Evidence for an Intermembrane Transport of Lipids to Peripheral Nerve Myelin in vivo by a Novel Pulsed Wave Procedure

  • Anthony Heape
  • Françoise Boiron
  • Claude Cassagne
Conference paper
Part of the NATO ASI Series book series (volume 40)


Three principal methods have been employed for the study of intracellular transport in nervous tissues: autoradiography associated with electron microscopy, time-staggered double-isotope labelling and isotopic pulse-chase experiments. Although the autoradiographical (Gould and Dawson, 1976; Gould et al., 1987; Kumara-Siri and Gould, 1980; Rawlins, 1973) and the double-labelling methods (Konat et al., 1985; Pereyra et al., 1983; Shimomura et al., 1984) can provide useful complementary information, only the isotopic pulse-chase experiments (Benjamins and Iwata, 1979; Burkart et al., 1982; Konat, 1981; Konat et al., 1985), associated with the use of intracellular transport inhibitors, would be able to demonstrate unambiguously the existence of intermembrane transfer events implicated in membrane biogenesis. No in vivo studies of peripheral nerves have been carried out using this method, most probably because of the difficulty in controlling the experimental conditions.


Sciatic Nerve Polar Lipid Membrane Fraction Sucrose Gradient Intraneural Injection 
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  1. Benjamins JA, Iwata R (1979) Kinetics of entry of galactolipids and phospholipids into myelin. J Neurochem 32:921–926PubMedCrossRefGoogle Scholar
  2. Boiron F, Darriet D, Bourre JM, Cassagne C (1984) Decreased biosynthesis of saturated C20-C24 fatty acids by the Trembler mouse sciatic nerve. Neurochem Int 6:109–116PubMedCrossRefGoogle Scholar
  3. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254PubMedCrossRefGoogle Scholar
  4. Burkart T, Caimi L, Siegrist HP, Herschkowitz NN, Wiesmann UN (1982) Vesicular transport of sulfatide in the myelinating mouse brain. Functional association with lysosomes. J Biol Chem 257:3151–3156PubMedGoogle Scholar
  5. Gould RM, Dawson RMC (1976) Incorporation of newly formed lecithin into peripheral nerve myelin. J Cell Biol 68:480–496PubMedCrossRefGoogle Scholar
  6. Gould RM, Holshek J, Silverman W, Spivak WD (1987) Localization of phospholipid synthesis to Schwann cells and axons. J Neurochem 48:1121–1131PubMedCrossRefGoogle Scholar
  7. Heape MA, Juguelin H, Boiron F, Cassagne C (1985) An improved one-dimensional thin-layer chromatographic technique for polar lipids. J Chromat 322:391–395CrossRefGoogle Scholar
  8. Heape A, Boiron F, Cassagne C (1989) High uptake and rapid metabolism of palmitate in peripheral nerves of normal and Trembler mice in vivo: similarities and differences. Neurochem Int: In press Google Scholar
  9. Konat G (1981) Intracellular translocation of newly synthesized myelin proteins in the rat brain stem slices. Exp Neurol 73:254–266PubMedCrossRefGoogle Scholar
  10. Konat G, Divkaran P, Samorajski T, Wiggins RC (1985) Intracellular translocation of myelin proteolipid protein. J Neurochem 44: 1500–1510PubMedCrossRefGoogle Scholar
  11. Kumara-Siri MH, Gould RM (1980) Enzymes of phospholipid synthesis: axonal versus Schwann cell distribution. Brain Res 186:315–330PubMedCrossRefGoogle Scholar
  12. Pereyra PM, Braun PE, Greenfield S, Hogan (1983) Studies on subcellular fractions which are involved in myelin assembly: labeling of myelin proteins by a double radio-isotope approach indicates developmental relationships. J Neurochem 41:974–988PubMedCrossRefGoogle Scholar
  13. Pleasure DE, Prockop DJ (1972) Myelin synthesis in peripheral nerve in vitro: sulphatide incorporation requires a transport lipoprotein. J Neurochem 19:283–295PubMedCrossRefGoogle Scholar
  14. Radominska-Pyrek A, Strosznajder J, Dabrowiecki Z Goracci G, Chojnacki T, Horrocks LA (1977) Enzymic synthesis of ether types of choline and ethanolamine phosphoglycerides by microsomal fractions from rat and liver. J Lipid Res 18:53–58PubMedGoogle Scholar
  15. Rawlins FA (1973) A time-sequence autoradiographic study of the in vivo incorporation of [1,2-3H] cholesterol into peripheral nerve myelin. J Cell Biol 58:42–53PubMedCrossRefGoogle Scholar
  16. Shimomura K, Yahara S, Kishimoto Y, Benjamins J A (1984) Metabolism of cerebroside and sulfatides in subcellular fractions of developing rat brain. Biochim Biophys Acta 795:265–270PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Anthony Heape
    • 1
  • Françoise Boiron
    • 1
  • Claude Cassagne
    • 1
  1. 1.Institut de Biologie Cellulaire et Neurochimie du CNRSBordeaux cedexFrance

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