Quantitative Jamin-Lebedeff interference microscopy can be used to measure the distribution of lignin in sections of wood or pulp fibers. The technique is fully quantitative, accurate, and can be calibrated with any desired solid state lignin standard. Resolution is adequate to measure lignin concentration in the cell corner middle lamella (ccml) and S2 regions of the specimen (Boutelje 1972, Donaldson 1985a), while a modification of the basic technique allows measurements to be made in other cell wall regions (Donaldson 1987). Interference microscopy is ideally suited to the study of lignification during cell development, lignin removal during pulping, and any other application where information on lignin concentration at the cellular level is required.
KeywordsRefractive Index Secondary Wall Middle Lamella Effective Thickness Lignin Concentration
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