Abstract
Microtubules are fibrous elements, made of a dimeric protein (tubulin), found ubiquitously in eucaryotic cells where they perform a wide variety of physiological functions. They are centrally involved in mitosis and other motility related phenomena such as the transport of subcellular organelles or changes in cell shape (Snyder & Mc Intosh, 1976 ; Dustin, 1978 ; Margolis & Wilson, 1981).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Asnes C.F. and Wilson L. (1979). Isolation of bovine brain amicrotubule protein without glycerol: polymerisation kinetics change during purification cycles. Anal. Biochem., 98: 64–73.
Dustin P. (1978). Microtubules ( Springer Verlag, New-York).
Job D. and Margolis R.L. (1984). Isolation from bovine brain of a superstable microtubule subpopulation with microtubule seeding activity. Biochemistry, 23: 3025–3031.
Job D., Pabion M. and Margolis R.L. (1985). Generation of microtubule stability subclasses by microtubule-associated proteins: implications for the microtubule “dynamic instability” model. J. Cell. Biol., 101: 1680–1689.
Laemmli I.K. and Favre M. (1973). Maturation of the head of bacteriophage T4: I. DNA packaging events. J. Mol. Biol., 80: 575–580.
Margolis R.L. and Wilson L. (1981). Microtubule treadmills - possible molecular machinery. Nature (London) 292: 705–711.
Snyder 3.A. and McIntosh J.R. (1976). Biochemistry and physiology of microtubules. Ann. Rev. Biochem., 45: 699–720.
Wilson L., Snyder K.B., Thompson W.C. and Margolis R.L. (1982). A rapid filtration assay for analysis of microtubule assembly, disassembly, and steady-state tubulin flux. Methods Cell. Biol., 24: 159–169.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Pirollet, F., Job, D. (1988). Assay of in Vitro Microtubule Assembly. Effect of Microtubule Associated Proteins. In: Latruffe, N., Gaudemer, Y., Vignais, P., Azzi, A. (eds) Dynamics of Membrane Proteins and Cellular Energetics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73905-7_13
Download citation
DOI: https://doi.org/10.1007/978-3-642-73905-7_13
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-50047-6
Online ISBN: 978-3-642-73905-7
eBook Packages: Springer Book Archive