Camellia sinensis L. (Tea): In Vitro Regeneration

Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 7)


Tea is the oldest caffeine-containing beverage, and has been used for two or three thousand years in south-east China (Eden 1958). It is also the most widely consumed hot beverage, as being the cheapest. To date, the habit of tea drinking has become well established for more than half of the world’s population. The most common type of tea is black or fermented tea. The unfermented type is green tea, which is produced in China and Japan, and gives a distinctive taste different from black tea. A further semi-fermented tea is oolong, consumed in China and Japan. In some few cases tea leaves are used as vegetables, like leppet tea in Burma and meing tea in Thailand. Based on FAO records (1984), 2.7 million hectares of land are under tea cultivation and 2.2 million tons are produced every year in the world. Over 80% of the world’s tea exports come from India and Sri Lanka. Exports from China rank next to these two countries. The United Kingdom is the largest importer of the world, importing over 20% of the production. The second largest importer is the United States, but there only about half a pound per person each year is drunk.


Somatic Embryo Stem Segment Epidermal Layer Camellia Sinensis Purine Alkaloid 
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  1. Bagratishvili DG, Zaprometov MN, Butenko RG (1979) Obtaining a cell suspension culture from the tea plant. Fiziol Rast 26: 449–451.Google Scholar
  2. Barone JJ, Roberts H (1984) Human consumption of caffeine. In Dews PB (ed) Caffeine. Springer, Berlin Heidelberg New York, pp 59–73.CrossRefGoogle Scholar
  3. Baumann TW, Frischknecht PM (1987) Purines: In: Constabel F, Vasil IK (ed) Cell culture and somatic cell genetics of plants, vol 5. Phytochemicals in cultured cell. Academic Press, London New York.Google Scholar
  4. Bennet WY, Scheibert P (1982) In vitro generation of callus and plantlets from cotyledons of Camellia japonica. Camellia J 37: 12–15.Google Scholar
  5. Bokuchava MA, Skobeleva NI (1980) The biochemistry and technology of tea manufacture. CRC Critical Reviews in Food Science and Nutrition, vol 11: 304–369.Google Scholar
  6. Carlisi JC, Torres KC (1986) In vitro shoot proliferation of Camellia purple Dawn. Hort Sci 21: 314.Google Scholar
  7. Chen Z, Liao H (1983) A success in bringing out tea plants from the anthers. China Tea 5: 6–7.Google Scholar
  8. Chlyah H (1974) Inter-tissue correlation in organ fragments. Organogenetic capacity of tissue excised from stem segments of Torenia fournieri Lind cultured separately in vitro. Plant Physiol 54: 341–348.PubMedCrossRefGoogle Scholar
  9. Crézé J (1980) Camellia cultivation in vitro. Int Camellia J 12: 31–34.Google Scholar
  10. Doi Y (1980) Suitable cultural conditions for callus induction from stem segments of tea and their application to anther culture. Stud Tea 58: 1–9.Google Scholar
  11. Doi Y (1981) Frequency of root differentiation in anther culture of tea. Stud Tea 60: 1–3.Google Scholar
  12. Eden T (1958) The development of tea culture. In: Eden T (ed) Tea. Longman, London, pp 1–4.Google Scholar
  13. FAO (ed) (1982) Trade yearbook, vol 36. FAO, Rome.Google Scholar
  14. FAO (ed) (1984) Production yearbook, vol 38. FAO, Rome.Google Scholar
  15. Hamaya E (1981) Diseases of tea plants in Japan and their control. Plant Protect Res 14: 96–111.Google Scholar
