Analysis of Hepatitis B Virus Gene Functions in Tissue Culture and In Vivo

  • H.-J. Schlicht
  • H. Schaller
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 144)


The hepatitis B viruses, also called hepadnaviruses, are enveloped DNA viruses which primarily infect liver cells (reviewed by Ganem and Varmus 1987). The most important member of this virus family is the human hepatitis B virus (HBV; Dane et al. 1970), which is the causative agent of a severe form of hepatitis in humans. Since the virus is preferentially transmitted by contact with infected blood or blood products, this form of hepatitis was called serum hepatitis or hepatitis B in contrast to hepatitis A, which is caused by a Picornavirus typically transmitted by contaminated food.


Human Hepatoma Cell Line Minus Strand Terminal Protein Viral Core Particle Core Gene Product 
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  1. Acs G, Sells M, Purcell R, Price P, Engle R, Shapiro M, Popper H (1987) Hepatitis B virus produced by transfected Hep G2 cells causes hepatitis in chimpanzees. Proc Natl Acad Sci USA 84: 4641–4644PubMedCrossRefGoogle Scholar
  2. Babinet C, Farza H, Morello D, Hadchouel M, Pourcel C (1985) Specific expression of hepatitis B surface antigen (HBsAg) in transgenic mice. Science 230: 1160–1163PubMedCrossRefGoogle Scholar
  3. Bartenschlager R, Schaller H (1988) The amino terminal domain of the hepadnaviral P-gene encodes the terminal protein (genome linked protein) believed to prime reverse transcription The EMBO Journal 7: 4185–4192.PubMedGoogle Scholar
  4. Chisari F, Pinkert C, Milich D, Filipi P, McLachlan A, Palmiter R, Brinster R (1985) A transgenic mouse model of the chronic hepatitis B surface antigen carrier state. Science 230: 1157–1160PubMedCrossRefGoogle Scholar
  5. Chang C, Jeng K, Hu C, Lo S, Su T, Ting L, Chou C, Han S, Pfaff E, Salfeld J, Schaller H (1987) Production of hepatitis B virus in vitro by transient expression of cloned HBV DNA in a hepatoma cell line. EMBO J. 6: 675–680PubMedGoogle Scholar
  6. Dane D, Cameron C, Biggs M (1970) Virus like particles in serum of patients with Australia antigen associated hepatitis. Lancet 1: 695–698PubMedCrossRefGoogle Scholar
  7. Galle P, Schlicht HJ, Fischer M, Schaller H (1988) Production of infectious duck hepatitis B virus in a human hepatoma cell line. J Virol 62: 1736–1740PubMedGoogle Scholar
  8. Ganem D, Varmus H (1987) The molecular biology of the hepatitis-B viruses. Ann. Rev. Biochem. 56: 651–693PubMedCrossRefGoogle Scholar
  9. Hoofnagle J, Shafritz D, Popper H (1987) Chronic type B hepatitis and the “healthy” HBsAg carrier state. Hepatology 7: 758–763PubMedCrossRefGoogle Scholar
  10. Junker M, Galle P, Schaller H (1987) Expression and replication of the hepatitis-B virus genome under foreign promoter control Nucleic Acids Res 15: 10117–10132PubMedCrossRefGoogle Scholar
  11. Linial M (1987) Creation of a processed pseudogene by retroviral infection. Cell 49: 93–102PubMedCrossRefGoogle Scholar
  12. Nagaya T, Nakamura T, Tokino T, Tsurimoto T, Imai M, Mayumi T, Kamino K, Yamamura K, Matsubara K (1987) The mode of hepatitis B virus DNA integration in chromosomes of human hepatocellular carcinoma. Genes and Development 1: 773–782PubMedCrossRefGoogle Scholar
  13. Popper H, Shafritz D, Hoofnagle J (1987 a) Relation of the hepatitis B virus carrier state to hepatocellular carcinoma. Hepatology 7: 764–772PubMedCrossRefGoogle Scholar
  14. Popper H, Roth L, Purcell R, Tennant B, Gerin J (1987 b) Hepatocarcinogenicity of the wood-chuck hepatitis virus. Proc Natl Acad Sci USA 84: 866–870PubMedCrossRefGoogle Scholar
  15. Schlicht HJ, Galle P, Schaller H (1987 a) The hepatitis B viruses: molecular biology and recent tissue culture systems J Cell Sci [Suppl] 7: 197–212Google Scholar
  16. Schlicht HJ, Salfeld J, Schaller H (1987 b) The pre-C region of the duck hepatitis-B virus is essential for synthesis and secretion of processed core proteins but not for virus formation. J Virol 61: 3701–3709PubMedGoogle Scholar
  17. Schlicht HJ, Radziwill G, Schaller H (1989) Synthesis and encapsidation of duck hepatitis B virus reverse transcriptase do not require formation of core polymerase fusion proteins. Cell 56: 85–92PubMedCrossRefGoogle Scholar
  18. Spandau D, Lee C (1988) trans-Activation of viral enhancers by the hepatitis B virus X protein. J Virol 62: 427–434PubMedGoogle Scholar
  19. Sprengel R, Kuhn C, Will H, Schaller H (1985) Comparative sequence analysis of duck and human hepatitis-B virus genomes. J Med Virol 15: 323–333PubMedCrossRefGoogle Scholar
  20. Summers J, Smolec J, Snyder R (1978) A virus similar to human hepatitis B virus associated with hepatitis and hepatoma in woodchucks. Proc Natl Acad Sci USA 75: 4533–4537PubMedCrossRefGoogle Scholar
  21. Sureau C, Roup-Lemonne J, Mullins J, Essex M (1986) Production of hepatitis B virus by a differentiated human hepatoma cell line after transfection with cloned circular HBV DNA. Cell 47: 37–47PubMedCrossRefGoogle Scholar
  22. Tsurimoto T, Fujiyama A, Matsubara K (1987) Stable expression and replication of hepatitis B virus genome in an integrated state in a human hepatoma cell line transfected with the cloned viral DNA. Proc Natl Acad Sci USA 84: 444–448PubMedCrossRefGoogle Scholar
  23. Tuttleman J, Pugh J, Summers J (1986) In vitro experimental infection of primary duck hepatocyte cultures with duck hepatitis-B virus. J Virol 58: 17–25PubMedGoogle Scholar
  24. Will H, Cattaneo R, Koch H, Darai G, Schaller H, Schellekens P, van Eerd C, Deinhardt F (1982) Cloned HBV DNA causes hepatitis in chimpanzees. Nature (London) 299: 740–741CrossRefGoogle Scholar
  25. Will H, Cattaneo R, Darai G, Deinhardt F, Schellekens H, Schaller H (1985) Infectious hepatitis B virus from cloned DNA of known nucleotide sequence. Proc Natl Acad Sci USA 82: 891–895PubMedCrossRefGoogle Scholar
  26. Yaginuma K, Shirakata Y, Kobayashi M, Koike K (1987) Hepatitis B virus (HBV) particles are produced in a cell culture system by transient expression of transfected HBV DNA. Proc Natl Acad Sci USA 84: 2678–2682PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1989

Authors and Affiliations

  • H.-J. Schlicht
    • 1
  • H. Schaller
    • 1
  1. 1.Zentrum für Molekulare BiologieUniversität HeidelbergHeidelberg 1Germany

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