huang Z, Rhinehart KL (1985) Anal Chem 57: 985CrossRefGoogle Scholar
  4. 4.
    Ito Y, Takeuchi T, Ishii D, Goto M (1985) J Chromatogr 346: 161CrossRefGoogle Scholar
  5. 5.
    Shizukuishi K, Numaziri Y, Kato Y (1986) Proceedings Symp Med Res 11: 85Google Scholar
  6. 6.
    Smith RD, Olivares JA, Nguyen NT, Udseth HR (1988) Anal Chem 60: 436CrossRefGoogle Scholar
  7. 7.
    Moore WT, DaGue B, Martin M, Caprioli RM (1988) Proceedings 36th ASMS Conf on Mass Spectrom, June 5–10, San Francisco 1081Google Scholar
  8. 8.
    Boulenguer P, Leroy Y, Alonso J, Montreuil J, Ricart G, Colbert C, Duquet D, Dewaele C, Fournet B (1988) Anal Bchm 168: 164CrossRefGoogle Scholar
  9. 9.
    Lisek CA, Bailey J, Aimone LD, Benson L, Yaksh TL, Jardine I (1988) Proceedings 36th ASMS Conf on Mass Spectrom, June 5–10, San Francisco 1083Google Scholar
  10. 10.
    Minard RD, Chin-Fat D, Curry P, Ewing AG (1988) Proceedings 36th ASMS Conf on Mass Spectrom, June 5–10, San Francisco 950Google Scholar
  11. 11.
    Nelson R, Vourous PV, Karger BL, unpublished dataGoogle Scholar
  12. 12.
    Chang TT, Lay JO, Francel RJ (1984) Anal Chem 56: 109CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Richard M. Caprioli
    • 1
  1. 1.The Analytical Chemistry Center and Department of Biochemistry and Molecular BiologyThe University of Texas Medical School at HoustonHoustonUSA

Personalised recommendations