Phosphorylation of the Nicotinic Acetylcholine Receptor and Localization of Its Phosphorylation Sites

  • Anat Safran
  • Carlo Provenzano
  • Ronit Sagi-Eisenberg
  • Sara Fuchs
Conference paper
Part of the NATO ASI Series book series (volume 32)


The nicotinic acetylcholine receptor (AChR) mediates synaptic transmission at the post-synaptic membrane of nicotinic cholinergic synapses. This receptor is a prototype of the chemically-gated ion channel receptors which contain the ligand binding site as well as an intrinsic ion channel responsible for signal transduction (Hucho, 1986; Changeux et al., 1987). Phosphorylation of AChR has been implicated in playing a role in the regulation of its ion channeling. Cyclic AMP-elevating agents which activate the cAMP-dependent protein kinase (PKA), as well as phorbol esters, which activate the Ca2+/phospholipid-dependent protein kinase (PKC), were shown to enhance receptor desensitizaticn (Middleton et al., 1986; Albuquerque et al., 1986; Eusebi et al., 1985). It was postulated that direct phosphorylation of the AChR by these two kinases may result in the observed physiological response. Indeed, phosphorylation of the receptor was demonstrated in intact mammalian muscle cells and this phosphorylation was stimulated by cAMP and Ca2+ (Miles et al., 1987; Smith et al., 1987).


Nicotinic Acetylcholine Receptor Torpedo Electric Organ Mammalian Muscle Cell Consensus Recognition Site Nicotinic Cholinergic Synapse 
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  1. Albuquerque EX, Deshpande SS, Aracava Y, Alkondon M, Daly JW (1986) FEBS Letter 199: 113–120.CrossRefGoogle Scholar
  2. Campbell DG, Hardie DG, Vulliet PR (1986) J Biol Chan 261: 10489–10492.Google Scholar
  3. Changeux JP, Giraudat J, Dennis M (1987) Trends in Pharm Sci 8: 459–465.CrossRefGoogle Scholar
  4. Eusebi F, Molinaro M, Zani BM (1985) J Cell Biol 100: 1339–1342.PubMedCrossRefGoogle Scholar
  5. Fuchs S, Neumann D, Safran A (1986) In: Nato ASI Series, Vol H3 Nicotinic Acetylcholine Receptor, A. Maelicke (ed) Springer-Verlag, Berlin, Heidelberg, pp 49–59.Google Scholar
  6. Gordon AS, Davis OG, Milfay D, Diamond I (1977) Nature 267: 539–540.PubMedCrossRefGoogle Scholar
  7. Hucho F (1986) Eur J Biochem 158: 211–226.PubMedCrossRefGoogle Scholar
  8. Huganir RL, Delcour AH, Greengard P, Hess GP (1986) Nature 321: 774–776.PubMedCrossRefGoogle Scholar
  9. Huganir RL, Greengard P (1983) Proc. Natl. Acad. Sci. USA 80: 1130–1134.PubMedCrossRefGoogle Scholar
  10. Huganir RL, Miles K, Greengard P (1984) Proc. Natl. Acad. Sci. USA 81: 6968–6972.PubMedCrossRefGoogle Scholar
  11. Kemp BE, Graves, DJ, Benjamini E, Krebs EG (1977) J Biol Chem 252: 4888–4894.PubMedGoogle Scholar
  12. Kishimoto A, Nishiyama K, Nakanishi H, Uratsuji Y, Nomura H, Takeyama Y, Nishizuka Y (1985) J Biol Chem 260: 12492–12499.PubMedGoogle Scholar
  13. Middleton P, Jaramillo F, Schuetze SM (1986) Proc. Natl. Acad. Sci. USA 83: 4967–4971.PubMedCrossRefGoogle Scholar
  14. Miles K, Anthony DT, Rubin LL, Greengard P, Huganir, RL (1987) Proc. Natl. Acad. Sci. USA 84: 6591–6595.PubMedCrossRefGoogle Scholar
  15. Nishizuka, Y (1986) Science 233: 305–312.PubMedCrossRefGoogle Scholar
  16. Safran A, Neumann D, Fuchs S (1986) EMBO J 5: 3175–3178.PubMedGoogle Scholar
  17. Safran A, Sagi-Eisehberg R, Neumann D, Fuchs S (1987) J Biol Chem 262: 10506–10510.PubMedGoogle Scholar
  18. Sibley DR, Benovic JL, Caron MG, Lefkowitz RJ (1988) Endocrine Rev 9: 38–56.CrossRefGoogle Scholar
  19. Smith MVI, Merlie JP, Lawrence JC (1987) Proc. Natl. Acad. Sci. USA 84: 6601–6605.PubMedCrossRefGoogle Scholar
  20. Souroujon, MC, Neumann D, Pizzighella S, Fridkin M, Fuchs S (1986) EMBO J 5: 543–546.PubMedGoogle Scholar
  21. Teichberg VI, Sobel A, Changeux JP (1977) Nature 267: 540–542.PubMedCrossRefGoogle Scholar
  22. Yee GH, Huganir RL (1987) J Biol Chem 262: 16748–16753.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Anat Safran
    • 1
  • Carlo Provenzano
    • 1
  • Ronit Sagi-Eisenberg
    • 1
  • Sara Fuchs
    • 1
  1. 1.Department of Chemical ImmunologyThe Weizmann Institute of ScienceRehovotIsrael

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