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Visualization by nlsLacZ of Gene Activity During Mouse Embryogenesis

  • Chapter
Vectors as Tools for the Study of Normal and Abnormal Growth and Differentiation

Part of the book series: NATO ASI Series ((ASIH,volume 34))

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Abstract

In mammals the embryo itself is derived from a few founder cells (reviewed in Mintz, 1974) that are specified at an as yet undefined stage before gastrulation. From these progenitors, many distinct cell types develop during the next few days. Their probable number is far greater than the number of described histological cell types. When the embryo progenitor cells are determined the genome has been epigenetically modified. Indeed, the male and female pronuclei display different properties (reviewed in Solter, 1987) due to distinct imprinting that occurs during gametogenesis (McGrath and Solter, 1984; Surani et al., 1984; reviewed in Monk, 1988). Further modifications of the zygotic genome probably occur during the early stages of embryogenesis, probably initiated by interactions between the three partners present at fertilization: the female cytoplasm and the two pronuclei. The relative importance of epigenetic modifications of regions of the chromosomes (by epigenetic, we mean any stably inherited modification of the genetic material such as methylation or special kind of secondary or tertiary configuration of the chromatin) compared to the repertoire of trans modulators in the control of the set of genes expressed in a particular cell is not easy to evaluate. Current hypotheses on the basis of these restrictions of potency involve cell position, cell interaction, cell lineage and timing (reviewed in Pedersen, 1986). Therefore, analysis of gene expression in relation to these parameters is of great interest.

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Authors and Affiliations

  1. Unité de Biologie Moléculaire du Développement, Institut Pasteur 28, rue du Docteur Roux, 75724, Paris Cédex 15, France

    J. F. Nicolas, C. Bonnerot, C. Kress, H. Jouin, P. Briand, G. Grimber & M. Vernet

  2. Laboratoire de Biochimie Génétique, Hôpital Necker, 149 rue de Sèvres, 75743, Paris Cédex 15, France

    P. Briand, G. Grimber & M. Vernet

Authors
  1. J. F. Nicolas
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  2. C. Bonnerot
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  3. C. Kress
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  4. H. Jouin
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  5. P. Briand
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  6. G. Grimber
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  7. M. Vernet
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Editors and Affiliations

  1. Heinrich-Pette-Institut für Virologie und Immunologie, Universität Hamburg, Martinistraße 52, 2000, Hamburg 20, Germany

    Heinz Lother , Rudolf Dernick  & Wolfram Ostertag ,  & 

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© 1989 Springer-Verlag Berlin Heidelberg

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Nicolas, J.F. et al. (1989). Visualization by nlsLacZ of Gene Activity During Mouse Embryogenesis. In: Lother, H., Dernick, R., Ostertag, W. (eds) Vectors as Tools for the Study of Normal and Abnormal Growth and Differentiation. NATO ASI Series, vol 34. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74197-5_4

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  • DOI: https://doi.org/10.1007/978-3-642-74197-5_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74199-9

  • Online ISBN: 978-3-642-74197-5

  • eBook Packages: Springer Book Archive

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