Ultrastructure of Human Breast Carcinoma from a Pleural Effusion

  • S. Scarpa
  • A. Modesti
  • G. D’Orazi
  • F. Massari
  • M. Matzeu
Conference paper

Abstract

The fluid aspirated from pleural effusions contains various cells including lymphocytes, polymorphonuclear leukocytes, macrophages, mesothelial cells, fibroblasts, and a few tumor cells in neoplastic patients (Hofmann et al. 1984). The cause of the effusion is often unknown and the tumoral component is often insufficient to make a certain cytologic diagnosis. We propose that the cellular component should be analyzed ultrastructurally to characterize the cell histotypes. Furthermore, the few tumor cells present in the fluid can be plated in vitro for a short-term cell culture, offering a valid source of fresh living cells to study better the specific causes of the initial pleural effusion.

Keywords

Citrate Hematoxylin Eosin Uranyl Laminin 

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References

  1. Birembaut P, Caron Y, Adnet JJ (1985) Usefulness of basement membrane markers in tumoural pathology. J Pathol 145: 283–296PubMedCrossRefGoogle Scholar
  2. Hofmann V, Berens ME, Früh U (1984) Analysis of malignant effusions by cellular composition, proliferation kinetics, and in vitro clonogenicity. Recent Results Cancer Res 94: 35–40PubMedGoogle Scholar
  3. Nesland JM, Holm R, Lunde S, Johannessen JV (1987) Diagnostic problems in breast pathology: the benefit of ultrastructural and immunocytochemical analysis. Ultrastruct Pathol 11: 293–311PubMedCrossRefGoogle Scholar
  4. Osborn M, Weber K (1983) Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology. Lab Invest 48: 372–394PubMedGoogle Scholar
  5. Rizk NN, El-Rakhawy MT (1981) Tissue culture and scanning electron microscopy of breast carcinoma cell line MCF-7 from pleural effusion. Acta Anat (Basel) 109: 70–74CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • S. Scarpa
  • A. Modesti
  • G. D’Orazi
  • F. Massari
  • M. Matzeu

There are no affiliations available

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