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Phosphorylation of the Nicotinic Acetylcholine Receptor and Localization of Its Phosphorylation Sites

  • Anat Safran
  • Carlo Provenzano
  • Ronit Sagi-Eisenberg
  • Sara Fuchs
Conference paper
Part of the NATO ASI Series book series (volume 32)

Abstract

The nicotinic acetylcholine receptor (AChR) mediates synaptic transmission at the post-synaptic membrane of nicotinic cholinergic synapses. This receptor is a prototype of the chemically-gated ion channel receptors which contain the ligand binding site as well as an intrinsic ion channel responsible for signal transduction (Hucho, 1986; Changeux et al., 1987). Phosphorylation of AChR has been implicated in playing a role in the regulation of its ion channeling. Cyclic AMP-elevating agents which activate the cAMP-dependent protein kinase (PKA), as well as phorbol esters, which activate the Ca2+/phospholipid-dependent protein kinase (PKC), were shown to enhance receptor desensitizaticn (Middleton et al., 1986; Albuquerque et al., 1986; Eusebi et al., 1985). It was postulated that direct phosphorylation of the AChR by these two kinases may result in the observed physiological response. Indeed, phosphorylation of the receptor was demonstrated in intact mammalian muscle cells and this phosphorylation was stimulated by cAMP and Ca2+ (Miles et al., 1987; Smith et al., 1987).

Keywords

Nicotinic Acetylcholine Receptor Torpedo Electric Organ Mammalian Muscle Cell Consensus Recognition Site Nicotinic Cholinergic Synapse 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Anat Safran
    • 1
  • Carlo Provenzano
    • 1
  • Ronit Sagi-Eisenberg
    • 1
  • Sara Fuchs
    • 1
  1. 1.Department of Chemical ImmunologyThe Weizmann Institute of ScienceRehovotIsrael

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