Lipid Transport in Plasmodium falciparum -Infected Erythrocyte Membranes

  • Kasturi Haldar
Conference paper
Part of the NATO ASI Series book series (volume 40)


During its intraerythrocytic development the malaria parasite induces changes in the transport of phospholipids across the host erythrocyte membrane. In the mature erythrocyte, protein mediated transport of phosphatidylethanolamine (PE) and phosphatidylserine (PS) to the cytoplasmic leaflet is an energy requiring process and appears to serve as the primary mechanism for maintaining phospholipid asymmetry at the plasma membrane (Daleke and Huestis, 1985; Tilley et. al., 1986; Zachowski et. al., 1986) Band 7 has been identified as the putative amino-phospholipid translocator in human erythrocytes (Connor and Schroit 1988). Human erythrocyte membranes do not contain a phosphatidylcholine (PC) transporter and PC transbilayer movement in these membranes is extremely slow (Op den Kamp, 1979). In erythrocytes infected with the malaria parasitesPlasmodium falciparum and Plasmodium knowlesi, there is an increased uptake and exchangeability of exogenously added radiolabeled PS, PE and PC with lipids of the host cell membrane (van der Schaft et. al., 1987; Moll et. al., 1988). However little is known about the molecular basis of these changes in parasitised erythrocytes. We have investigated the movement of fluorescent analogues of phospholipids in parasitised erythrocytes to elucidate mechanisms of lipid transport from the red cell membrane to the intracellular parasite.


Erythrocyte Membrane Uninfected Cell Infected Erythrocyte Flip Flop Host Cell Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Connor J and Schroit AJ (1988) Transbilayer movement of phosphatidylserine in erythrocytes: inhibition of transport and preferential labelling of a 31,000-dalton protein by sulfhydril reactive reagents. Biochemistry 27:848–851PubMedCrossRefGoogle Scholar
  2. Daleke DL and Huestis WH (1985) Incorporation and translocation of amino-phospholipids in human erythrocytes. Biochemistry 24:5406–5416PubMedCrossRefGoogle Scholar
  3. Gupta CM and Mishra GC (1981) Transbilayerphospholipid asymmetry in Plasmodium knowlesi-infected host cell membrane Science (Wash. DC) 212:1047–1049Google Scholar
  4. Gupta CM, Alam A, Mathur, PN and Dutta GP (1982) A new look at non parasitised red cells of malaria infected monkeys. Nature (Lond) 299:259–261CrossRefGoogle Scholar
  5. Haldar K, de Amorim AF and Cross GAM (1989) Transport of fluorescent phospholipid analogues from the erythrocyte membrane to the parasite in Plasmodium falciparum-infected cells. J. Cell Biol. 108: 2183–2192PubMedCrossRefGoogle Scholar
  6. Joshi P, Alam A, Chandra R Puri SK and Gupta CM (1986) Possible basis for membrane changes in non parasitised erythrocytes of malaria infected animals. Biochim. Biophys. Acta. 862:220–222.CrossRefGoogle Scholar
  7. Kates M (1975) Techniques of lipidology. In Laboratory Techniques in Biochemistry and Molecular Biology, editors Work TS and Work E vol 3: 558–565Google Scholar
  8. Martin OC, and Pagano RE (1987) Transbilayer movement of fluorescent analogues of phosphatidylserine and phosphatidylethanolamine at the plasma membrane of cultured cells. J. Biol. Chem. 262: 5890–5898.PubMedGoogle Scholar
  9. Moll G N, Vial HJ, Ancelin ML, Op den Kamp JAF, Roelofson B and van Deenen LL (1988) Phospholipid uptake by Plasmodium knowlesi infected erythrocytes. FEB S Lett. 232:341–346CrossRefGoogle Scholar
  10. Op den Kamp JAF (1979) Lipid asymmetry in membranes. Ann. Rev. Biochem. 48: 47–71CrossRefGoogle Scholar
  11. Schwartz RS, Olson JA, Ravento-Suarez C, Yee M, Heath RH, Lubin B and Nagel RL (1987) Altered plasma membrane phospholipid organisation in Plasmodium falciparum-infected erythrocytes. Blood 69:401–407PubMedGoogle Scholar
  12. Simoes AP, Moll GN, Vial HJ, Roelofsen B and Op den Kamp JAF (1989) On the phosphatidylcholine molecular species composition in Plasmodium knowlesi infected erythrocyte membranes. Submitted for publicationGoogle Scholar
  13. Sleight RG and Pagano, RE (1984) Transport of fluorescent phosphatidylcholine analogue from the plasma membrane to the golgi apparatus. J. Cell Biol. 99:742–751PubMedCrossRefGoogle Scholar
  14. Sleight RG and Pagano RE (1985) Transbilayer movement of a fluorescent phosphatidylethanolamine analogue across the plasma membrane of cultutred mammalian cells. J. Biol. Chem. 26:1146–1154Google Scholar
  15. Tilley L, Cribier S, Roelofsen B, Op den Kamp JAF and van Deenen LL (1986) ATP dependent translocation of aminophospholipids across the human erythrocyte membrane. FEBS Lett. 194:21–27PubMedCrossRefGoogle Scholar
  16. van der Schaft PH, Beaumelle B, Vial BH, Roelofsen B, Op den Kamp JJ and van Deenen LL (1987) Phospholipid organisation in monkey erythrocytes uponPlasmodium knowlesi infection. Biochim. Biophys. Acta. 901:1–14PubMedCrossRefGoogle Scholar
  17. Vial HJ, Thuet MJ andPhillipot JR (1982) Phospholipid biosynthesis in synchronous Plasmodium falciparum cultures. J. Parasitol. 68: 379–391PubMedCrossRefGoogle Scholar
  18. Zachowski A, Favre E, Cribier S, Herve P and Devaux P (1986) Outside-inside translocation of aminophospholipids in the human erythrocyte membrane is mediated by a specific enzyme. Biochemistry 25: 2585–2590PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Kasturi Haldar
    • 1
  1. 1.Dept. Microbiology and ImmunologyStanford UniversityStanfordUSA

Personalised recommendations