Abstract
The development of in vitro methods in biology has provided important tools for carrying out mechanistic studies. These techniques offer the advantage of studying biological responses under rigorously controlled experimental conditions and are particularly useful for the study of readily accessible cells (e.g., pulmonary macrophages). In addition, as more effective methodologies evolve, the numbers of experimental animals used for toxicological studies may be reduced. However, in vitro experimental systems do not reflect realistic exposure conditions and cannot simulate cell-cell interactions and corresponding microenvironments which are integral to a number of physiologic and pathologic events.
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© 1989 Springer-Verlag Berlin Heidelberg
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Warheit, D.B., Brody, A.R., Hartsky, M.A. (1989). Predictive Value of In Vitro Pulmonary Macrophage Functional Assays to Assess In Vivo Clearance of Inhaled Particles. In: Mossman, B.T., Bégin, R.O. (eds) Effects of Mineral Dusts on Cells. NATO ASI Series, vol 30. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74203-3_44
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DOI: https://doi.org/10.1007/978-3-642-74203-3_44
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