Biosynthesis of Tropane Alkaloids
As with other secondary products, the biosynthesis of tropane alkaloids in plants has been extensively investigated by the use of labelled precursors (for a review, see ). Recent advances in the techniques of alkaloid analysis and enzyme purification, however, have now made it possible to isolate and characterize biosynthetic enzymes in the pathway, and earlier results using precursor feeding must be validated and may necessitate a revision of the biosynthetic pathways proposed. A prerequisite to such studies is a sufficient supply of cell material with high biosynthetic capability, thus, with high enzyme activities.
KeywordsNicotine Alkaloid Succinate Deuterium Epoxidation
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- Endo T, Komiya T, Masumitsu Y, Morikawa M, Yamada Y (1987) An intergeneric hybrid cell line of Duboisia hopwoodii and Nicotiana tabacum by protoplast fusion. J Plant Physiol 129:453–459Google Scholar
- Endo T, Komiya T, Mino M, Nakanishi K, Fujita S, Yamada Y (1988) Genetic diversity among sublines originating from a single somatic hybrid cell of Duboisia hopwoodii + Nicotiana tabacum. Theor Appl Genet (in press)Google Scholar
- Hashimoto T, Yukimune Y, Yamada Y (1986) Tropane alkaloid production in Hyoscyamus root cultures. J Plant Physiol 124:61–75Google Scholar