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Molecular Characterization of a Transforming Retrovirus Involved in Pre-B Cell Lymphomas

  • W. Y. Langdon
  • J. W. Hartley
  • S. P. Klinken
  • S. K. Ruscetti
  • H. C. MorseIII
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 141)

Abstract

Cas-Br-M is a biologically cloned ecotropic murine leukemia virus [MuLV] originally isolated from wild mice of the Lake Casitas region of California. Inoculation of Cas-Br-M into newborn NFS/N mice induces hematopoietic neoplasms. The tumors are evident at 18–30 weeks and include T- and B-cell lymphomas, myeloid leukemias and erythroleukemias [Fredrickson et al, 1984]. it is unclear how Cas-Br-M is able to generate such a wide spectrum of hematopoietic neoplasms, although envelope-recombinant mink cell focus-forming [MCF] MuLVs are involved in some of these tumors [Holmes et al, 1986]. This was determined by preparing cell-free extracts from a range of tumors and injecting the extracts into newborn NFS/N mice. It was reasoned that if Cas-Br-M was the etiological agent then the mice should develop a range of tumor phenotypes with long latencies. If, however, MCF viruses were involved, the mice should develop tumors with the same phenotype as the extracted tumor and with reduced latency. Of 12 extracts examined, three appeared to support the latter hypothesis [Holmes et al, 1986]. On further examination one of these extracts, called Cas NS-1, was found to contain a virus capable of transforming mouse fibroblasts.

Keywords

Wild Mouse Transforming Virus Cell Surface Antigen Expression Ecotropic Murine Leukemia Virus Transform Mouse Fibroblast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Fredrickson TN, Langdon WY, Hoffman PM, Hartley JW and Morse H C III (1984) Histologic and cell surface antigen expression of hematopoietic tumors induced by Cas-Br-M murine leukemia virus. JNCI 72:447–454PubMedGoogle Scholar
  2. Holmes KL, Langdon WY, Fredrickson TN, Coffman RL, Hoffman PM, Hartley JW and Morse HC III (1986) Analysis of neoplasms induced by Cas-Br-M tumor extracts. J Immunol 137:679–688PubMedGoogle Scholar
  3. Langdon WY, Hartley JW, Holmes KH, Fredrickson TN and Morse HC III (1984) Identification of a transforming virus from a lymphoma of a mouse infected with a wild mouse retrovirus. Curr Topics Microbiol Immunol 113:241–249CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1988

Authors and Affiliations

  • W. Y. Langdon
    • 1
  • J. W. Hartley
    • 2
  • S. P. Klinken
    • 1
  • S. K. Ruscetti
    • 3
  • H. C. MorseIII
    • 2
  1. 1.Walter and Eliza Hall Institute of Medical ResearchPO Royal Melbourne HospitalAustralia
  2. 2.Laboratory of ImmunopathologyNIHBethesdaUSA
  3. 3.Laboratory of GeneticsNIHBethesdaUSA

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