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Vectors as Tools for the Study of Normal and Abnormal Growth and Differentiation pp 209–219Cite as

Genetic Manipulation of Human Hematopoietic Stem Cells

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Part of the book series: NATO ASI Series ((ASIH,volume 34))

Abstract

Knowledge of the organization and regulation of the human stem cell hierarchy has lagged behind similar understanding in the mouse largely because of the lack of a suitable assay for human stem cells. Several new assays, based on transplantation of human hematopoietic cells into immune-deficient mice, have recently been developed which may detect human stem cells. Combining these assays with the high efficiency gene transfer technologies that have been developed using retrovirus vectors should prove to be a powerful approach to identifying the genetic events that govern the developmental program of human hematopoietic stem cells.

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Author information

Authors and Affiliations

  1. Department of Genetics, Research Institute, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada

    John E. Dick

  2. Department of Medical Genetics, University of Toronto, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada

    John E. Dick

  3. Department of Medical Biophysics, University of Toronto, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada

    John E. Dick

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  1. John E. Dick
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Editors and Affiliations

  1. Heinrich-Pette-Institut für Virologie und Immunologie, Universität Hamburg, Martinistraße 52, 2000, Hamburg 20, Germany

    Heinz Lother , Rudolf Dernick  & Wolfram Ostertag ,  & 

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© 1989 Springer-Verlag Berlin Heidelberg

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Dick, J.E. (1989). Genetic Manipulation of Human Hematopoietic Stem Cells. In: Lother, H., Dernick, R., Ostertag, W. (eds) Vectors as Tools for the Study of Normal and Abnormal Growth and Differentiation. NATO ASI Series, vol 34. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74197-5_20

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  • DOI: https://doi.org/10.1007/978-3-642-74197-5_20

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74199-9

  • Online ISBN: 978-3-642-74197-5

  • eBook Packages: Springer Book Archive

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