Structure-Function Correlation: Amacrine Cells of Fish and Amphibian Retinae

  • Mustafa B. A. Djamgoz
  • Silvana Vallerga
Part of the NATO ASI Series book series (volume 31)


Morphological diversity of amacrine cells (ACs) is clearly a general characteristic of vertebrate retinae. Beginning with the comparative work of Cajal (1893), much detailed information concerning the cells’ morphological features in both the plane as well as the radial extent of the retina has been obtained by Golgi impregnation (Boycott and Dowling 1969; Naka and Carraway, 1975, Perry and Walker, 1980; Ammermuller and Weiler, 1981; Famiglietti and Vaughn, 1981; Kolb et al., 1981; Vallerga and Deplano, 1984; Wagner and Wagner, 1988). The larger the number of parameters considered, the more numerous the types of AC that became morphologically differentiated. Thus, in the retina of the turtle, Kolb (1982) distinguished 22 types of AC, and in the retina of the cyprinid fish, roach, 43 types of AC were found (Wagner and Wagner, 1988). In contrast, an attempt to rationalize the morphological diversity of ACs using dendritic architecture as a qualitative parameter, as opposed to quantitative parameters, such as extent of dendritic field, size, etc. resulted in the definition of five classes of ACs in the sparid fish, bogue (Vallerga and Deplano, 1984). As regards their light-evoked responses, ACs have been divided most commonly into two broad categories: sustained and transient, in turn, the former could be depolarizing or hyperpolarizing (Kaneko and Hashimoto, 1969; Kaneko, 1973, Toyoda et al., 1973; Chan and Naka, 1975, Famiglietti et al., 1977, Murakami and Shimoda, 1977; Mitarai et al., 1978, Djamgoz et al., 1988a,).


