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Nuclear Magnetic Resonance Spectroscopic Techniques for the Study of Cellular Function

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Advances in Comparative and Environmental Physiology

Part of the book series: Advances in Comparative and Environmental Physiology ((COMPARATIVE,volume 5))

Abstract

The tremendous strides in metabolic research that have taken place over the last 50 years have largely been accomplished through studies of subcellular systems. For instance, metabolite levels and enzyme activities have been determined in extracts of cells. Enzymes have been highly purified and kinetic properties analyzed in in vitro systems where substrate concentrations far exceed enzyme concentrations. All of these approaches involve physical disruption of cells resulting in relatively homogeneous extracts devoid of the ordered microstructure found in living cells.

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Authors and Affiliations

  1. Department of Biological Science, The Florida State University, Tallahassee, FL, 32306, USA

    Robert W. Wiseman

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  1. W. Ross Ellington
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  2. Robert W. Wiseman
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© 1989 Springer-Verlag Berlin Heidelberg

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Ellington, W.R., Wiseman, R.W. (1989). Nuclear Magnetic Resonance Spectroscopic Techniques for the Study of Cellular Function. In: Advances in Comparative and Environmental Physiology. Advances in Comparative and Environmental Physiology, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74510-2_4

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  • DOI: https://doi.org/10.1007/978-3-642-74510-2_4

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  • Print ISBN: 978-3-642-74512-6

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