Mutants: A Fruitful Approach to the Understanding of Asparagine-Linked Glycosylation in Eukaryotic Cells
Proteins which have glycans attached on one to six asparagine residues (N-linked glycoproteins) are important constituents of eukaryotic cells. They occur as soluble or membrane-associated components either inside or outside of cells. Secreted proteins such as thyroglobulin and immunoglobulins and intravesicular proteins such as the lysosomal glycosidases are N-linked glycoproteins. Many membrane-associated proteins found on the cell-surface are this class of glycoproteins — insulin receptor, EGF receptor, LDL receptor, and transferrin receptor, as are enzymes associated with many cellular membranes such as Na+/K+ ATPase, galactosyl transferase, sialyl transferase and HMG CoA reductase. Fibronectin and laminin, proteins found at the cell surface, also contain N-linked glycans.
KeywordsChinese Hamster Ovary Cell Asparagine Residue Cholesteryl Oleate Glycosylation Reaction Dolichyl Phosphate
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