The Long Search for a Viable Method of C-terminal Sequencing
The history of C-terminal protein sequencing dates back at least to 1926 when the thiocyanate degradation was introduced by Schlack and Kumpf (see reviews by Loudon et al 1977; Ward 1986; Rangarajan 1988 for citations of original literature). Many chemical methods have been introduced since then, touted by their inventors and by the few who later improved them. But these methods have rarely contributed essential - or even useful - sequence information and none have demonstrated the capacity for extended degradations with all common residues. But recent improvements in a few of the half-dozen or so currently active methods have convinced many that the grail of C-terminal sequencing is at last within reach. When I began to prepare for this Conference, I had hoped to be able to compare the performance of these methods with small test substrates, but unfortunately, time and resources did not permit.
KeywordsCarboxyl Polypeptide Angiotensin Anhydride Hunt
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- Black SD, Coon MJ (1986) Comparative structures of P-450 cytochromes. In: Montellano PRO (ed) Cytochrome P-450 Plenum, p 161Google Scholar
- Hayashi R (1988) Enzymatic methods of protein/peptide sequencing from carboxyterminal end. In: Bhown AS (ed) Protein/peptide sequence analysis. CRC Press, Boca Raton FL, p 145Google Scholar
- Hunt DF, Yates JR, Shabanowitz J (1987) Protein sequencing by tandem mass spectrometry. In: Walsh KA (ed) Methods in protein sequence analysis 1986. Humana, Clifton NJ, p 149Google Scholar
- Johnson L, Tarr G (1987) C-terminal sequence of proteins: rapid isolation and Edman sequencing of C-terminal peptides from digests. In: Walsh KA (ed) Methods in protein sequence analysis 1986. Humana, Clifton NJ, p 351Google Scholar
- Klemm P (1984) Carboxy-terminal sequence determination of proteins and peptides with carboxypeptidase Y. In: Walker JM (ed) Methods in molecular biology vol 1. Humana, p 255Google Scholar
- Krutzsch HC (1988) Protein/peptide sequence analysis by mass spectrometry. In: Bhown AS (ed) Protein/peptide sequence analysis. CRC Press, Boca Raton FL, p 161Google Scholar
- Loudon GM, Parham ME, Miller MJ (1977) Approaches to carboxy-terminal sequencing and end-group determinations in peptides. In: van Tamelen EE (ed) Perspectives in bioorganic chemistry. Academic Press, New York, v 3Google Scholar
- Rangarajan M (1988) Chemical methods of amino acid sequence analysis from carboxyterminal end. In: Bhown AS (ed) Protein/peptide sequence analysis. CRC Press, Boca Raton FL, p 135Google Scholar
- Ringe D (1988) An extra dimension to NMR Nature 332: 303Google Scholar
- Ward CW (1986) Carboxy terminal sequence analysis. In: Darbre A (ed) Practical protein chemistry. John Wiley, London, p 491Google Scholar