Replication of Plant RNA Viruses in Vivo in Relation to Virus-Host Interactions
Various approaches can be used to examine the replication of plant RNA viruses within the host. The modifications incurred by the different cell compartments due to virus infection can indicate the parts of the cell likely to be involved in replication. The sites at which RNA replication occurs can also be ascertained by investigating where virus-specific, newly synthesized or double-stranded RNA species are located, or where the viral nonstructural proteins can be detected. Characterization and analysis of purified double-stranded RNAs has helped to elucidate the mechanism for the synthesis of the coat protein subgenomic RNA. Enzyme complexes (RNA-dependent RNA polymerase or replicase) responsible for RNA replication have been isolated. Although difficult to work with, they provide valuable information regarding the virus-coded nonstructural proteins that participate in RNA replication. The characterization of host-encoded factor(s) possibly involved in this process remains a major problem.
KeywordsHydrolysis Phenol Codon Hexagonal Polypeptide
Unable to display preview. Download preview PDF.
- Francki RIB (1987) Responses of plant cells to virus infection with special reference to the sites of RNA replication. In: Brinton MA and Rueckert RR (eds) Positive strand RNA viruses. Alan R liss Inc., New York, p 423Google Scholar
- Gargouri R, Joshi RL, Bol JF, Astier-Manifacier S, Haenni AL (1989) Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo. Virology, in pressGoogle Scholar
- Jordan RL, Dodds JA (1985) Double-stranded RNA in detection of diseases of known and unproven viral etiology. Acta Hortic 164:101–108Google Scholar
- Lafleche D, Bové JM (1986) Sites d’incorporation de l’uridine tritiée dans les cellules du parenchyme foliaire de Brassica chinensis ,saines ou infectées par le virus de la mosaïque jaune du navet. C R Acad Sci 266:1839–1841Google Scholar
- Laflèche D, Bové C, Dupont G, Mouchès C, Astier T, Garnier M, Bové JM (1972) Site of viral RNA replication in the cells of higher plants: TYMV-RNA synthesis on the chloroplast outer membrane system. Proc Fed Eur Biochem Soc 27:43–71Google Scholar
- Martelli GP, Russo M (1984) Use of thin sectioning for visualization and identification of plant viruses. Methods Virol 8:143–224Google Scholar