Complex Formation of Endothelial Cell Thrombospondin with Heparin and Fibronectin

  • R. Dardik
  • J. Lahav
Conference paper
Part of the Springer Series in Biophysics book series (BIOPHYSICS, volume 3)


Thrombospondin (TSP) is a large glycoprotein synthesized, secreted and incorporated into the extracellular matrix by various cell types in culture (see Silvenstein et al.,1986 for a recent review). TSP synthesis is inversely proportional to cell density (Mumby et al.,1984a). Its matrix incorporation is age-dependent (Kramer et al.,1985) and can be regulated by specific growth factors (Majack et al.,1985), suggesting that TSP is involved in cell growth and development. A recent report shows that cell-associated TSP is essential for smooth muscle cell proliferation (Majack et al., 1988). TSP was found to promote cell-substrate adhesion of several human sqamous carcinoma cell lines, but not that of melanoma or glioma cell lines (Varani et al.,1986). Among the tumor cell lines tested there was direct correlation between the ability of the cells to synthesize and bind TSP and their ability to utilize the protein as an adhesion factor (Riser et al.,1988). It seems, however, that such correlation is not a general phenomenon, since endothelial cells, capable of both TSP synthesis and binding, do not adhere to TSP covered surfaces. Moreover, TSP was shown to inhibit cell-substrate adhesion of endothelial cells (Lahav et al., 1987; Lahav,1988). TSP is a multifunctional protein capable of interacting with a variety of macromolecules, among them fibronectin (FN) (Lahav et al., 1982; Lahav et al.,1984), heparin (Lawler et al.,1981), collagen (Lahav et al.,1982; Mumby et al.,1984b) and sulfated glycolipids (Roberts et al.,1985).


Heparin Binding Heparin Binding Site Cell BioI Human Squamous Carcinoma Cell Sulfated Glycolipid 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • R. Dardik
    • 1
  • J. Lahav
    • 1
  1. 1.Department of Polymer ResearchThe Weizmann Institute of ScienceRehovotIsrael

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