Immuno-chemical Recognition of Synthetic Peptides Based on the Sequence and Three-dimensional Structure of Human Renin: An Immuno-control of Renin Activity
Renin (EC 184.108.40.206), an aspartyl protease, is a rate limiting factor on angiotensin II production which is a potent vasoconstrictor peptide implicated in the regulation of blood pressure and electrolyte metabolism. So renin is an important target in the design of antihypertensive drugs. Different approaches in the inhibition of the renin-angiotensinogen system have been adopted, such as the use of substrate analogues (Szelke et al., 1982), statine-containing peptides (Boger et al., 1983, 1985; Guegan et al., 1986), peptides derived from the renin profragment sequence (Cumin et al., 1985) and specific monoclonal renin antibodies (Galen et al., 1984). Recently, specific blockade of the renin-angiotensinogen system has been obtained by active immunization against renin in normotensive marmoset (Michel et al., 1987) but this active immunization induces an autoimmune desease, so it was worthwhile to conduct experiments with synthetic peptides related to renin sequence and to try to determinate the major renin epitopes.
KeywordsSynthetic Peptide Renin Activity Disulfide Bridge Cyclic Peptide Renin Inhibitor
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