Microbiosensors for Clinical and Process Analysis

  • Isao Karube
  • Eiichi Tamiya
Conference paper

Abstract

Microbiosensors for urea and adenosine triphosphate (ATP) are prepared with ion sensitive field effect transistor (ISFET) modified by enzymes such as urease and H +-ATPase respectively. The Micro-urea sensor gives a linear relationship between the initial rate of the output gate voltage change and the logarithmic value of urea concentration between 16.7 and 167 mM. Determination of urea is possible within 30 s. The optimum pH is 6.0–6.5, at 37 °C. The system can be used for 20 d with only slight loss of enzymatic activity. The micro-ATP sensor can be used for the ATP determination. A linear relationship was obtained between the initial rate of change of the differential gate output voltage and the logarithm of the ATP concentration over the range 0.2–1.0 mM ATP-sensing system exhibited a response to 1 mM ATP for at least 18 d.

Keywords

Hydrolysis Peroxide Fermentation Urea Creatinine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    H. L. Resenthal, Anal. Chem. 27, 1980 (1955)CrossRefGoogle Scholar
  2. 2.
    R. L. Lemar and D. Bootzin, Anal. Chem. 29, 1233 (1957)CrossRefGoogle Scholar
  3. 3.
    R. A. Lienado and G. A. Rechnitz, Anal. Chem. 4, 1109 (1974)CrossRefGoogle Scholar
  4. 4.
    T. Matsuo and M. Esashi, Sens. Actuators 1, 11 (1981)Google Scholar
  5. 5.
    T. Bucher, Biochim. Biophys. Acta 292 (1947)Google Scholar
  6. 6.
    M. Deluca, in (A. Meister, Ed.), Advances in Enzymology, Wiley, New York 44, 37 (1976)Google Scholar
  7. 7.
    L. J. Blum, P. R. Coulet and D. C. Gautheron, Biotech. Bioeng. 27, 232 (1985)CrossRefGoogle Scholar
  8. 8.
    M. Yoshida, N. Sone, H. Hirata and Y. Kagawa, J. Biol. Chem. 250, 7910 (1975)Google Scholar
  9. 9.
    M. Yoshida, N. Sone, H. Hirata and Y. Kagawa, J. Biol. Chem. 252, 3480 (1977)Google Scholar
  10. 10.
    T. Hamamoto, K. Ohno and Y. Kagawa, J. Biochem. 91, 1759 (1982)Google Scholar
  11. 11.
    Y. Kagawa, J. Biochem. 95, 295 (1984)Google Scholar
  12. 12.
    Y. Miyahara, F. Matsu, T. Moriizumi, H. Matsuoka, I. Karube and S. Suzuki, in T. Seiyama, K. Fueki, J. Shiokawa and S. Suzuki (Eds.), Chemical Sensory, Kodansha, Tokyo, Elsevier, Amsterdam, p. 513, 1983Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Isao Karube
    • 1
  • Eiichi Tamiya
    • 1
  1. 1.Research Center for Advanced Science and TechnologyUniversity of TokyoMeguro-ku, TokyoJapan

Personalised recommendations