The Ontogeny of Hepatic Bile Acid Transport

  • F. J. Suchy
  • M. Ananthanarayanan
  • J. C. Bucuvalas
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Bile formation is an extraordinarily complex process that requires the participation of numerous transport mechanisms for organic and inorganic anions on the sinusoidal and canalicular domains of the hepatocyte plasma membrane. The vectorial transport of bile acids from sinusoidal blood into bile requires the polarized insertion of specific transport proteins into the basolateral and apical domains of the plasma membrane. Recent studies indicate that the carrier mechanisms for bile acids are ontogenically regulated and expressed at very specific times during perinatal development. These findings are consistent with observations in the intact animal showing that bile secretory function is immature at birth and develops during postnatal life (Suchy et al., 1987). In addition, hepatic uptake and elimination of bile acids and other organic anions are decreased in developing animals including the human compared with the adult.

Keywords

Hydrolysis Adenosine Polypeptide Polyacrylamide Alanine 

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References

  1. Bellerman P (1981) Amino acid transport and rubidium-ion uptake in monolayer cultures of hepatocytes from neonatal rats. Biochem J 198: 475–483Google Scholar
  2. Belknap WM, Zimmer-Nechemias L, Suchy FJ (1988) Bile acid efflux from suckling rat hepatocytes. Pediat Res 23: 364–367PubMedCrossRefGoogle Scholar
  3. Blitzer BL, Boyer JL (1982) Cellular mechanisms of bile format ion. Gastroenterology 82: 346–357PubMedGoogle Scholar
  4. Blitzer BL, Donovan CB (1984) A new method for the rapid isolation of basolateral plasma membrane vesicles from rat liver: characterization, validation, and bile acid transport studies. J Biol Chem 259: 9295–9301PubMedGoogle Scholar
  5. Fricker G, Hugentobler G, Meier PJ, Kurz G, Boyer JL (1987) Identification of a single sinusoidal bile salt uptake system in skate liver. Am J Physiol 253: G816–G822PubMedGoogle Scholar
  6. Inoue M, Kinne R, Tran T, Biempica L, Arias IM (1983) Rat liver canalicular membrane vesicles. Isolation and topographical characterization. J Biol Chem 258: 5183–5188PubMedGoogle Scholar
  7. Kramer W, Bickel U, Buscher HP, Gerok W, Kurz G (1982) Bile salt polypeptides in plasma membranes of hepatocytes revealed by photoaffinity labeling. Eur J Biochem 129: 12–24CrossRefGoogle Scholar
  8. Novak DA, Suchy FJ (1987) Postnatal expression of the canalicular bile acid transport system in rat liver. Hepatology 7: 1037 (Abstract)Google Scholar
  9. Piccoli DA, Muller ER, Vanderslice RR (1986) Maturation of biliary steady state uptake and secretion capacity in the perfused rat liver. Hepatology 5: 1185 (Abstract)Google Scholar
  10. Ricour C, Rey J (1972) Study of the hydrolysis and micellar solubilization of fats during intestinal perfusion. I. Results in the normal child. Europ J Clin Biol Res 17: 172–178Google Scholar
  11. Shaffer EA, Zahavi I, Gall DG (1985) Postnatal development of hepatic bile formation in the rabbit. Dig Dis Sci 30: 558–562PubMedCrossRefGoogle Scholar
  12. Stolz A, Sugiyama Y, Kuhlenkamp J (1986) Cytosolic bile acid binding protein in rat liver: radioimmunoassay, molecular forms, developmental characteristics and organ distribution. Hepatology 6: 433–439PubMedCrossRefGoogle Scholar
  13. Suchy FJ, Ananthanarayanan M, Bucuvalas JC, Osadchey B, Yamada T, Belknap W, Balistreri W, Kaplowitz N (1988) An antibody to a developmentally regulated 48 kDa liver plasma protein inhibits taurocholate by isolated rat hepatocytes. Gastroenterology 94: A596 (Abstract)Google Scholar
  14. Suchy FJ, Balistreri WF (1982) Uptake of taurocholate by hepatocytes isolated from developing rats. Pediat Res 16: 282–285PubMedCrossRefGoogle Scholar
  15. Suchy FJ, Bucuvalas JC, Goodrich A, Moyer MS, Blitzer BL (1986) Taurocholate transport and Na+, K+-ATPase activity in fetal and neonatal rat liver plasma membrane vesicles. Am J Physiol 251: G665–G673PubMedGoogle Scholar
  16. Suchy FJ, Bucuvalas JC, Novak DA (1987) Determinants of bile formation during development: ontogeny of hepatic bile acid metabolism and transport. Seminars Liver Dis 7: 77–84CrossRefGoogle Scholar
  17. Suchy FJ, Couchene SM, Blitzer BL (1985) Taurocholate trans-port by basolateral plasma membrane vesicles isolated from developing rat liver. Am J Physiol. 248: G648–G654PubMedGoogle Scholar
  18. Tavolini N, Jones NJT, Berk PD (1985) Postnatal development of bile secretory physiology in the dog. J Pediat Gastroent Nutr 4: 256–267CrossRefGoogle Scholar
  19. Van Dyke RW, Scharschmidt BF (1983) Na+,K+-ATPase mediated cation pumping in cultured rat hepatocytes: rapid modulation by alanine and taurocholate transport and characterization of its relationship to intracellular sodium concentration. J Biol Chem 258: 12912–12919PubMedGoogle Scholar
  20. Von Dippe P, Ananthanarayanan M, Levy D (1986) Purification and reconsti tut ion of the bile acid transport system from hepatocyte sinusoidal plasma membranes. Biochim Biophys Acta 862: 352–360CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • F. J. Suchy
    • 1
  • M. Ananthanarayanan
    • 1
  • J. C. Bucuvalas
    • 1
  1. 1.Division of Pediatric GastroenterologyChildren’s Hospital Medical CenterCincinnatiUSA

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