Amacrine Cell Plexiform Layer Inner Plexiform Layer Cyprinid Fish Tiger Salamander 
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  1. Ammermuller J, Weiler R (1981) The ramification pattern of amacrine cells within the inner plexiform layer of the carp retina. Cell Tissue Res 220: 699–723PubMedCrossRefGoogle Scholar
  2. Barlow HB and Levick WR (1965) The mechanisms of directionally selective units in rabbit’s retina: J Physiol 178: 477–504Google Scholar
  3. Boycott BB, Dowling JE (1969) Organization of the primate retina: Light microscopy, Phil Trans R Soc Lond 255: 109–184Google Scholar
  4. Cajal, S. R. (1893) La retine des vertebres. La Cellule 9: 121–225.Google Scholar
  5. Chan RY, Naka K-I (1976) The amacrine cell. Vision Res 16: 1119–1129PubMedCrossRefGoogle Scholar
  6. Dacey DM (1988) Dopamine-accumulating retinal neurones revealed by in vitro fluorescence display a unique morphology. Science 240: 1196–1198PubMedCrossRefGoogle Scholar
  7. Deplano S, Vallerga S (1984) Quantitative morphology of amacrine cells in teleost retina. In: Borsellino A, Cervetto L (eds) Photoreceptors, Plenum Publ Co, p 295–317Google Scholar
  8. Djamgoz MBA (1984) Electrophysiological characterization of the spectral sensitivities of the horizontal cells in cyprinid fish retina. Vision Res 24: 1677–1687PubMedCrossRefGoogle Scholar
  9. Djamgoz MBA, Ruddock KH (1978) Properties of amacrine cell responses recorded from isolated fish retinae. J Physiol Lond 7: 89–93Google Scholar
  10. Djamgoz MBA, Ruddock KH (1983) Spectral characteristic of transient amacrine cells in a cyprinid fish (roach) retina in vitro. J. Physiol., Lond. 339: 19P.Google Scholar
  11. Djamgoz MBA, Downing JEG, Wagner E, Wagner H-J, Zeutzius I (1985) Functional organization of amacrine cells in the teleost fish retina. In: Gallego A, Gouras P (eds) Neurocircuitry of the Retina: A Cajal Memorial, Elsevier New York.Google Scholar
  12. Djamgoz MBA, Downing JEG, Wagner H-J (1987) Retinal neurones of cyprinid fish: Intracellular marking with horseradish peroxidase and correlative morphological analysis. Exp. Biol. 46: 203–216.Google Scholar
  13. Djamgoz MBA, Downing JEG, Wagner H-J (1988a) An intracellular horseradish peroxidase study of amacrine cells in the retina of a cyprinid fish, (submitted)Google Scholar
  14. Djamgoz MBA, Spadavecchia L, Usai C, Vallerga S (1988b) Light- evoked response types and morphological identification of amacrine cells in goldfish retina, (submitted)Google Scholar
  15. Dowling JE, Werblin FS (1969) Organization of the retina of the mudpuppy, Necturus maculosus. II Intracellular recordings. J Neurophysiol 32: 339–355Google Scholar
  16. Famiglietti EV, Kaneko A, Tachibana M (1977). Neuronal architecture of ‘on’ and off1 pathways to ganglion cells in carp retina. Science 198: 1267–1269.PubMedCrossRefGoogle Scholar
  17. Famiglietti EV, Kolb H (1976) Structural basis of ‘ON’and ‘OFF’-centre responses in retinal ganglion cells. Science 194: 193–195CrossRefGoogle Scholar
  18. Famiglietti EV, Vaughn JE (1981) Golgi-impregnated amacrine cells and GABAergic retinal neurons: immunocytochemical and histochemical stratification in the inner plexiform layer of of rat retina. J Comp Neurol 197: 129–139PubMedCrossRefGoogle Scholar
  19. Kaneko A (1970) Physiological and morphological identification of horizontal, bipolars and amacrine cells in goldfish retina. J Physiol Lond 207: 623–633.PubMedGoogle Scholar
  20. Kaneko A (1973) Receptive field organization of bipolar and amacrine cells in the goldfish retina. J Physiol Lond 235: 133–153.PubMedGoogle Scholar
  21. Kaneko A, Hashimoto H (1969) Electrophysiological study of single neurones in the inner nuclear layer of the carp retina. Vision Res 9: 37–55.PubMedCrossRefGoogle Scholar
  22. Kolb H (1982) The morphology of bipolar cells, amacrine cells and ganglion cells in the retina of the turtle, Pseudemys scripta elegans. Phil Trans R Soc Lond B 298: 355–393CrossRefGoogle Scholar
  23. Kolb H, Nelson R (1981) Amacrine cells of the catfish retina. Vision Res 21: 1625–1633CrossRefGoogle Scholar
  24. Marc RE, Liu WS, Muller JF (1988) Gap junctions in the inner plexiform layer of the goldfish retina. Vision Res 28: 9–24Google Scholar
  25. Marchiafava PL and Torre V (1980) The responses of amacrine cells to light and intracellularly applied currents. J Physiol Lond 251: 83–102Google Scholar
  26. Mitarai G, Goto T, Takagi S (1978) Receptive field arrangement of colour-opponent bipolar and amacrine cells in carp retina. Sensory Processes 2: 375–382PubMedGoogle Scholar
  27. Murakami M, Shimoda Y (1977) Identification of amacrine and ganglion cells in the carp retina. J Physiol Lond 265: 801–818.Google Scholar
  28. Naka K-I (1980) A class of catfish amacrine cells respond preferentially to objects which move vertically. Vision Res 20: 961–965PubMedCrossRefGoogle Scholar
  29. Naka K-I, Carraway NRG (1975) Morphological and functional identification of catfish retinal neurons: I Classical morphology. J Neurophysiol 38: 53–71Google Scholar
  30. Naka K-I, Christensen BN (1981) Direct electrical connections between transient amacrine cells in the catfish retina. Science 214: 462–464PubMedCrossRefGoogle Scholar
  31. Naka K-I, Ohtsuka T (1975) Morphological and functional identifications of catfish retinal neurons. II Morphological identification. J Neurophysiol 38: 72–91Google Scholar
  32. Perry VH, Walker M (1980) Amacrine cells, displaced amacrine cells and interplexiform cells in the retina of the rat. Proc R Soc Lond B 208: 413–431Google Scholar
  33. Teranishi T, Negishi K, Kato S (1987) Functional and morphological correlates of amacrine cells in carp retina. Neurosci 20: 935–950CrossRefGoogle Scholar
  34. Toyoda J, Hashimoto H, Ohtsu K (1973) Bipolar-amacrine transmission in the carp retina. Vision Res 13: 295–307.PubMedCrossRefGoogle Scholar
  35. Vallerga S (1981) Physiological and morphological identification of amacrine cells in the retina of the larval tiger salamander. Vision Res 21: 1307–1317PubMedCrossRefGoogle Scholar
  36. Vallerga S, Deplano S (1984) Differentiation, extent and layering of amacrine cell dendrites in the retina of a sparid fish. Proc R Soc Lond B 221: 465–477PubMedCrossRefGoogle Scholar
  37. Vaney D (1986) Morphological identification of serotonin accumulating neurons in the living retina. Science 233: 444–446PubMedCrossRefGoogle Scholar
  38. Yang CY, Yazulla S (1996) Neuropeptide-like immunoreactive cells in the retina of the larval tiger salamander: attention to the symmetry of dendritic projections. J Comp Neurol 248: 105–118CrossRefGoogle Scholar
  39. Yagi T, Kaneko A (1988) The axon terminal of goldfish retinal horizontal cells: a low membrane conductance measured in solitary preparations and its implications to signal conductance from the soma. J Neurophysiol 59: 482–494PubMedGoogle Scholar
  40. Wagner H-J, Wagner E (1988) Amacrine cells in the retina of a teleost fish. The roach (Rutilus rutilus). Phil Trans R Soc Lond B (in press)Google Scholar
  41. Werblin FS (1970) Responses of retinal cells to moving spots intracellular recordings in Necturus maculosus. J Neurophysiol 33: 342–350PubMedGoogle Scholar
  42. Werblin FS (1972) Lateral interactions at the inner plexiform layer of vertebrate retina. Antagonistic response to change. Science 175: 1008–1010PubMedCrossRefGoogle Scholar
  43. Werblin FS (1978) Transmission along and between rods in the retina of the tiger salamander. J Physiol Lond 294: 613–626.Google Scholar
  44. Werblin FS, McGuire G, Lukasiewicz P, Eliasof S, Wu SM (1988) Neural interaction mediating the detection on motion in the retina of the tiger salamander. J Visual Neurosci 1Google Scholar
  45. Wong-Riley MTT (1974) Synaptic organization of the inner plexiform layer inn the retina of the larval tiger salamander. J Neurocytol 3: 1–33PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Mustafa B. A. Djamgoz
    • 1
  • Silvana Vallerga
    • 2
  1. 1.Department of Pure and Applied Biology, Neurobiology GroupImperial CollegeLondonUK
  2. 2.Istituto di Cibernetica e Biofisica del Consiglio Nazionale delle RicercheGenovaItaly

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