Metabolic Stabilization of p53 in SV40-Transformed Cells Correlates with Expression of the Transformed Phenotype but is Independent from Complex Formation with SV40 Large T Antigen

  • W. Deppert
  • T. Steinmayer
Conference paper
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 144)

Abstract

One of the most prominent changes in cellular protein expression observed after transformation with SV40 is the drastically enhanced level of the cellular protein p53 (reviewed in Crawford 1983). This protein is involved in the control of cell proliferation and also has a demonstrated oncogenic potential (reviewed in Oren 1985; Rotter and Wolf 1985). This p53 is rapidly turned over in normal cells but becomes metabolically stabilized in SV40-transformed cells. Since p53 in SV40-transformed cells forms a tight complex with the large T antigen, it has long been assumed that in these cells metabolic stabilization of p53 is the result of the physical interaction of these proteins (reviewed in Crawford 1983). However, since SV40-transformed cells, in addition to p53 complexed to large T antigen, also harbor free (i.e., noncomplexed) p53 which is metabolically stable (Deppert and Haug 1986; Deppert et al. 1987), alternative mechanisms for metabolic stabilization of p53 have to be envisioned.

Keywords

Fractionation 

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References

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Copyright information

© Springer-Verlag Berlin · Heidelberg 1989

Authors and Affiliations

  • W. Deppert
    • 1
  • T. Steinmayer
    • 2
  1. 1.Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität HamburgGermany
  2. 2.Abteilung Biochemie der Universität UlmUlmGermany

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