Toward Directed Modulation of Rosmarinic Acid Production in Cultured Plant Cells
Hydroxycinnamoyl conjugates form a class of metabolites whose production in cultured plant cells is often remarkably high (for review, see Ellis 1985). Questions of metabolic regulation can be posed in such high production systems which are very relevant to attempts to reduce or enhance the accumulations of specific metabolites in other culture or whole plant systems. Determination of which combination of regulatory mechanism(s), genetic organization and environmental cue(s) results in accumulation of such high concentration (as much as 25% of the tissue biomass) of a single metabolite should provide important insights for plant metabolic regulation in general. More specifically, the information may suggest new approaches for rational intervention in specific plant systems where modified expression of a metabolic phenotype is desired. The particular advantage of a high-producing culture system is that the enzyme catalysts, together with their associated short- and long-term regulatory processes, clearly are present and functional within a readily manipulated tissue.
KeywordsChlorogenic Acid Rosmarinic Acid Shikimic Acid Tyrosine Aminotransferase Rosmarinic Acid Production
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