Current State of the Structural Analysis of the Actin: DNase I Complex

Kabsch, W.; Pai, E. F.; Mannherz, H. G.; Suck, D.

doi:10.1007/978-3-642-73925-5_7

W. Kabsch⁴,
E. F. Pai⁴,
H. G. Mannherz⁵ &
…
D. Suck⁶

Part of the book series: Springer Series in Biophysics ((BIOPHYSICS,volume 3))

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Abstract

The complex between rabbit skeletal muscle actin and bovine pancreatic DNase I (Lazarides & Lindberg, 1974) can be crystallized in three different forms. The x-ray structure of the orthorhombic form III has been solved to a resolution of 0.6nm (Suck, Kabsch & Mannherz, 1981). More recently the resolution has been increased to 0.45nm and the actin molecule has been unambigously identified in the map (Kabsch, Mannherz & Suck, 1985). This identification was derived from knowledge of the DNase I atomic structure (Suck, Oefner & Kabsch, 1984). In addition, only one combination of actin and DNase I density was found which is common to both the monoclinic (form II) and the orthorhombic (form III) crystals. It was concluded that this unique combination must represent the complex as found in solution.

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References

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Authors and Affiliations

Abt. Biophysik, Max-Planck-Institut für medizinische Forschung, Jahnstr. 29, D-6900, Heidelberg 1, Germany
W. Kabsch & E. F. Pai
Institut für Anatomie und Zellbiologie der Universität, D-3550, Marburg, Germany
H. G. Mannherz
EMBL, Postfach 102209, D-6900, Heidelberg 1, Germany
D. Suck

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W. Kabsch
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E. F. Pai
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H. G. Mannherz
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D. Suck
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Editors and Affiliations

Maurice E. Müller-Institut, Biozentrum der Universität Basel, Klingelbergstraße 70, CH-4056, Basel, Germany
Ueli Aebi
Abteilung für Biophysikalische Chemie, Biozentrum der Universität Basel, Klingelbergstraße 70, CH-4056, Basel, Germany
Jürgen Engel

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Kabsch, W., Pai, E.F., Mannherz, H.G., Suck, D. (1989). Current State of the Structural Analysis of the Actin: DNase I Complex. In: Aebi, U., Engel, J. (eds) Cytoskeletal and Extracellular Proteins. Springer Series in Biophysics, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73925-5_7

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DOI: https://doi.org/10.1007/978-3-642-73925-5_7
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