Abstract
The accumulation of the pterocarpan derivate glyceollin I is an early response of soybean (Glycine max) roots to infection with an incompatible race of the fungus Phvtophthora megasperma f.sp. glvcinea (Pmg) and is correlated with the inhibition of fungal growth (Hahn et al 1985). Starting from 4-coumaroyl-CoA and malonyl-CoA the biosynthesis of glyceollin involves nine enzymatic steps, which have all been characterized. For purification of enzymes soybean cell suspension cultures challenged with a Pmg or yeast elicitor were used in most cases.
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References
Arcy-Lameta A (1986) Study of soybean and lentil root exudates. II. Identification of some polyphenols compounds, relation with plantlet physiology. Plant and Soil 92: 113–123
Ayabe S, Udajgawa A, Furuya T (1988a) NAD(P)H-Dependent 6-deoxychalcone synthase activity in Glvcvrrhiza echinata cells induced by yeast extract. Arch Biochem Biophys 261: 458–462
Ayabe S, Udagawa A, Furuya T (1988b) Stimulation of chalcone synthase activity by yeast extract in cultured Glvcvrrhiza echinata cells and 5-deoxyflavanone formation by isolated protoplasts. Plant Cell Reports 7:35–38
Bleß W, Barz W (1988) Isolation of pterocarpan synthase, the terminal enzyme of pterocarpan phytoalexin biosynthesis in cell suspension cultures of Cicer arietinum. FEBS Lett 235: 47–50
Douce R, Block M A, Dorne A J, Joyard J (1984) The plastid envelope membranes; their structure, composition, and role in chloroplast biogenesis. In: Roodyn D B (ed) Subcellular biochemistry. Vol 10, Plenum Press New York, p 1–84
Ebel J, Grisebach H, Bonhoff A, Grab D, Hoffmann Ch, Kochs G, Mieth, H, Schmidt W, Stäb M (1986) Phytoalexin synthesis in soybean following infection of roots with Phytophthora megasperma or treatment of cell cultures with fungal elicitor. In: Lugtenberg B (ed) Recognition in microbe-plant symbiotic and pathogenic interactions. NATO ASI series, Vol H4, Springer-Verlag Berlin Heidelberg, p 345–361
Grisebach H, Brandner G (1961) Zur Biogenese der Isoflavone. IV. Mitt.: Über die Biogenese von Biochanin A und Formononetin in der Kichererbse (Cicer arietinum L.) Z Naturforsch 16b: 2–5
Hagmann M L, Heller W, Grisebach H (1984) Induction of phytoalexin synthesis in soybean: Stereospecific 3,9-dihydroxypterocarpan 6a-hydroxylase from elicitor-induced soybean cell cultures. Eur J Biochem 142: 127–931
Hahn M G, Bonhoff A, Grisebach H (1985) Quantitative localization of the phytoalexin glyceollin I in relation to fungal hyphae in soybean roots infected with Phytophthora megasperma f.sp. glvcinea. Plant Physiol 77: 591–601
Hakamatsuka T, Noguchi H, Ebizuka Y, Sankawa U (1988) Deoxychalcone synthase from cell suspension cultures of Pueraria lobata. Chem Pharm Bull 36: 4225–4228
Hinderer W, Flentje U, Barz W (1987) Microsomal isoflavone 2’- and 3’-hydroxylases from chickpea (Cicer arietinum L.) cell suspension induced for pterocarpan phytoalexin formation. FEBS Lett 214:101–106
Kochs G, Grisebach H (1986) Enzymic synthesis of isoflavones. Eur J Biochem 155: 311–318
Kochs G, Grisebach H (1989) Phytoalexin synthesis in soybean. Purification and reconstitution of cytochrome P-450 3,9-dihydroxypterocarpan 6a-hydroxylase and separation from cytochrome P-450 cinnamate 4-hydroxylase. Arch Biochem Biophys, in press
Kosslak R M, Bookland R, Barkei J, Paaren, H E, Appelbaum E R (1987) Induction of Bradyrhizobium japonicum common nod genes by isoflavones isolated from Glycine max. Proc Natl Acad Sci USA 84: 7428–7432
Tiemann K, Hinderer W, Barz W (1987) Isolation of NADPH: isoflavone oxidoreductase, a new enzyme of pterocarpan phytoalexin biosynthesis in cell suspension cultures of Cicer arietinum. FEBS Lett 213: 324–328
Welle R, Grisebach H (1988a) Isolation of a novel NADPH-dependent reductase which coacts with chalcone synthase in the biosynthesis of 6’-deoxychalcone. FEBS Lett 236: 221–225
Welle R, Grisebach H (1988b) Induction of phytoalexin synthesis in soybean: enzymatic cyclization of prenylated pterocarpans to glyceollin isomers. Arch Biochem Biophys 263:191–198
Welle R, Grisebach H (1989) Phytoalexin synthesis in soybean cells: Elicitor induction of reductase involved in biosynthesis of 6’-deoxychalcone. Arch Biochem Biophys 272: 97–102
Werner D, Mellor R B, Hahn M G, Grisebach H (1985) Soybean root response to symbiotic infection. Glyceollin I accumulation in an ineffective type of soybean nodules with an early loss or the peribacteroid membrane. Z. Naturforsch 40c: 179–181
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© 1989 Springer-Verlag Berlin Heidelberg
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Grisebach, H., Edelmann, L., Fischer, D., Kochs, G., Welle, R. (1989). Biosynthesis of Phytoalexins and Nod-Gene Inducing Isoflavones in Soybean. In: Lugtenberg, B.J.J. (eds) Signal Molecules in Plants and Plant-Microbe Interactions. NATO ASI Series, vol 36. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74158-6_5
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DOI: https://doi.org/10.1007/978-3-642-74158-6_5
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