Solute Translocation Across the Lysosome Membrane: Physiology, Pathology and Pharmacology
Lysosomes were discovered, almost 40 years ago, because their limiting membrane is impermeable to certain low molecular weight solutes. 2-Phosphoglycerol is such a substance and, as it is also a substrate for lysosomal acid phosphatase, lysosomes incubated with 2-phosphoglycerol hydrolyse it only if their membrane is ruptured. De Duve has described in a number of publications how this initial observation led on to the characterization of lysosomes as a distinct class of subcellular organelles (see, for example, De Duve 1969 and 1979).
KeywordsNeutral Amino Acid Cystine Level Cystine Residue Osmotic Protection Lysosomal Acid Phosphatase
Unable to display preview. Download preview PDF.
- Anonymous (1986) Lysosomal storage diseases. Lancet ii:898–899Google Scholar
- Bird SJ, Forster S, Lloyd JB (1988) Mechanism of dipeptide translocation across the lysosome membrane. Biochem Soc Trans 16:42–43Google Scholar
- Cabantchik ZI, Silfen J, Firestone RA, Krugliak M, Nissani E, Ginsburg H (1989) Effects of lyso-somotropic detergents on the human malarial parasite Plasmodium falciparium in in vitro culture. Biochem Pharmacol (in press)Google Scholar
- De Duve C (1969) The lysosome in retrospect. In: Dingle JT, Fell HB (eds) Lysosomes in biology and pathology, vol 1. Elsevier North Holland Biomédical Press, Amsterdam, pp 3–40Google Scholar
- Forster S, Lloyd JB (1988) Solute translocation across the mammalian lysosome membrane. Biochim Biophys Acta (in press)Google Scholar
- Förster S, Scarlett L, Lloyd JB (1988) Effects of a lysosomotropic detergent on cystinotic fibroblasts. Biochem Soc Trans 16:43–44Google Scholar
- Goldman R (1976) Ion distribution and membrane permeability in lysosomal suspensions. In: Dingle JT, Dean RT (eds) Lysosomes in biology and pathology, vol 5. Elsevier North Holland Biomédical Press, Amsterdam, pp 309–336Google Scholar
- Stein WD (1967) The movement of molecules across cell membranes. Academic Press, New York LondonGoogle Scholar