Growth and Cell Proliferation of Human Renal Cell Carcinomas Xenografted into BALB/c nu/nu Mice
Since the first report of a human malignant tumor growing in a mouse mutant by Rygaard and Povlsen in 1969, the nude mouse model has been frequently used in studies of growth (Katsuoka et al. 1976; Shimosato et al. 1976; Giovanella et al. 1978; Auerbach et al. 1978; Kyriazis et al. 1978) and therapy (Otto et al. 1984a, b; Bubenik et al. 1987; Taetle et al. 1987; Byers et al. 1987) of human tumors. Although this model is much closer to the human tumor than experimental animal tumors, the question is how far the nude mouse model actually represents the conditions in human beings. Furthermore, investigations in this field are mainly based on measurements of the tumor size (Giovanella et al. 1978; Auerbach et al. 1978; Höehn and Schroeder 1978; Naito et al. 1987) as well as of the DNA content of the tumor cells and its distribution by flow cytometry (Otto et al. 1984a, b; Baisch et al. 1986) There are almost no studies on cell proliferation of human tumors in nude mice apart from occasional determinations of the labeling and the mitotic index (Heller et al. 1987). The aim of the present study was a quantitative analysis of the growth and proliferation of human renal cell carcinomas growing in the nude mouse.
KeywordsFormalin Adenocarcinoma Hematoxylin Eosin DAPI
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