The Long Search for a Viable Method of C-terminal Sequencing
The history of C-terminal protein sequencing dates back at least to 1926 when the thiocyanate degradation was introduced by Schlack and Kumpf (see reviews by Loudon et al 1977; Ward 1986; Rangarajan 1988 for citations of original literature). Many chemical methods have been introduced since then, touted by their inventors and by the few who later improved them. But these methods have rarely contributed essential - or even useful - sequence information and none have demonstrated the capacity for extended degradations with all common residues. But recent improvements in a few of the half-dozen or so currently active methods have convinced many that the grail of C-terminal sequencing is at last within reach. When I began to prepare for this Conference, I had hoped to be able to compare the performance of these methods with small test substrates, but unfortunately, time and resources did not permit.
KeywordsEdman Degradation Protein Sequence Analysis Cleavage Efficiency Methyl Sulfate Yield Amino Acid
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