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A Modular Model of the Nuclear Pore Complex

  • Christopher W. Akey
Part of the Springer Series in Biophysics book series (BIOPHYSICS, volume 3)

Abstract

The nuclear pore complex (NPC) is a singular assembly which spans the double membrane of the nuclear envelope and mediates nucleocytoplasmic transport (for a review see Newport & Forbes, 1987). In order to understand these transport processes, I am currently devising methods to solve the 3-dimensional structures of various NPCs, including forms with a dilated central channel (transporter). The first step in this process is determining the 2-dimensional structures and possible interrelationships of the different classes of NPCs observed in isolated amphibian nuclear envelopes. Cryo-electron microscopy of frozen-hydrated specimens, coupled with image analysis of a 4700 particle globally aligned database, has allowed verification of the NPC model proposed by Unwin & Milligan (1982). The model has been extended to 50-60 angstrom resolution and new features relevant to the in vivo function of the assembly have been observed. Significantly, projected structures of the NPC-transporter in different transport-related states have been obtained and an initial model is proposed.

Keywords

Nuclear Envelope Nuclear Pore Complex Central Pore Nucleocytoplasmic Transport Modular Model 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Akey, C.W. (submitted) Interactions, structure and conformational flexibility of the Nuclear Pore Complex revealed by cryo-electron microscopy.Google Scholar
  2. Akey, C.W. & D.S. Goldfarb (submitted). Nuclear import requires docking and subsequent dilation of the Nuclear Pore Complex-Transporter.Google Scholar
  3. Feldherr, C.M., Kallenbach,E. & Schultz, N. (1984). Movement of a karyophilic protein through the Nuclear Pores of oocytes. J. Cell Biol. 99: 2216–2222.Google Scholar
  4. Milligan, R.A. (1986). A structural model of the Nuclear Pore Complex. In Nucleocytoplasmic Transport (ed. R. Peters & M. Trendelenburg ), Springer-Verlag, pp. 113–122.CrossRefGoogle Scholar
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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Christopher W. Akey
    • 1
  1. 1.MRC Laboratory of Molecular BiologyCambridgeEngland

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