  16. Hamaya E (1982) Tricome infection of the tea anthracnose fungus Gloeosporium theae-sinensis. JARQ 16: 114–118.Google Scholar
  17. Kato M (1982) Results of organ culture on Camellia japonica and C. sinensis. Jpn J Breed 32 (Suppl 2): 267–277.Google Scholar
  18. Kato M (1985) Regeneration of plantlets from tea stem callus. Jpn J Breed 35: 317–322.Google Scholar
  19. Kato M (1986a) Micropropagation through cotyledon culture in Camellia japonica L. and C. sinensis L. Jpn J Breed 36: 31–38.Google Scholar
  20. Kato M (1986 b) Micropropagation through cotyledon culture in Camellia sasanqua. Jpn J Breed 36 (Suppl 1): 82–83.Google Scholar
  21. Kato M (1986 c) Regeneration of plantlets from pre-cultured tea stem callus. Jpn J Breed 36 (Suppl 2): 278–279.Google Scholar
  22. Kihlman BA (1977) Caffeine containing beverages. In: Kihlman BA (ed) Caffeine and chromosomes. Elsevier, Amsterdam, pp 33–51.Google Scholar
  23. Nadamitsu S, Andoh Y, Kondo K, Segawa M (1986) Interspecific hybrids between Camellia vietnamensis and C. chrysantha by cotyledon culture. Jpn J Breed 36: 309–313.Google Scholar
  24. Ogutuga DBA, Northcote DH (1970a) Caffeine formation in tea callus tissue. J Exp Bot 21: 258–273.CrossRefGoogle Scholar
  25. Ogutuga DBA, Northcote DH (1970b) Biosynthesis of caffeine in tea callus tissue. Biochem J 117: 715–720.PubMedGoogle Scholar
  26. Purseglove JW (1974) Theaceae. In: Purseglove JW (ed) Tropical crops. Dicotyledons. Longman, London, pp 599–612.Google Scholar
  27. Samartin A, Vieitez AM, Vieitez E (1984) In vitro propagation of Camellia japonica seedlings. Hort Sci 19: 225–226.Google Scholar
  28. Samartin A, Vieitez AM, Vieitez E (1986) Rooting of tissue cultured camellias. J Hortic Sci 61: 113–120.Google Scholar
  29. Shimokado T, Murata T, Miyaji Y (1986) Formation of embryoid by anther culture of tea. Jpn J Breed 36 (Suppl 2): 282–283.Google Scholar
  30. Suzuki T, Takahashi E (1976) Metabolism of methionine and biosynthesis of caffeine in the tea plant (Camellia sinensis L.) Biochem J 160:171–179.PubMedGoogle Scholar
  31. Suzuki T, Waller GR (1988) MetaboUsm and analysis of caffeine and other methylxanthines in coffee, tea, cola, guarana and cacao. In: Linskens HF (ed) Modern method of plant analysis, New Ser, vol 8. Springer, Berlin Heidelberg New York Tokyo.Google Scholar
  32. Tavartkiladze OK, Kutubidze VV (1984) In vitro culture of lateral buds of Thea sinensis. Abstr Int Symp Plant tissue and cell culture application to crop improvement.Olomouc, Czech, p 189.Google Scholar
  33. Tran Than Van M (1973) In vitro control of de novo flower, bud, root and callus differentiation from excised epidermal tissues. Nature (London) 246: 44–45.CrossRefGoogle Scholar
  34. Tsushida T, Doi Y (1984) Caffeine, theanine and catechin content in calluses of tea stem and anther. Nippon Nogeikagaku Kaishi 58: 1131–1133.CrossRefGoogle Scholar
  35. Wu C-T, Huang T-K, Chen G-R, Chen S-Y (1981) A review on the tissue culture of tea plants and on the utilization of callus-derived plants. In: Rao AN (ed) Tissue culture of economically important plants. Proc COSTED Symp, Singapore, pp 104-106.Google Scholar
  36. Zhuang C, Liang H (1985) Somatic embryogenesis and plantlets formation in cotyledon culture of Camellia chrysantha. Acta Bot Yunn 7: 446–457.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • M. Kato
    • 1
  1. 1.Faculty of AgricultureYamaguchi UniversityYamaguchi 753Japan

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