Rhodopsin and G-Protein Linked Receptors
Integral membrane proteins present formidable challenges not only to crystallization techniques but also to the well-established procedures of sequence analysis particularly the area of peptide purification. This short review illustrates the way in which protein chemistry can be employed to arrive at a 3-dimensional representation of an integral membrane protein. The object was to provide a test-bed with which to design and interpret protein engineering studies. Such investigations will in turn help to refine or correct the model thereby generating a structure which may be a usefully close approximation of the real situation.
KeywordsCysteine Fractionation Lysine Polypeptide Tryptophan
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