Toward Directed Modulation of Rosmarinic Acid Production in Cultured Plant Cells

  • W. De Eknamkul
  • B. E. Ellis
Conference paper

Abstract

Hydroxycinnamoyl conjugates form a class of metabolites whose production in cultured plant cells is often remarkably high (for review, see Ellis 1985). Questions of metabolic regulation can be posed in such high production systems which are very relevant to attempts to reduce or enhance the accumulations of specific metabolites in other culture or whole plant systems. Determination of which combination of regulatory mechanism(s), genetic organization and environmental cue(s) results in accumulation of such high concentration (as much as 25% of the tissue biomass) of a single metabolite should provide important insights for plant metabolic regulation in general. More specifically, the information may suggest new approaches for rational intervention in specific plant systems where modified expression of a metabolic phenotype is desired. The particular advantage of a high-producing culture system is that the enzyme catalysts, together with their associated short- and long-term regulatory processes, clearly are present and functional within a readily manipulated tissue.

Keywords

Tyrosine Kanamycin Phenylalanine Sorghum Hygromycin 

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References

  1. Bonner CA, Jensen RA (1985) Novel features of prephenate aminotransferase from cell cultures of Nicotiana silvestris. Arch Biochem Biophys 238:237–246PubMedCrossRefGoogle Scholar
  2. Bolwell GP, Sap J, Cramer CL, Lamb CJ, Schuch W, Dixon RA (1986) L-phenylalamine ammonia-lyase from Phaseolus vulgaris: partial degradation of enzyme subunits in vitro and in vivo. Biochem Biophys Acta 881:210–221CrossRefGoogle Scholar
  3. D-Eknamkul W, Ellis BE (1984) Rosmarinic acid production and growth characteristics of Anchusa officinalis cell suspension cultures. Planta Med 51:346–350CrossRefGoogle Scholar
  4. D-Eknamkul W, Ellis BE (1987a) Tyrosine aminotransferase: the entry point enzyme of the tyro-sine-derived pathway in rosmarinic acid biosynthesis. Phytochemistry 26:1941–1946CrossRefGoogle Scholar
  5. D-Eknamkul W, Ellis BE (1987b) Purification and characterization of tyrosine aminotransferase activities from Anchusa officinalis cell cultures. Arch Biochem Biophys 257:430–438CrossRefGoogle Scholar
  6. D-Eknamkul W, Ellis BE (1988) Purification and characterization of prephenate aminotransferase from Anchusa officinalis cell cultures. Arch Biochem Biophys (in press)Google Scholar
  7. Ellis BE (1985) Metabolism of caffeoyl derivatives in plant cell cultures. In: Neuman K-H, Barz W, Reinhard E (eds) Primary and secondary metabolism of plant cell cultures. Springer, Berlin Heidelberg New York Tokyo, p 164CrossRefGoogle Scholar
  8. Ellis BE, Towers GHN (1970) Biogenesis of rosmarinic acid in Mentha. Biochem J 118:291–297PubMedGoogle Scholar
  9. Ellis BE, Remmen S, Goeree G (1979) Interactions between parallel pathways during biosynthesis of rosmarinic acid in cell suspension cultures of Coleus blumei. Planta 147:163–167CrossRefGoogle Scholar
  10. Hanson KR, Zucker M (1963) The biosynthesis of chlorogenic acid and related conjugates of the hydroxycinnamic acids. J Biol Chem 238:1105–1115PubMedGoogle Scholar
  11. Jensen RA (1986) Tyrosine and phenylalanine biosynthesis: relationship between alternative pathways, regulation and subcellular location. In: Conn EE (ed) Recent advances in phytochemistry, vol 20. The shikimic acid pathway. Plenum, New York London, p 57Google Scholar
  12. Kiihnl T, Koch U, Heller W, Wellmann E (1987) Chlorogenic acid biosynthesis: characterization of a light-induced microsomal 5-0-(4-coumaroyl)-D-quinate/shikimate 3′-hydroxylase from carrot (Daucus carota L.) cell suspension cultures. Arch Biochem Biophys 258:226–232CrossRefGoogle Scholar
  13. Siehl DL, Connelly JA, Conn EE (1986) Tyrosine biosynthesis in Sorghum bicolor: characteristics of prephenate aminotransferase. Z Naturforsch 41C: 79–86Google Scholar
  14. Vaughan PFT, Butt VS (1969) The hydroxylation of p-coumaric acid by an enzyme from leaves of spinach beet (Beta vulgaris L.) Biochem J 113:109–115PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • W. De Eknamkul
  • B. E. Ellis
    • 1
  1. 1.Department of Chemistry and BiochemistryUniversity of GuelphGuelphCanada

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