Many staining techniques have been used in combination with light microscopy to study the distribution of lignin in wood (Brauns 1952). However, since the advent of electron microscopy, considerable efforts have been made to obtain a better understanding of lignin morphology in wood cell walls. One of the techniques in electron microscopy for detection of lignin involves the use of potassium permanganate (KMnO4) stains (Crocker 1921, Hepler et al. 1970, Bland et al. 1971, Parham 1974, Kutscha and Schwarzmann 1975, Saka et al. 1979). Although some doubts have been expressed as to its specificity for lignin (Hoffmann and Parameswaran 1976, Kishi et al. 1982), the KMnO4 staining technique provides many excellent details of the ultrastructural features of lignin in wood.


Secondary Wall Compression Wood Middle Lamella Lignin Concentration Wood Cell Wall 
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© Springer-Verlag Berlin Heidelberg 1992

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  • S. Saka

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