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Evidence for an Intermembrane Transport of Lipids to Peripheral Nerve Myelin in vivo by a Novel Pulsed Wave Procedure

  • Anthony Heape
  • Françoise Boiron
  • Claude Cassagne
Conference paper
Part of the NATO ASI Series book series (volume 40)

Abstract

Three principal methods have been employed for the study of intracellular transport in nervous tissues: autoradiography associated with electron microscopy, time-staggered double-isotope labelling and isotopic pulse-chase experiments. Although the autoradiographical (Gould and Dawson, 1976; Gould et al., 1987; Kumara-Siri and Gould, 1980; Rawlins, 1973) and the double-labelling methods (Konat et al., 1985; Pereyra et al., 1983; Shimomura et al., 1984) can provide useful complementary information, only the isotopic pulse-chase experiments (Benjamins and Iwata, 1979; Burkart et al., 1982; Konat, 1981; Konat et al., 1985), associated with the use of intracellular transport inhibitors, would be able to demonstrate unambiguously the existence of intermembrane transfer events implicated in membrane biogenesis. No in vivo studies of peripheral nerves have been carried out using this method, most probably because of the difficulty in controlling the experimental conditions.

Keywords

Sciatic Nerve Polar Lipid Membrane Fraction Sucrose Gradient Intraneural Injection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Anthony Heape
    • 1
  • Françoise Boiron
    • 1
  • Claude Cassagne
    • 1
  1. 1.Institut de Biologie Cellulaire et Neurochimie du CNRSBordeaux cedexFrance

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